Louis G. Woodhams

Ciliary wavelength has its limits

Louis G. Woodhams

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Theoretical relationships between axoneme distortion and internal forces and torques in ciliary beating

Louis G. Woodhams, Philip V. Bayly

The axoneme is an intricate nanomachine responsible for generating the propulsive oscillations of cilia and flagella in an astonishing variety of organisms. New imaging techniques based on cryoelectron-tomography (cryo-ET) and subtomogram averaging have revealed the detailed structures of the axoneme and its …

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Virtual blebbistatin: A robust and rapid software approach to motion artifact removal in optical mapping of cardiomyocytes

Louis G. Woodhams, Jingxuan Guo, David Schuftan, John J. Boyle et autres

Fluorescent reporters of cardiac electrophysiology provide valuable information on heart cell and tissue function. However, motion artifacts caused by cardiac muscle contraction interfere with accurate measurement of fluorescence signals. Although drugs such as blebbistatin can be applied to stop cardiac tissue from …

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Abstract P1096: Computational Motion Artifact Removal Acts As Virtual Blebbistatin In Ipsc-derived Cardiomyocytes And Micro-tissues

Louis G. Woodhams, Jingxuan Guo, Nathaniel Huebsch, Guy M. Genin

Introduction: Drugs like blebbistatin that paralyze tissues are critical for accurate optocardiography but directly affect sarcomeres, making it challenging to study structure-function relationships. To overcome this, we applied Direct Deformation Estimation (DDE), a robust strain estimation tool, to enable computational motion removal …

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List of contributors

Lea M. Alford, Prachee Avasthi, Mayanka Awasthi, Philip V. Bayly et autres

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Physics and mechanics of ciliary beating

Louis G. Woodhams, Dario Cortese, Philip V. Bayly, Kirsty Y. Wan

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Basal bodies bend in response to ciliary forces

Anthony D. Junker, Louis G. Woodhams, Adam W. J. Soh, Eileen O’Toole et autres

Motile cilia beat with an asymmetric waveform consisting of a power stroke that generates a propulsive force and a recovery stroke that returns the cilium back to the start. Cilia are anchored to the cell cortex by basal bodies (BBs) that are …

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Fluorescence imaging of beta cell primary cilia

Zipeng A. Li, Jung Hoon Cho, Louis G. Woodhams, Jing W. Hughes

Primary cilia are slender cell-surface organelles that project into the intercellular space. In pancreatic beta cells, primary cilia coordinate a variety of cell responses including GPCR signaling, calcium influx, and insulin secretion, along with likely many underappreciated roles in islet development and …

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Islet primary cilia motility controls insulin secretion

Jung Hoon Cho, Zipeng A. Li, Lifei Zhu, Brian D. Muegge et autres

Primary cilia are specialized cell-surface organelles that mediate sensory perception and, in contrast to motile cilia and flagella, are thought to lack motility function. Here, we show that primary cilia in human and mouse pancreatic islets exhibit movement that is required for …

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Intracellular connections between basal bodies promote the coordinated behavior of motile cilia

Adam W. J. Soh, Louis G. Woodhams, Anthony D. Junker, Cassidy M. Enloe et autres

Whether intracellular connections between basal bodies control ciliary behavior in multiciliated cells is investigated. Using a Tetrahymena live cell immobilization technique to quantify ciliary dynamics, it is shown that inter-BB connections are required for effective ciliary waveform and coordinated ciliary beating that …

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Generation of ciliary beating by steady dynein activity: the effects of inter-filament coupling in multi-filament models

Louis G. Woodhams, Yenan Shen, Philip V. Bayly

The structure of the axoneme in motile cilia and flagella is emerging with increasing detail from high-resolution imaging, but the mechanism by which the axoneme creates oscillatory, propulsive motion remains mysterious. It has recently been proposed that this motion may be caused …

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Intracellular connections between basal bodies promote the coordinated behavior of motile cilia

Adam W. J. Soh, Louis G. Woodhams, Anthony D. Junker, Cassidy M. Enloe et autres

Summary Hydrodynamic flow produced by multi-ciliated cells is critical for fluid circulation and cell motility. Hundreds of cilia beat with metachronal synchrony for fluid flow. Cilia-driven fluid flow produces extracellular hydrodynamic forces that cause neighboring cilia to beat in a synchronized manner. …

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