Ryoji Nagai

Preferential formation of N ω -(carboxyethyl)arginine from methylglyoxal and its accumulation in human lens proteins

Himeno Takahashi, Mime Nagai, Tsuyoshi Ikeda, Ken Hoshiyama et autres

Methylglyoxal (MGO), a highly reactive dicarbonyl compound generated mainly by glycolysis, is a major precursor of advanced glycation end products (AGEs). Although lysine-derived AGEs, such as Nε-(carboxyethyl)lysine (CEL), have been widely used as markers of MGO-mediated protein modification, increasing evidence suggests that …

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Fumarate drives inflammatory crosstalk between adipocytes and macrophages to promote foam cell formation

Himeno Takahashi, Hikari Sugawa, Nana Katsuta, Yukio Fujiwara et autres

Adipose tissue plays a central role in metabolic homeostasis, and its dysfunction caused by macrophage-mediated inflammation contributes to vascular disease. This study investigated the role of fumarate in adipocyte–macrophage interactions and its involvement in foam cell formation. Serum levels of S-(2-succinyl)cysteine, a …

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Monoclonal antibody-based assay to identify compounds that inhibit Nδ-(5-hydro-4-imidazolon-2-yl)ornithine (G-H1) formation

Hikari Sugawa, Noriyoshi Manabe, Yoshitaka Hiraoka, Yoshiki Yamaguchi et autres

-(5-hydro-4-imidazolon-2-yl)ornithine (G-H1) accumulates during the progress of atherosclerosis with type 2 diabetes. Therefore, G-H1 might serve as early diagnostic marker of atherosclerosis, and its inhibition could become a strategy to prevent the pathogenesis of this disease. This study therefore aimed to determine …

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Fingertip skin autofluorescence as a non‐invasive marker for vascular complications in patients with type 2 diabetes

Minoru Takeshita, Takeshi Matsumura, Aiko Arimura, Hiroyuki Takahashi et autres

AIMS/INTRODUCTION: This study aimed to investigate whether non-invasive fingertip autofluorescence can evaluate micro- and macrovascular complications in patients with type 2 diabetes. MATERIALS AND METHODS: This multicenter cross-sectional study recruited 452 patients with type 2 diabetes between August 2017 and January 2023. …

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Non-invasive evaluation of advanced glycation end products in hair as early markers of diabetes and aging

Sayuri E. M. Kato, Ryoji Nagai

Continuous metabolic monitoring is essential for assessing lifestyle-related disease risks. Hair, an easily accessible tissue, allows for long-term metabolic evaluation, with glycated proteins linked to diabetic complications found in hair. We established a mass spectrometry system to detect advanced glycation end products …

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Insights from the fructose‐derived product glucoselysine: Revisiting the polyol pathway in diabetic complications

Hiroko Yamaguchi, Ryoji Nagai

Advanced glycation end-products (AGEs) have been extensively studied because of their close association with the onset and progression of diabetic complications. However, owing to their formation through diverse metabolic pathways, AGEs often reflect a wide range of pathological conditions rather than being …

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Rapid and Efficient Synthesis of Succinated Thiol Compounds via Maleic Anhydride Derivatization

Hiroshi Yamaguchi, Hikari Sugawa, Himeno Takahashi, Ryoji Nagai

-(2-succino)cysteine (2SC). While hundreds of 2SC-modified proteins have been identified and are associated with the dysfunction of proteins, the underlying molecular mechanisms remain poorly understood. Conventional methods for synthesizing succinated compounds, such as 2SC, often require prolonged reaction times and/or HCl hydrolysis. …

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Author response for "Rapid formation of Nε-(carboxymethyl)lysine (CML) from ribose depends on glyoxal production by oxidation"

Hikari Sugawa, Tsuyoshi Ikeda, Yuki Tominaga, Nana Katsuta et autres

Stable beam operation of approximately 1 mA beam under highly efficient energy recovery conditions at compact energy-recovery linac

Hiroshi Sakai, Dai Arakawa, T. Furuya, K. Haga et autres

A compact energy-recovery linac (cERL) has been un-der construction at KEK since 2009 to develop key technologies for the energy-recovery linac. The cERL began operating in 2013 to create a high-current beam with a low-emittance beam with stable continuous wave (CW) superconducting …

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Correlation between serum advanced glycation end-products and vascular complications in patient with type 2 diabetes

Sayuri E. M. Kato, Takeshi Matsumura, Hikari Sugawa, Ryoji Nagai

Advanced glycation end-products (AGEs) formation increases with metabolic disorders, leading to higher serum AGE levels in patients with progressive vascular complications. Measuring AGE levels in biological samples requires multiple pre-analytical processing steps, rendering analysis of multiple samples challenging. This study evaluated the …

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Progression of vascular complications enhances the formation of advanced glycation end-products in the serum of patients with type 2 diabetes

Sayuri E. M. Kato, Takeshi Matsumura, Hikari Sugawa, Ryoji Nagai

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Prenylflavonoids isolated from Epimedii Herba show inhibition activity against advanced glycation end-products

Keisuke Nakashima, Hiroyuki Miyashita, Hitoshi Yoshimitsu, Yukio Fujiwara et autres

Introduction: As inhibitors of advanced glycation end products (AGEs), such as pyridoxamine, significantly inhibit the development of retinopathy and neuropathy in rats with streptozotocin-induced diabetes, treatment with AGE inhibitors is believed to be a potential strategy for the prevention of aging, age-related …

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