R. Martin Roop

Silence is golden: How MucR’s repressor activity contributes to Brucella virulence

R. Martin Roop

The small Zn finger protein MucR is an essential virulence determinant in Brucella. This nucleoidassociated protein not only plays a critical role in organizing the structure and topology of the dual chromosomes of these bacteria, but also has global effects on gene …

Breaking the silence—role of MucR as a virulence determinant in Brucella

Ian Scott Barton, Connor B. Cribb, Beatriz Tartilán-Choya, Graham J. Bitzer et autres

ABSTRACT The Zn finger protein MucR is an H-NS-like protein that serves as a gene silencer and nucleoid-structuring protein in the α-proteobacteria. MucR is also an essential virulence determinant in Brucella , where it directly and indirectly controls the expression of many …

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Comparison of CcrM-dependent methylation in Caulobacter crescentus and Brucella abortus by nanopore sequencing

Maxwell Campbell, Ian Scott Barton, R. Martin Roop, Peter C Chien

ABSTRACT Bacteria rely on DNA methylation for restriction-modification systems and epigenetic control of gene expression. Here, we use direct detection of methylated bases by nanopore sequencing to monitor global DNA methylation in Alphaproteobacteria, where use of this technique has not yet been …

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Comparison of CcrM-dependent methylation in Caulobacter crescentus and Brucella abortus by nanopore sequencing

Maxwell Campbell, Ian Scott Barton, R. Martin Roop, Peter C Chien

Abstract Bacteria rely on DNA methylation for restriction-modification systems and epigenetic control of gene expression. Here, we use direct detection of methylated bases by nanopore sequencing to monitor global DNA methylation in Alphaproteobacteria, where use of this technique has not yet been …

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Editorial: Pathogenomics of the genus Brucella and beyond, volume II

Axel Cloeckaert, R. Martin Roop, Holger Christian Scholz, Adrian M. Whatmore et autres

International audience

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Brucella MucR acts as an H-NS-like protein to silence virulence genes and structure the nucleoid

Ian Scott Barton, Zhongqing Ren, Connor B. Cribb, Joshua Edison Pitzer et autres

ABSTRACT Histone-like nucleoid structuring (H-NS) and H-NS-like proteins serve as global gene silencers and work with antagonistic transcriptional activators (counter-silencers) to properly coordinate the expression of virulence genes in pathogenic bacteria. In Brucella , MucR has been proposed as a novel H-NS-like …

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If You're Not Confused, You're Not Paying Attention: Ochrobactrum Is Not Brucella

Edgardo Moreno, Earl A. Middlebrook, Pamela Altamirano-Silva, Sascha Al Dahouk et autres

Bacteria of the genus Brucella are facultative intracellular parasites that cause brucellosis, a severe animal and human disease. Recently, a group of taxonomists merged the brucellae with the primarily free-living, phylogenetically related Ochrobactrum spp. in the genus Brucella .

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A novel formamidase is required for riboflavin biosynthesis in invasive bacteria

Svetlana N. Yurgel, Skylar A. Johnson, Jennifer T. Rice, Na Sa et autres

Biosynthesis of riboflavin (RF), the precursor of the redox cofactors FMN and FAD, was thought to be well understood in bacteria, with all the pathway enzymes presumed to be known and essential. Our previous research has challenged this view by showing that, …

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Characterizing the transport and utilization of the neurotransmitter GABA in the bacterial pathogen Brucella abortus

James A. Budnick, Lauren M. Sheehan, Angela H. Benton, Joshua Edison Pitzer et autres

The neurotransmitter gamma-aminobutyric acid (GABA) is the most abundant inhibitory neurotransmitter in the human brain; however, it is becoming more evident that this non-proteinogenic amino acid plays multiple physiological roles in biology. In the present study, the transport and function of GABA …

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Investigating the roles of the conserved Cu2+-binding residues on Brucella FtrA in producing conformational stability and functionality

Sambuddha Banerjee, Ryan J. Garrigues, Mina N. Chanakira, Jacob J. Negron-Olivo et autres

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The Cation Diffusion Facilitator Family Protein EmfA Confers Resistance to Manganese Toxicity in Brucella abortus 2308 and Is an Essential Virulence Determinant in Mice

Matthew J. Johnsrude, Joshua Edison Pitzer, Daniel W. Martin, R. Martin Roop

Mn nutrition is essential for the basic physiology and virulence ofBrucellastrains. The results of the study presented here demonstrate that the cation diffusion facilitator (CDF)-type metal exporter EmfA plays critical roles in maintaining Mn homeostasis and preventing Mn toxicity inBrucellaand is an …

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That’s the Way You Do It

R. Martin Roop

About one-third of the proteins encoded by the bacterial genomes that have been sequenced to date are proteins of “unknown function.” Studies aimed at defining the biological functions of these proteins represent an important frontier in prokaryotic biology. The study presented by …

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