Jessica M. Skeie

Iron-Sulfur Clusters and Iron Responsive Element Binding Proteins Mediate Iron Accumulation in Corneal Endothelial Cells in Fuchs Dystrophy

Emma M. Hartness, Hanna Shevalye, Jessica M. Skeie, Timothy Eggleston et autres

Purpose: Evidence suggests that corneal endothelial cell (CEC) death in Fuchs endothelial corneal dystrophy (FECD) is due to ferroptosis, an iron-mediated cell death. Iron-sulfur cluster (ISC)-containing aconitases and the iron responsive element binding proteins IREBP1 and IREBP2 are known mediators of iron …

us (code pays fourni par la source)

TCF4 trinucleotide repeat expansions and UV irradiation increase susceptibility to ferroptosis in Fuchs endothelial corneal dystrophy

Sanjib Saha, Jessica M. Skeie, Gregory A. Schmidt, Tim Eggleston et autres

Fuchs endothelial corneal dystrophy (FECD), the leading indication for corneal transplantation in the U.S., causes loss of corneal endothelial cells (CECs) and corneal edema leading to vision loss. FECD pathogenesis is linked to impaired response to oxidative stress and environmental ultraviolet A …

us (code pays fourni par la source)

Rat Model of Type 2 Diabetes Mellitus Recapitulates Human Disease in the Anterior Segment of the Eye

Cheryl L. Wang, Jessica M. Skeie, Chantal Allamargot, Andrew S. Goldstein et autres

Changes in the anterior segment of the eye due to type 2 diabetes mellitus (T2DM) are not well-characterized, in part due to the lack of a reliable animal model. This study evaluated changes in the anterior segment, including crystalline lens health, corneal …

us (code pays fourni par la source)

Squishy matters – Corneal mechanobiology in health and disease

Sara M. Thomasy, Brian C. Leonard, Mark A. Greiner, Jessica M. Skeie et autres

The cornea, as a dynamic and responsive tissue, constantly interacts with mechanical forces in order to maintain its structural integrity, barrier function, transparency and refractive power. Cells within the cornea sense and respond to various mechanical forces that fundamentally regulate their morphology …

us (code pays fourni par la source)

Type II Diabetes Mellitus Causes Extracellular Matrix Alterations in the Posterior Cornea That Increase Graft Thickness and Rigidity

K. Kingsbury, Jessica M. Skeie, Krista Cosert, Gregory A. Schmidt et autres

Purpose: There is a pressing need to investigate the impact of type II diabetes mellitus on the posterior cornea in donor tissues given its increasing prevalence and potential impact on endothelial keratoplasty surgical outcomes. Methods: Immortalized human cultured corneal endothelial cells (CECs; …

us, de (code pays fourni par la source)

Mice Deficient in TAZ (Wwtr1) Demonstrate Clinical Features of Late-Onset Fuchs’ Endothelial Corneal Dystrophy

Brian C. Leonard, Sangwan Park, Soo-Hyun Kim, Laura J. Young et autres

Purpose: We sought to define the role of Wwtr1 in murine ocular structure and function and determine the role of mechanotransduction in Fuchs' endothelial corneal dystrophy (FECD), with emphasis on interactions between corneal endothelial cells (CEnCs) and Descemet's membrane (DM). Methods: A …

us (code pays fourni par la source)

Metabolic and proteomic indications of diabetes progression in human aqueous humor

Christopher R. Fortenbach, Jessica M. Skeie, Kristina Sevcik, A. Tim Johnson et autres

Diabetes mellitus is a multiorgan systemic disease impacting numerous ocular structures that results in significant ocular morbidity and often results in more frequent corneal and glaucoma surgeries for affected individuals. We hypothesize that the systemic metabolic and proteomic derangement observed in the …

us (code pays fourni par la source)

TCF4 trinucleotide repeat expansions and UV irradiation increase susceptibility to ferroptosis in Fuchs endothelial corneal dystrophy

Sanjib Saha, Jessica M. Skeie, Gregory A. Schmidt, Tim Eggleston et autres

ABSTRACT Fuchs endothelial corneal dystrophy (FECD), the leading indication for corneal transplantation in the U.S., causes loss of corneal endothelial cells (CECs) and corneal edema leading to vision loss. FECD pathogenesis is linked to impaired response to oxidative stress and environmental ultraviolet …

us (code pays fourni par la source)

Solubilized Ubiquinol Improves Reactive Oxygen Species Scavenging in Donor Corneal Endothelial Cells

Sanjib Saha, Youssef W. Naguib, Jessica M. Skeie, Timothy M. Acri et autres

us, Égypte (code pays fourni par la source)

Solubilized ubiquinol for preserving corneal function

Youssef W. Naguib, Sanjib Saha, Jessica M. Skeie, Timothy M. Acri et autres

us, Égypte (code pays fourni par la source)

Cell–Matrix Interactions in the Eye: From Cornea to Choroid

Andrew Pouw, Mark A. Greiner, Razek Georges Coussa, Chunhua Jiao et autres

The extracellular matrix (ECM) plays a crucial role in all parts of the eye, from maintaining clarity and hydration of the cornea and vitreous to regulating angiogenesis, intraocular pressure maintenance, and vascular signaling. This review focuses on the interactions of the ECM …

us (code pays fourni par la source)

Mitophagy: An Emerging Target in Ocular Pathology

Jessica M. Skeie, Darryl Nishimura, Cheryl L. Wang, Gregory A. Schmidt et autres

Mitochondrial function is essential for the viability of aerobic eukaryotic cells, as mitochondria provide energy through the generation of adenosine triphosphate (ATP), regulate cellular metabolism, provide redox balancing, participate in immune signaling, and can initiate apoptosis. Mitochondria are dynamic organelles that participate …

us (code pays fourni par la source)