Juan Fandiño

Proinflammatory Cytokine Preconditioning Enhances the Therapeutic Potency of Different Types of MSCs in Inflammation

Lanzhi Liu, Juan Fandiño, Abigail J. M. Warren, Rui Shi et autres

Mesenchymal stromal cells (MSCs) have shown immunomodulatory effects and great promise in many inflammatory diseases such as acute respiratory distress syndrome (ARDS). However, several barriers to translation remain such as cell availability and potency. This study evaluates the therapeutic potentials of three …

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An evaluation of Tie2 mRNA as a potential therapeutic for ventilator-induced lung injury

Shanshan Du, Sean McCarthy, Claire Masterson, Juan Fandiño et autres

Despite the necessary, lifesaving use of mechanical ventilation in critical care settings, it can often contribute to further lung injury in ARDS patients. Pulmonary inflammation and vascular leakage are hallmarks of ARDS. Recent studies suggest that endothelial dysfunction in pulmonary infection and …

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Study of the in vitro therapeutic efficacy of preconditioned MSCs for the treatment of ARDS

Lanzhi Liu, Juan Fandiño, Claire Masterson, Shanshan Du et autres

Rationale: Mesenchymal stem cells (MSCs) have demonstrated therapeutic potential in ARDS, but this has not fully translated to efficacy in clinical trials. The most efficient cell type and pre-activation method have yet to be determined. We performed in vitro potency testing by …

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The Effects of the Pneumonia Lung Microenvironment on MSC Function

Lanzhi Liu, Juan Fandiño, Sean McCarthy, Claire Masterson et autres

Background: Despite promise in preclinical models of acute respiratory distress syndrome (ARDS), mesenchymal stem cells (MSC) have failed to translate to therapeutic benefit in clinical trials. The MSC is a live cell medicine and interacts with the patient’s disease state. Here, we …

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Cytokine preconditioning enhances the therapeutic potential of MSCs in ARDS

Lanzhi Liu, Juan Fandiño, Claire Masterson, John G. Laffey et autres

Rationale: Mesenchymal stem cell (MSC) therapy for acute respiratory distress syndrome (ARDS) has shown safety in clinical trials but efficacy remains unproven. Thus, MSC preconditioning has been proposed as a strategy to increase MSC efficacy. This project aims to explore the therapeutic …

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MSCs are protective against ‘second hit’ injury during pulmonary sepsis in mechanically ventilated rodents.

Claire Masterson, Ignacio Sallent, Lanzhi Liu, Sean Mc Carthy et autres

Introduction: Pulmonary sepsis is a leading cause of ARDS and death in critically ill patients. Critically ill sepsis patients are at risk of secondary infection due to acquired immunosuppression after prolonged infection [Hotchkiss, 2013]. Here, we aimed to show that in a …

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Therapeutic application of extracellular vesicle-encapsulated CC16 in acute lung injury

Lanzhi Liu, Juan Fandiño, Daniel O’Toole

Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a critical clinical syndrome spectrum with high incidence and associated mortality. It is characterized by acute hypoxemia, reduced lung compliance, and non-cardiogenic pulmonary edema, which lead to respiratory failure.1,2 Despite considerable progress in …

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Aerosolized Pulmonary Delivery of mRNA Constructs Attenuates Severity of Escherichia coli Pneumonia in the Rat

Sean McCarthy, Christopher B. Rohde, Matt Angel, Claire Masterson et autres

Acute respiratory distress syndrome (ARDS), a rapid onset inflammatory lung disease with no effective specific therapy, typically has pathogenic etiology termed pneumonia. In previous studies nuclear factor-κB (NF-κB) inhibitor α super-repressor (IκBα-SR) and extracellular superoxide dismutase 3 (SOD3) reduced pneumonia severity when …

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Differential Effects of Cytokine Versus Hypoxic Preconditioning of Human Mesenchymal Stromal Cells in Pulmonary Sepsis Induced by Antimicrobial-Resistant Klebsiella pneumoniae

Declan Byrnes, Claire Masterson, Jack Brady, Senthilkumar Alagesan et autres

Background: Pulmonary sepsis is a leading cause of hospital mortality, and sepses arising from antimicrobial-resistant (AMR) bacterial strains are particularly difficult to treat. Here we investigated the potential of mesenchymal stromal cells (MSCs) to combat established Klebsiella pneumoniae pneumosepsis and further evaluated …

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Enhancement strategies for mesenchymal stem cells and related therapies

Senthilkumar Alagesan, Jack Brady, Declan Byrnes, Juan Fandiño et autres

Cell therapy, particularly mesenchymal stem/stromal (MSC) therapy, has been investigated for a wide variety of disease indications, particularly those with inflammatory pathologies. However, recently it has become evident that the MSC is far from a panacea. In this review we will look …

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Liraglutida, agonista del receptor de glp-1, regula la expresión de los componentes hepáticos de ras en crías de ratas sometidas perinatalmente a restricción calórica.

Zainab Mastoor, Juan Fandiño, Laura Toba, Lucas C. González-Matías et autres

Hypercapnia in the critically ill: insights from the bench to the bedside

Claire Masterson, Shahd Horie, Sean McCarthy, Declan Byrnes et autres

Carbon dioxide (CO 2 ) has long been considered, at best, a waste by-product of metabolism, and at worst, a toxic molecule with serious health consequences if physiological concentration is dysregulated. However, clinical observations have revealed that ‘permissive’ hypercapnia, the deliberate allowance …

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