Yakov A. Lomakin

Mining antibody functionality via AI-guided structural landscape profiling

Stanislav S. Terekhov, Nikita V. Ivanisenko, Nan Zhang, Yuliana A. Mokrushina et autres

Despite substantial progress in single-cell screening techniques, antibody (Ab) repertoires still remain enigmatic. Here we show that Ab sequences can be linked to their functionality by using big data obtained from high-throughput sequencing. Using the expansive SARS-CoV-2 pandemic data, we develop an …

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Extracellular Vesicles as a Source of Biomarkers for Cancer Diagnosis

Leyla A. Ovchinnikova, Yakov A. Lomakin

Extracellular vesicles (EVs) are secreted by nearly all mammalian cells and play a major role in intercellular communication via the transport of various active biomolecules. In cancer, pathological EVs contribute to tumor progression by participating in metastasis, angiogenesis, and immune evasion. Recent …

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Assessment of Human Ig Repertoire Diversity after Cryopreservation and Restimulation of B Cells

Anastasia Smirnova, Leyla A. Ovchinnikova, Ilgar Z. Mamedov, Tatiana V. Grigoreva et autres

Cryopreservation of human B cells is widely employed in clinical and research applications. However, it is still commonly believed that only freshly isolated B cells should be analyzed to accurately evaluate, both quantitatively and qualitatively, the immunoglobulin (Ig) repertoire. In this study, …

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Production of Genetically Engineered Extracellular Vesicles for Targeted Protein Delivery

Leyla A. Ovchinnikova, Evgeniy G. Evtushenko, Dmitry V. Bagrov, Alexander G. Gabibov et autres

Extracellular vesicles (EVs) have emerged as promising carriers for the targeted delivery of therapeutic proteins to specific cells. Previously, we demonstrated that genetically engineered EVs can be used for targeted protein delivery. This protocol details the generation of mannose receptor (CD206)-targeted EVs …

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Development of a panel of biomarkers for differential diagnosis of multiple sclerosis

Leyla A. Ovchinnikova, Samir S. Dzhelad, Taras O. Simaniv, M. N. Zakharova et autres

Demyelinating diseases are a group of heterogeneous pathologies that affect the nervous system and reduce the quality of life. One such disease is multiple sclerosis (MS), an inflammatory autoimmune neurodegenerative disease of the central nervous system (CNS). At the initial stages, MS …

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Targeted macrophage mannose receptor (CD206)-specific protein delivery via engineered extracellular vesicles

Leyla A. Ovchinnikova, Daria Yu. Tanygina, Samir S. Dzhelad, Evgeniy G. Evtushenko et autres

cells. Our study outlines an optimized platform for the production of targeted EVs designed for specific protein delivery to CD206-positive human cells.

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High heterogeneity of cross-reactive immunoglobulins in multiple sclerosis presumes combining of B-cell epitopes for diagnostics: a case-control study

Leyla A. Ovchinnikova, Igor E. Eliseev, Samir S. Dzhelad, Taras O. Simaniv et autres

Background: Multiple sclerosis (MS) is a neuroinflammatory disease triggered by a combination of genetic traits and external factors. Autoimmune nature of MS is proven by the identification of pathogenic T cells, but the role of autoantibody-producing B cells is less clear. A …

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Autoantigenic peptide landscape of rheumatoid arthritis-associated HLA class II

Irina A. Ishina, Anton Zhiyanov, Inna N. Kurbatskaia, Azad E. Mamedov et autres

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Development of a Panel of Biomarkers for Differential Diagnosis of Multiple Sclerosis

Leyla A. Ovchinnikova, Samir S. Dzhelad, Taras O. Simaniv, Mariya N. Zakharova et autres

Abstract Demyelinating diseases are a group of heterogeneous pathologies that affect the nervous system and reduce the quality of life. One of such diseases is multiple sclerosis (MS), an inflammatory autoimmune neurodegenerative disease of the central nervous system (CNS). At the initial …

ru (code pays fourni par la source)

Two-dimensional high-throughput on-cell screening of immunoglobulins against broad antigen repertoires

Yakov A. Lomakin, Leyla A. Ovchinnikova, Stanislav S. Terekhov, Samir S. Dzhelad et autres

Identifying high-affinity antibodies in human serum is challenging due to extremely low number of circulating B cells specific to the desired antigens. Delays caused by a lack of information on the immunogenic proteins of viral origin hamper the development of therapeutic antibodies. …

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The level of anti-viral antigen-specific antibodies to EBNA-1 in the serum of ms patients does not depend on the severity of the disease

Leyla A. Ovchinnikova, Samir S. Dzhelad, Taras O. Simaniv, Mariya N. Zakharova et autres

Multiple sclerosis (MS) is an autoimmune neurodegenerative disease leading to inevitable disability and primarily affecting the young and middle-aged population. Recent studies have shown a direct correlation between the risk of MS development and Epstein-Barr virus (EBV) infection. Analysis of the titer …

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The Level of Anti-Viral Antigen-Specific Antibodies to EBNA-1 in the Serum of MS Patients Does not Depend on the Severity of the Disease

Leyla A. Ovchinnikova, Samir S. Dzhelad, Taras O. Simaniv, Mariya N. Zakharova et autres

Multiple sclerosis (MS) is an autoimmune neurodegenerative disease leading to inevitable disability and primarily affecting the young and middle-aged population. Recent studies have shown a direct correlation between the risk of MS development and Epstein-Barr virus (EBV) infection. Analysis of the titer …

ru (code pays fourni par la source)