Jakob M. Silberberg

A two-step mechanism for sugar translocation

Do-Hwan Ahn, Claudia Alleva, Tom Reichenbach, Ashutosh Gulati et autres

In mammals, glucose transporters (GLUTs) mediate organism-wide sugar distribution, yet the molecular basis of substrate specificity remains unclear. The bacterial xylose transporter XylE serves as a model for GLUTs. However, although xylose and glucose bind with a similar affinity, xylose is transported, …

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KdpD is a tandem serine histidine kinase that controls K+ pump KdpFABC transcriptionally and post-translationally

Jakob M. Silberberg, Sophie Ketter, P. Böhm, Kristin Jordan et autres

Two-component systems, consisting of a histidine kinase and a response regulator, serve signal transduction in bacteria, often regulating transcription in response to environmental stimuli. Here, we identify a tandem serine histidine kinase function for KdpD, previously described as a histidine kinase of …

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Sensory kinase KdpD is a tandem serine histidine kinase controlling K + pump KdpFABC on the translational and post-transcriptional level

Jakob M. Silberberg, Sophie Ketter, P. Böhm, Kristin Jordan et autres

Abstract Two-component systems (TCSs), consisting of a histidine kinase (HK) and a response regulator, serve signal transduction in bacteria, often regulating transcription in response to environmental stimuli. Here, we identify a tandem serine histidine kinase function for KdpD, previously described as a …

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Inhibited KdpFABC transitions into an E1 off-cycle state

Jakob M. Silberberg, Charlott Stock, Lisa Hielkema, Robin A. Corey et autres

KdpFABC is a high-affinity prokaryotic K + uptake system that forms a functional chimera between a channel-like subunit (KdpA) and a P-type ATPase (KdpB). At high K + levels, KdpFABC needs to be inhibited to prevent excessive K + accumulation to the …

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Author response: Inhibited KdpFABC transitions into an E1 off-cycle state

Jakob M. Silberberg, Charlott Stock, Lisa Hielkema, Robin A. Corey et autres

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Protein diffusion in Escherichia coli cytoplasm scales with the mass of the complexes and is location dependent

Wojciech M. Śmigiel, Luca Mantovanelli, Dmitrii Linnik, Christiaan M. Punter et autres

We analyze the structure of the cytoplasm by performing single-molecule displacement mapping on a diverse set of native cytoplasmic proteins in exponentially growing Escherichia coli . We evaluate the method for application in small compartments and find that confining effects of the …

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Inhibited KdpFABC resides in an E1 off-cycle state

Jakob M. Silberberg, Charlott Stock, Lisa Hielkema, Robin A. Corey et autres

Abstract KdpFABC is a high-affinity prokaryotic K + uptake system that forms a functional chimera between a channel-like subunit (KdpA) and a P-type ATPase (KdpB). At high K + levels, KdpFABC needs to be inhibited to prevent excessive K + accumulation to …

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Wie Bakterien die Aufnahme von Kaliumionen regulieren

Jakob M. Silberberg, Inga Hänelt

Abstract K+ plays an essential role in a different cellular processes in bacteria, and is a central player in microbial adaptation towards a number of environmental challenges. Accordingly, K+ transporters are subject to tight regulation by a diverse set of mechanisms. Here, …

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Deciphering ion transport and ATPase coupling in the intersubunit tunnel of KdpFABC

Jakob M. Silberberg, Robin A. Corey, Lisa Hielkema, Charlott Stock et autres

Abstract KdpFABC, a high-affinity K + pump, combines the ion channel KdpA and the P-type ATPase KdpB to secure survival at K + limitation. Here, we apply a combination of cryo-EM, biochemical assays, and MD simulations to illuminate the mechanisms underlying transport …

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Deciphering ion transport and ATPase coupling in the intersubunit tunnel of KdpFABC

Jakob M. Silberberg, Robin A. Corey, Lisa Hielkema, Charlott Stock et autres

Abstract KdpFABC, a high-affinity K + pump, combines the ion channel KdpA and the P-type ATPase KdpB to secure survival at K + limitation. Here, we apply a combination of cryo-EM, biochemical assays, and MD simulations to illuminate the mechanisms underlying transport …

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Molecular Mechanisms for Bacterial Potassium Homeostasis

Janina Stautz, Yvonne Hellmich, Michael F. Fuss, Jakob M. Silberberg et autres

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Cryo-EM structure of the KdpFABC complex in an E1-ATP conformation loaded with K+

Jakob M. Silberberg, Robin A. Corey, Lisa Hielkema, Christopher Stock et autres