Lu-Jun Liang

An Integrated Solubilizing-Tag-Free Strategy for Chemical Synthesis of Mirror-Image Interleukin-6

Yuxiang Ren, Yanbo Liu, Dongyang Han, Lu-Jun Liang

Interleukin-6 (IL-6) stands as a therapeutically important target, and the efficient chemical synthesis of mirror-image D-IL-6 could facilitate the development of D-peptide antagonists to complement the existing repertoire of antibody-based antagonists. Herein, we report a new integrated synthetic strategy for D-IL-6 characterized …

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Design and Semisynthesis of Ubiquitin Extension Probes for Structural Analysis of Cullin1-Mediated Substrate Polyubiquitination

Chuntong Li, Chong Zuo, Liying Zhang, Yangwode Jing et autres

Chemical trapping strategies have recently emerged as powerful approaches for investigating the structural dynamics of E3 ligase-catalyzed substrate ubiquitination. However, current ubiquitination-derived probes are limited to studying substrate mono- or diubiquitination events. Probes capable of investigating how E3 ligases accommodate E2–Ub conjugates …

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Synthesis of an E2-Ub-nucleosome conjugate to capture the E3 ligase PHF7-catalyzed H3K14 ubiquitination intermediate

Shixian Tao, Wei He, Chuntong Li, Zebin Tong et autres

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Superfast Protein Desulfurization Triggered by Low‐Energy Visible Light

Dongyang Han, Xiangyu Deng, Yan Cui, Xinyue Zhu et autres

The combination of transthioesterification-based ligation of thiol-derived amino acids and the post-ligation desulfurization has greatly expanded the scope of modern chemical protein synthesis. Here, we report a new strategy of low-energy visible light-induced desulfurization (LEnVLD) that enables superfast and clean protein desulfurization …

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Mechanically Triggered Protein Desulfurization

Dongyang Han, Yan Cui, Xiangyu Deng, Chuntong Li et autres

The technology of native chemical ligation and postligation desulfurization has greatly expanded the scope of modern chemical protein synthesis. Here, we report that ultrasonic energy can trigger robust and clean protein desulfurization, and we developed an ultrasound-induced desulfurization (USID) strategy that is …

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Structure-guided engineering enables E3 ligase-free and versatile protein ubiquitination via UBE2E1

Xiangwei Wu, Yunxiang Du, Lu-Jun Liang, Ruichao Ding et autres

Ubiquitination, catalyzed usually by a three-enzyme cascade (E1, E2, E3), regulates various eukaryotic cellular processes. E3 ligases are the most critical components of this catalytic cascade, determining both substrate specificity and polyubiquitination linkage specificity. Here, we reveal the mechanism of a naturally …

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WCN23-0790 Ubiquitination in the Proteome in Mouse Model of Diabetic Nephropathy

Lu-Jun Liang, Mingyu He, Sixuan Pan, S. Zhou et autres