Mechanically Triggered Protein Desulfurization
Rattachement africain : cn, ch, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
The technology of native chemical ligation and postligation desulfurization has greatly expanded the scope of modern chemical protein synthesis. Here, we report that ultrasonic energy can trigger robust and clean protein desulfurization, and we developed an ultrasound-induced desulfurization (USID) strategy that is simple to use and generally applicable to peptides and proteins. The USID strategy involves a simple ultrasonic cleaning bath and an easy-to-use and easy-to-remove sonosensitizer, titanium dioxide. It features mild and convenient reaction conditions and excellent functional group compatibility, e.g., with thiazolidine (Thz) and serotonin, which are sensitive to other desulfurization strategies. The USID strategy is robust: without reoptimizing the reaction conditions, the same USID procedure can be used for the clean desulfurization of a broad range of proteins with one or more sulfhydryl groups, even in multi-hundred-milligram scale reactions. The utility of USID was demonstrated by the one-pot synthesis of bioactive cyclopeptides such as Cycloleonuripeptide E and Segetalin F, as well as convergent chemical synthesis of functionally important proteins such as histone H3.5 using Thz as a temporary protecting group. A mechanistic investigation indicated that USID proceeds via a radical-based mechanism promoted by low-frequency and low-intensity ultrasonication. Overall, our work introduces a mechanically triggered approach with the potential to become a robust desulfurization method for general use in chemical protein synthesis by both academic and industrial laboratories.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Mechanically Triggered Protein Desulfurization
- Date Crossref
- 24/01/2025
- Éditeur
- American Chemical Society (ACS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Center for Life Sciences pays non établi dans la noticeUniversité ou école supérieure
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Tsinghua University New Cornerstone Science Laboratory pays non établi dans la noticeUniversité ou école supérieure
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University of Science and Technology of China Hefei National Laboratory of Physical Science at Microscale pays non établi dans la noticeUniversité ou école supérieure
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Bioanalytica (Switzerland) pays non établi dans la noticeEntreprise
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Hefei National Center for Physical Sciences at Nanoscale pays non établi dans la noticeStructure de recherche
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University of Miami Desai Sethi Urology Institute pays non établi dans la noticeUniversité ou école supérieure
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Sylvester Comprehensive Cancer Center pays non établi dans la noticeÉtablissement de santé
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New Cornerstone Science Laboratory pays non établi dans la noticeStructure de recherche
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Hefei National Laboratory of Physical Science at Microscale Center for BioAnalytical Chemistry pays non établi dans la noticeStructure de recherche
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The First Affiliated Hospital of USTC Department of Oncology pays non établi dans la noticeÉtablissement de santé
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Desai Sethi Urology Institute pays non établi dans la noticeStructure de recherche
Center for Life Sciences, New Cornerstone Science Laboratory — Tsinghua University et Hefei National Laboratory of Physical Science at Microscale — University of Science and Technology of China, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.