Paul Stolz

Spatiotemporal regulation of LGN/NuMA and astral microtubules generate mirror symmetric spindle rotations

Janet Chenevert, Anne Rosfelter, Daniel González-Suárez, Silvia Caballero-Mancebo et autres

Abstract The positioning of the mitotic spindle controls the size, content, and position of daughter cells within embryos and tissues. A major spindle positioning mechanism is “cortical pulling” whereby the membrane-bound complex LGN/NuMA/Dynein captures astral microtubules and pulls centrosomes toward the cell …

fr, jp (code pays fourni par la source)

TET1 regulates gene expression and repression of endogenous retroviruses independent of DNA demethylation

Paul Stolz, Angelo Salazar Mantero, Andrey Tvardovskiy, Enes Ugur et autres

DNA methylation (5-methylcytosine (5mC)) is critical for genome stability and transcriptional regulation in mammals. The discovery that ten-eleven translocation (TET) proteins catalyze the oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) revolutionized our perspective on the complexity and regulation …

de, se (code pays fourni par la source)

Author Correction: Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals

Christopher B. Mulholland, Atsuya Nishiyama, Joël Ryan, Ryohei Nakamura et autres

A Correction to this paper has been published: https://doi.org/10.1038/s41467-020-20453-0

de, jp, gb (code pays fourni par la source)

Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals

Christopher B. Mulholland, Atsuya Nishiyama, Joël Ryan, Ryohei Nakamura et autres

Genome-wide DNA demethylation is a unique feature of mammalian development and naïve pluripotent stem cells. Here, we describe a recently evolved pathway in which global hypomethylation is achieved by the coupling of active and passive demethylation. TET activity is required, albeit indirectly, …

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METTL6 is a tRNA m 3 C methyltransferase that regulates pluripotency and tumor cell growth

Valentina V. Ignatova, Steffen Kaiser, Jessica Ho, Xinyang Bing et autres

knockout results in reduced energy expenditure. We reveal a previously unknown pathway in the maintenance of translation efficiency with a role in maintaining stem cell self-renewal, as well as impacting tumor cell growth profoundly.

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Distinct and stage-specific contributions of TET1 and TET2 to stepwise cytosine oxidation in the transition from naive to primed pluripotency

Christopher B. Mulholland, Franziska R. Traube, Enes Ugur, Edris Parsa et autres

Cytosine DNA bases can be methylated by DNA methyltransferases and subsequently oxidized by TET proteins. The resulting 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) are considered demethylation intermediates as well as stable epigenetic marks. To dissect the contributions of these cytosine modifying …

de, gb (code pays fourni par la source)

The rRNA m 6 A methyltransferase METTL5 is involved in pluripotency and developmental programs

Valentina V. Ignatova, Paul Stolz, Steffen Kaiser, Tobias Gustafsson et autres

Covalent chemical modifications of cellular RNAs directly impact all biological processes. However, our mechanistic understanding of the enzymes catalyzing these modifications, their substrates and biological functions, remains vague. Amongst RNA modifications N 6 -methyladenosine (m 6 A) is widespread and found in …

de, us, nl (code pays fourni par la source)

Critical Role of the UBL Domain in Stimulating the E3 Ubiquitin Ligase Activity of UHRF1 toward Chromatin

Benjamin M. Foster, Paul Stolz, Christopher B. Mulholland, Alex Montoya et autres

The RING E3 ubiquitin ligase UHRF1 controls DNA methylation through its ability to target the maintenance DNA methyltransferase DNMT1 to newly replicated chromatin. DNMT1 recruitment relies on ubiquitylation of histone H3 by UHRF1; however, how UHRF1 deposits ubiquitin onto the histone is …

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Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive demethylation in mammals

Christopher B. Mulholland, Atsuya Nishiyama, Joël Ryan, Ryohei Nakamura et autres

Abstract Genome-wide DNA demethylation is a unique feature of mammalian development and naïve pluripotent stem cells. So far, it was unclear how mammals specifically achieve global DNA hypomethylation, given the high conservation of the DNA (de-)methylation machinery among vertebrates. We found that …

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Distinct and stage-specific contributions of TET1 and TET2 to stepwise cytosine oxidation in the transition from naive to primed pluripotency

Christopher B. Mulholland, Franziska R. Traube, Edris Parsa, Eva-Maria Eckl et autres

ABSTRACT The TET-oxidized cytosine derivatives, 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC), are considered DNA demethylation intermediates as well as stable epigenetic marks in mammals. We compared modified cytosine and enzyme levels in TET-knockout cells during naive pluripotency exit and found distinct and differentiation-dependent …

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The ERN 1 transcription factor gene is a target of the CC a MK / CYCLOPS complex and controls rhizobial infection in Lotus japonicus

Marion R. Cerri, Quanhui Wang, Paul Stolz, Jessica Folgmann et autres

Bacterial accommodation inside living plant cells is restricted to the nitrogen-fixing root nodule symbiosis. In many legumes, bacterial uptake is mediated via tubular structures called infection threads (ITs). To identify plant genes required for successful symbiotic infection, we screened an ethyl methanesulfonate …

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Production of the Tissue Inhibitor of Metalloproteinase‐2 (TIMP‐2) and Evaluation of Its Potential as Anti‐Aging Active

R. Schütz, Bettina König, B. Hochstrasser, Paul Stolz et autres

Collagen‐degrading matrix metalloproteinases (MMPs) show increased activities predominantly in UV‐irradiated skin as well as in chronologically aged skin. MMPs are therefore reasoned to be associated with the degenerative alterations in the collagenous dermal extracellular matrix, which in turn may accelerate the signs …