Antonio Artigues

Proteomics Used in Identifying Novel Correlates of Disease in Pediatric Metabolic Dysfunction‐Associated Steatotic Liver Disease

Diego Paine Cabrera, Lisa K. Harvey, Michele T. Pritchard, John P. Thyfault et autres

Metabolic dysfunction-associated liver disease (MASLD) is a leading cause of liver disease in children. There is a paucity of data on potential biomarkers and therapeutic targets, especially in pediatric MASLD. We used mass spectrometry (MS)-mediated proteomics followed by enzyme-linked immunosorbent assay (ELISA) …

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Multi-omics after O-GlcNAc alteration identified cellular processes promoting aneuploidy after loss of O-GlcNAc transferase

Samuel S. Boyd, Dakota R. Robarts, Khue P. Nguyen, Maite Villar et autres

OBJECTIVE: Pharmacologic or genetic manipulation of O-GlcNAcylation, an intracellular, single sugar post-translational modification, are difficult to interpret due to the pleotropic nature of O-GlcNAc and the vast signaling pathways it regulates. METHOD: To address the pleotropic nature of O-GlcNAc, we employed either …

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Site-specific O-GlcNAcylation of progesterone receptor (PR) supports PR attenuation of interferon stimulated genes (ISGs) and tumor growth in breast cancer

Harmony Saunders, Sean M. Holloran, Gloria M. Trinca, Antonio Artigues et autres

Hormone receptor positive (HR+) breast cancer, defined by expression of estrogen receptor (ER) and/or progesterone receptor (PR), is the most commonly diagnosed type of breast cancer. PR alters the transcriptional landscape to support tumor growth in concert with, or independent of, ER. …

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Multi-Omics after O-GlcNAc Alteration Identifies Cellular Processes Working Synergistically to Promote Aneuploidy

Samuel S. Boyd, Dakota R. Robarts, Khue P. Nguyen, Maite Villar et autres

Pharmacologic or genetic manipulation of O-GlcNAcylation, an intracellular, single sugar post-translational modification, are difficult to interpret due to the pleotropic nature of O-GlcNAc and the vast signaling pathways it regulates. To address this issue, we employed either OGT (O-GlcNAc transferase), OGA (O-GlcNAcase) …

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Abstract 1852 Downregulation of the nutrient-sensitive post-translational modification, O-GlcNAcylation, attenuates Autosomal Dominant Polycystic Kidney Disease

Matthew A. Kavanaugh, Dona Greta Isai, Vincent W. T. Lam, Maria Villar-Lecumberri et autres

The ability to inactivate gene function is essential for understanding normal development and the development of disease.The Latching Orthogonal Cage and Key pRoteins (LOCKR) technology uses orthogonal Switches and Keys to control gene expression at the protein level.The Switch functions to cage …

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Abstract 2007 Evaluation of the changes in proteome and phosphome upon O-GlcNAc mediated disruption of ERK signaling

Sophiya John Ephrame, Antonio Artigues, Maria Villar-Lecumberri, Jenna Shawa et autres

Pharmacological and genetic disruptions to O-GlcNAc increase ERK activation, which is involved in the pathogenesis of several diseases like Alzheimer's disease. O-GlcNAc is a single sugar post-translational modification that dynamically cycles on and off proteins in response to nutrient changes by the …

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Abstract 2150 Using System Biology Approaches to Understand the O-GlcNAc Rheostat

Khue P. Nguyen, Samuel S. Boyd, Dakota R. Robarts, Manasi Kotulkar et autres

O-GlcNAcylation is a post-translational modification that plays an essential role in protein function, stability, localization, and interaction. However, ubiquitous and pleiotropic nature of O-GlcNAc makes understanding the complex role of this modification ambiguous. O-GlcNAc is processed by two key enzymes: a single …

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Abstract 2028 Long-term O-GlcNAc elevation effects brain mitochondrial activity and reactive oxygen species production

Ibtihal M. Alghusen, Marisa S. Carman, Heather Wilkins, Maria Villar-Lecumberri et autres

Mitochondria are dynamic organelles that play essential roles in key metabolic activities from energy production to cellular signaling. Mitochondrial adaptation to stress and metabolic demands relies on a range of reversible post translation modifications (PTMs). These modifications are linked to apoptosis, oxidative …

Translocation of Adenosine A2B Receptor to Mitochondria Influences Cytochrome P450 2E1 Activity after Acetaminophen Overdose

Giselle Sánchez-Guerrero, David S. Umbaugh, Abhay A. Ramachandran, Antonio Artigues et autres

The adenosine A2B receptor (A2BAR) is a member of a family of G-protein coupled receptors (GPCRs), which has a low affinity for adenosine and is now implicated in several pathophysiological conditions. We have demonstrated the beneficial effects of A2BAR activation in enhancing …

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Downregulation of O-GlcNAcylation, a Metabolic Regulator, Attenuates PKD Progression

Matthew A. Kavanaugh, Dona Greta Isai, Henry Wang, Antonio Artigues et autres

Background: Altered cell metabolism is an important component of autosomal dominant polycystic kidney disease (ADPKD) pathogenesis, but drivers of these alterations are not understood. The addition of O-linked b-N-acetylglucosamine (O-GlcNAc) onto protein substrates by O-GlcNAc transferase (OGT) is a nutrient-sensitive post-translational modification …

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Abstract 2527: Workflow for the reproducible identification and quantification of O-GlcNAcylation in complex proteomes

Antonio Artigues, Maria Villar-Lecumberri, Wagmer Dias, Benyi Li et autres

The structure of caseinolytic protease subunit ClpP2 reveals a functional model of the caseinolytic protease system from Chlamydia trachomatis

Jahaun Azadmanesh, Mohamed A. Seleem, Lucas R. Struble, Nicholas A. Wood et autres

Chlamydia trachomatis (ct) is the most reported bacterial sexually transmitted infection worldwide and the leading cause of preventable blindness. Caseinolytic proteases (ClpP) from pathogenic bacteria are attractive antibiotic targets, particularly for bacterial species that form persister colonies with phenotypic resistance against common …

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