Barbara A. Schmitt

Abstract 183: High-content screening reveals kinase regulators of Dvl2 phase separation in Wnt signaling: Implications for targeted cancer therapy

Antonia Schubert, Florian Heigwer, Christian Scheeder, Oksana Voloshanenko et autres

Abstract Wnt signaling controls cell proliferation, differentiation, and migration, with critical roles in diseases such as cancer. It was proposed that Wnt signaling relies on the formation of dynamic biomolecular condensates, such as the “signalosome” and the “destruction complex”, with a potential …

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Image-based screens identify regulators of endogenous Dvl2 biomolecular condensates

Antonia Schubert, Florian Heigwer, Christian Scheeder, Oksana Voloshanenko et autres

Summary Dishevelled (Dvl) proteins are essential transducers in Wnt signaling pathways, which have been implicated in development, stem cell maintenance, and human diseases such as cancer. Several studies have shown that Dvl proteins form dynamic biomolecular condensates. However, how cellular signals and …

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Assessing In‐Vitro Anti‐diabetic and Anti‐Browning Properties of Polyphenols Extracted from Hydrangea machrophylla Leaves

Ahlam Al-Yafeai, Barbara A. Schmitt, Angelika Malarski, Volker Böhm

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Restraining Wnt activation and intestinal tumorigenesis by a Rab35 dependent GTPase relay

Siamak Redhai, Tianyu Wang, Kim E. Boonekamp, Saskia Reuter et autres

ABSTRACT Maintenance of homeostatic processes ensure curtailment of intestinal tumorigenesis. Inactivating mutations to Adenomatous Polyposis Coli ( Apc ) result in aberrantly activated Wnt signalling and initiates colorectal cancer (CRC) in approx. 80% of cases, yet our understanding of the subcellular mechanisms …

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In-Vitro Bioactivity Evaluation of Hydrangenol Extracted from Hydrangea macrophylla (Thunb.) Ser. Leaves

Ahlam Al-Yafeai, Barbara A. Schmitt, Angelika Malarski, Volker Böhm

Hydrangea macrophylla plant, native to Japan and Korea, has been attracting scientific attention due to its potential applications in both food science and health-related research. In this investigation, dry Hydrangea leaves were utilized as the source material. Subsequent to comminution and thermal …

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P24-39: New data supporting recognition of evident toxicity in acute oral toxicity studies (OECD TG 420)

Fiona R. Sewell, D. Andrew, T. Holmes, G. Horgan et autres

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A global genetic interaction network by single-cell imaging and machine learning

Florian Heigwer, Christian Scheeder, Josephine Bageritz, Schayan Yousefian et autres

Cellular and organismal phenotypes are controlled by complex gene regulatory networks. However, reference maps of gene function are still scarce across different organisms. Here, we generated synthetic genetic interaction and cell morphology profiles of more than 6,800 genes in cultured Drosophila cells. …

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SUTURA ELÁSTICA: UMA OPÇÃO NO TRATAMENTO DE ESCALPE – RELATO DE CASO

Barbara A. Schmitt, Daniela Pavin, Lucas Cunha de Andrade

Introduction: extensive scalp injuries are a challenge in plastic surgery, especially in the context of trauma. The management of these injuries has aesthetic, social and psychological impact and is important to prevent infections. Surgical treatment options include grafts, local and free flaps, …

The Evolution of Ecological Diversity in Acidobacteria

Johannes Sikorski, Vanessa Baumgartner, Klaus Birkhofer, Runa S. Boeddinghaus et autres

Acidobacteria occur in a large variety of ecosystems worldwide and are particularly abundant and highly diverse in soils. In spite of their diversity, only few species have been characterized to date which makes Acidobacteria one of the most poorly understood phyla among …

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Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library

Luisa Henkel, Benedikt Rauscher, Barbara A. Schmitt, Jan G. de Winter et autres

BACKGROUND: In recent years, large-scale genetic screens using the CRISPR/Cas9 system have emerged as scalable approaches able to interrogate gene function with unprecedented efficiency and specificity in various biological contexts. By this means, functional dependencies on both the protein-coding and noncoding genome …

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Pooled CRISPR screening at high sensitivity with an empirically designed sgRNA library

Luisa Henkel, Benedikt Rauscher, Barbara A. Schmitt, Jan G. de Winter et autres

ABSTRACT Given their broad utility in functionally annotating genomes, the experimental design of genome-scale CRISPR screens can vary greatly and criteria for optimal experimental implementation and library composition are still emerging. In this study, we report advantages of conducting viability screens in …

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Additional file 4 of Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library

Luisa Henkel, Benedikt Rauscher, Barbara A. Schmitt, Jan G. de Winter et autres

Additional file 4: File S2. sgRNA sequences of the HD CRISPR Library B