Yoko Oyama

AMPK/p38 MAPK signaling selectively enhances HIF-induced VEGF-A165 expression under hypoxic and low-glucose conditions in HepG2 cells to promote endothelial cell proliferation and migration

Hirohito Hashinokuchi, Munekazu Yamakuchi, Sadayuki Higashi, Kazunori Takenouchi et autres

BACKGROUND: Angiogenesis is essential for tumor development and growth. Vascular endothelial growth factor-A (VEGF-A), a key regulator of angiogenesis, has several isoforms; however, their expression patterns and mechanisms of action are not fully understood. In this study, we aimed to investigate the …

jp (code pays fourni par la source)

AMPK/p38 MAPK signaling selectively enhances HIF-induced VEGF-A165 expression under hypoxic and low-glucose conditions in HepG2 cells to promote endothelial cell proliferation and migration

Hirohito Hashinokuchi, Munekazu Yamakuchi, Sadayuki Higashi, Kazunori Takenouchi et autres

Abstract Background Angiogenesis is essential for tumor development and growth. Vascular endothelial growth factor-A (VEGF-A), a key regulator of angiogenesis, has several isoforms; however, their expression patterns and mechanisms of action are not fully understood. In this study, we aimed to investigate …

jp (code pays fourni par la source)

AMPK/p38 MAPK signaling selectively enhances HIF-induced VEGF-A165 expression under hypoxic and low-glucose conditions in HepG2 cells to promote endothelial cell proliferation and migration

Hirohito Hashinokuchi, Munekazu Yamakuchi, Sadayuki Higashi, Kazunori Takenouchi et autres

Abstract Background Angiogenesis is essential for tumor development and growth. Vascular endothelial growth factor-A (VEGF-A), a key regulator of angiogenesis, has several isoforms; however, their expression patterns and mechanisms of action are not fully understood. In this study, we aimed to investigate …

jp (code pays fourni par la source)

Additional file 1 of AMPK/p38 MAPK signaling selectively enhances HIF-induced VEGF-A165 expression under hypoxic and low-glucose conditions in HepG2 cells to promote endothelial cell proliferation and migration

Hirohito Hashinokuchi, Munekazu Yamakuchi, Sadayuki Higashi, Kazunori Takenouchi et autres

Additional file 1. Protein expression levels of VEGF-A165 in conditioned medium in HepG2 cells transfected with silencing RNA targeting HIF-1α and HIF-2α (SiHIF) or control (SiC) at each glucose concentration (4.5 g/L or 0.1 g/L) under hypoxic conditions (<1.0% O2) for 24 …

jp (code pays fourni par la source)

Additional file 1 of AMPK/p38 MAPK signaling selectively enhances HIF-induced VEGF-A165 expression under hypoxic and low-glucose conditions in HepG2 cells to promote endothelial cell proliferation and migration

Hirohito Hashinokuchi, Munekazu Yamakuchi, Sadayuki Higashi, Kazunori Takenouchi et autres

Additional file 1. Protein expression levels of VEGF-A165 in conditioned medium in HepG2 cells transfected with silencing RNA targeting HIF-1α and HIF-2α (SiHIF) or control (SiC) at each glucose concentration (4.5 g/L or 0.1 g/L) under hypoxic conditions (<1.0% O2) for 24 …

jp (code pays fourni par la source)

Adaptation to acidic conditions that mimic the tumor microenvironment, downregulates miR-193b-3p, and induces EMT via TGFβ2 in A549 cells

Sadayuki Higashi, Munekazu Yamakuchi, Hirohito Hashinokuchi, Kazunori Takenouchi et autres

The acidic tumor microenvironment plays a critical role in the malignant transformation of cancer cells. One mechanism underlying this transformation involves epithelial-mesenchymal transition (EMT). This is induced by prolonged exposure to acidic conditions. EMT is an essential process in cancer progression, with …

jp (code pays fourni par la source)

Plasma and serum concentrations of VEGF-A121, but not of VEGF-A165, increase post-bevacizumab administration

Masashi Okawa, Munekazu Yamakuchi, Bibek Aryal, Kazunori Takenouchi et autres

BACKGROUND: VEGF-A concentrations were measured in the blood of bevacizumab-treated cancer patients in previous studies, but a consensus has not formed that would develop VEGF-A into a clinical biomarker. Recently, methods to strictly distinguish between the VEGF-A isoforms have been developed but …

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The Vascular Endothelial Growth Factor-A121/Vascular Endothelial Growth Factor-A165 Ratio as a Predictor of the Therapeutic Response to Immune Checkpoint Inhibitors in Gastric Cancer

Yuki Hamada, Kiyonori Tanoue, Takaaki Arigami, Munekazu Yamakuchi et autres

Background/Objectives: The response rate to immune checkpoint inhibitor (ICI) therapy is limited. Further, there is a need to discover biomarkers to predict therapeutic efficacy. The vascular endothelial growth factor (VEGF) is strongly associated with intra-tumoral immunity; however, its utility as a marker …

jp, us (code pays fourni par la source)

VEGF-A165 is the predominant VEGF-A isoform in platelets, while VEGF-A121 is abundant in serum and plasma from healthy individuals

Munekazu Yamakuchi, Masashi Okawa, Kazunori Takenouchi, Bibek Aryal et autres

Vascular endothelial growth factor A (VEGF-A) plays pivotal roles in regulating tumor angiogenesis as well as physiological vascular function. The major VEGF-A isoforms, VEGF-A121 and VEGF-A165, in serum, plasma, and platelets have not been exactly evaluated due to the lack of the …

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Evaluation of VEGF-A in platelet and microRNA-126 in serum after coronary artery bypass grafting

Kosuke Mukaihara, Munekazu Yamakuchi, Hideaki Kanda, Yoshiya Shigehisa et autres

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Dynamic changes in platelets caused by shear stress in aortic valve stenosis

Hideaki Kanda, Munekazu Yamakuchi, Kazuhisa Matsumoto, Kosuke Mukaihara et autres

BACKGROUND AND OBJECTIVE: Turbulent blood flow in patients with aortic valve stenosis (AS) results in morphological and functional changes in platelets and coagulation factors. The aim of this study is to determine how shear stress affects platelets and coagulation factors. METHODS: We …

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Processing grapefruit juice with γ-cyclodextrin attenuates its inhibitory effect on cytochrome P450 3A activity

Keishi Yamasaki, Daisuke Iohara, Yoko Oyama, Narumi Nishizaki et autres

Abstract Objectives Grapefruit (Citrus paradisi) juice enhances the oral bioavailability of drugs that are metabolized by intestinal cytochrome P450 3A4 (CYP3A4). Patients are advised to avoid drinking grapefruit juice to prevent this drug–grapefruit juice interaction. The aim of this study was to …

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