Robert F. Dierkes

Widespread BHET-hydrolyzing enzymes reveal a community-based pathway for PET transformation in the ocean

Ruth A. Schmitz, Katharina Saß, Johanna Hüttermann, Corbin Frohreich et autres

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Role of dienelactone hydrolases in PET biodegradation by flavobacteria Maribacter dokdonensis and Arenibacter palladensis

Ly T. T. Trinh, Colin Ochmann, Ifey Alio, Pablo Pérez-García et autres

ABSTRACT Dienelactone hydrolases (DLHs, EC 3.1.1.45) are bacterial enzymes primarily involved in the breakdown of cyclic esters. So far, this enzyme class is generally not well-known for PET degradation, and its potential role in plastic depolymerization remains largely unexplored. In the current …

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Enzyme-Loaded Microcapsules as Intracellular Organelles for the Degradation of Nanoplastics by Cells

Xin Liu, Jennifer Chow, Wenbo Wang, Robert F. Dierkes et autres

Increasing spills in the environment with plastic nanoparticles causes unwanted contamination. A proof-of-concept study is presented in which mammalian cells are loaded with enzymes capable of degrading capsules, here poly(ethylene terephthalate) (PET) hydrolase. Loading into cells via endocytosis is achieved by polymeric …

Enzyme-Loaded Microcapsules as Intracellular Organelles for the Degradation of Nanoplastics by Cells

Xin Liu, Jennifer Chow, Robert F. Dierkes, Neus Feliu et autres

Increasing spills in the environment with plastic nanoparticles causes unwanted contamination. A proof-of-concept study is presented in which mammalian cells are loaded with enzymes capable of degrading capsules, here poly(ethylene terephthalate) (PET) hydrolase. Loading into cells via endocytosis is achieved by polymeric …

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Microbial plastic degradation: enzymes, pathways, challenges, and perspectives

Pablo Pérez-García, Katharina Saß, Sasipa Wongwattanarat, Johannes Amann et autres

SUMMARYSynthetic polymers have transformed modern life, giving rise to a wide spectrum of versatile materials commonly known as plastics. They are essential to industries including packaging, medical devices, automotive, textiles, and many consumer goods. However, significant environmental challenges have emerged because of …

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Role of Dienelactone Hydrolases in PET Biodegradation by Flavobacteria Maribacter dokdonensis and Arenibacter palladensis

L. Trinh, Ifey Alio, Pablo Pérez-García, Sabine Keuter et autres

ABSTRACT Dienelactone hydrolases (DLHs, EC 3.1.1.45) are enzymes that play a crucial role in the breakdown of cyclic esters and some have been found to act on substrates such as terephthalate esters, which are monomers of polyethylene terephthalate (PET). In the current …

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An archaeal lid-containing feruloyl esterase degrades polyethylene terephthalate

Pablo Pérez-García, Jennifer Chow, Elisa Costanzi, Marno Gurschke et autres

Polyethylene terephthalate (PET) is a commodity polymer known to globally contaminate marine and terrestrial environments. Today, around 80 bacterial and fungal PET-active enzymes (PETases) are known, originating from four bacterial and two fungal phyla. In contrast, no archaeal enzyme had been identified …

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The metagenome‐derived esterase PET40 is highly promiscuous and hydrolyses polyethylene terephthalate ( PET )

Hongli Zhang, Robert F. Dierkes, Pablo Pérez-García, Elisa Costanzi et autres

Polyethylene terephthalate (PET) is a widely used synthetic polymer and known to contaminate marine and terrestrial ecosystems. Only few PET-active microorganisms and enzymes (PETases) are currently known, and it is debated whether degradation activity for PET originates from promiscuous enzymes with broad …

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An archaeal lid-containing feruloyl-esterase degrades polyethylene terephthalate (PET)

Wolfgang R. Streit, Pablo Pérez-García, Jennifer Chow, Elisa Costanzi et autres

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Author response for "The metagenome‐derived esterase PET40 is highly promiscuous and hydrolyses polyethylene terephthalate ( PET )"

Hongli Zhang, Robert F. Dierkes, Pablo Pérez-García, Elisa Costanzi et autres

Author response for "The metagenome‐derived esterase PET40 is highly promiscuous and hydrolyses polyethylene terephthalate ( PET )"

Hongli Zhang, Robert F. Dierkes, Pablo Pérez-García, Elisa Costanzi et autres

An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation

Robert F. Dierkes, Alan Wypych, Pablo Pérez-García, Dominik Danso et autres

Plastics and microplastics accumulate in all ecological niches. The construction of more sensitive biosensors allows to monitor and screen potential PET degradation in natural environments and industrial samples. These strains will also be a valuable tool for functional screenings of novel PETase …

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