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Emily LaMarre, Makayla R. Braunlin, Brian Kloss, Faiza Rafi et autres
ABSTRACT Braun’s lipoprotein (Lpp) is the most abundant protein present in Escherichia coli . Lpp is an outer membrane (OM) lipoprotein that contributes to the mechanical integrity of the cell envelope by tethering the OM to the underlying peptidoglycan (PGN). It has …
us, fr (code pays fourni par la source)
Henri Voedts, Phuong Chi Nguyen, Van Son Nguyen, Pauline Leverrier et autres
Proteases must be tightly regulated to prevent uncontrolled degradation, yet the mechanisms ensuring such control remain poorly understood. Members of the widespread M48 metalloprotease family are kept inactive by an autoinhibitory plug that blocks catalytic water activation, but how this plug is …
be, fr (code pays fourni par la source)
Imene Mehidi, Alima Sait-Gharout, Abdelazize Franck Bougaham, Hocine Gougam et autres
ABSTRACT Carbapenemase-producing Enterobacterales (CPEs) have globally emerged and spread beyond human compartments. However, data in wild animals, especially from low- and middle-income countries, such as Algeria, are still very scarce. Here, we investigated CPEs recovered from feces samples collected between October 2021 …
fr, Algérie (code pays fourni par la source)
Saoussen Oueslati, Nadia Jaidane, Réva Nermont, Delphine Girlich et autres
ABSTRACT Putative novel β-lactamase-encoding genes are increasingly identified in whole-genome sequencing (WGS) data, often without phenotypic or biochemical characterization. Here, we characterized the chromosome-encoded GUA-1 Ambler class A β-lactamase from the clinical isolate Pseudomonas guariconensis-like 65411. Among the 40 P. guariconensis genomes …
fr, Tunisie, ps (code pays fourni par la source)
Réva Nermont, Saoussen Oueslati, Magali Aumont‐Nicaise, Pascal Retailleau et autres
ABSTRACT OXA-48-like carbapenemases have rapidly disseminated worldwide, becoming the most common carbapenemase in many countries, with more than 60 variants reported. Among them, OXA-244 (OXA-48-R214G) and OXA-484 (OXA-181-R214G) are increasingly reported, despite overall reduced hydrolytic activities for β-lactams, including temocillin and carbapenems. …
fr (code pays fourni par la source)
Souad Fayad, Dina Daaboul, Delphine Girlich, Monzer Hamze et autres
ABSTRACT Background The Enterobacter cloacae complex (ECC) includes opportunistic pathogens that can be carbapenem resistant, thus complicating treatment regimens. Here, we characterized a carbapenem- and colistin-resistant ECC O89H7 isolate expressing KPC carbapenemase. Case Summary ECC O89H7 was isolated in January 2021 from …
fr, lb, us (code pays fourni par la source)
Nadia Jaidane, Wejdène Mansour, Saoussen Oueslati, Imene Mehidi et autres
Abstract Objectives Carbapenemase-producing Klebsiella pneumoniae (CPKp) represents a major public health threat due to limited treatment options, especially in low- and middle-income countries where colistin often remains the last active antibiotic. Here we investigated clinical carbapenem and colistin-resistant Kp isolates (CCoRKp) from …
fr, Tunisie (code pays fourni par la source)
Elisa S Pierre Despas, Seung-Hyun Cho, Bogdan Iuliu Iorga, Jean‐François Collet
The bacterial envelope is an essential compartment for survival, acting as a protective and permeability barrier. In diderm bacteria, it comprises an inner membrane, a thin peptidoglycan layer, and an outer membrane. A stable outer membrane-peptidoglycan connection is crucial for envelope integrity, …
be, fr (code pays fourni par la source)
Elisa S Pierre Despas, Seung-Hyun Cho, Bogdan Iuliu Iorga, Jean‐François Collet
The bacterial envelope is an essential compartment for survival, acting as a protective and permeability barrier. In diderm bacteria, it comprises an inner membrane, a thin peptidoglycan layer, and an outer membrane. A stable outer membrane-peptidoglycan connection is crucial for envelope integrity, …
be, fr (code pays fourni par la source)
Biniyam Kahsay Mezgebo, Ryan Chaffee, L. Ricardo Castellanos, S. Ashraf et autres
Loop-mediated isothermal amplification (LAMP) is a widely used rapid and affordable molecular DNA amplification method with minimal resource requirements. However, visual interpretation of results is subjective and prone to errors, leading to potential false-positive and negative results. To address this limitation, a …
kn, ca, fr (code pays fourni par la source)
Isabel Ramón Martínez, Hristo Nedev, Pascal Retailleau, Cécile Apel et autres
In nature, cascade reactions are used to produce specialised metabolites with highly complex architectures in just a few steps. Understanding their mechanisms paves the way for synthesising a wide variety of innovative three-dimensional structures. In this study, we present the targeted isolation …
fr, my (code pays fourni par la source)
Elisabeth Derollez, Natalia Roson-Calero, P. Rouze, Annick Dedieu-Berne et autres
Targeted-Antibacterial-Plasmids (TAPs) offer a precise approach to combat multidrug-resistant bacteria by selectively removing resistant strains while preserving commensals. Here, we assess TAPs that deliver CRISPR/Cas systems via conjugation to kill or resensitize extended-spectrum β-lactamase (ESBL)-producing Escherichia coli carrying the blaCTX-M-15 gene. We …
fr, us, es (code pays fourni par la source)
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