Sara Pistolesi

Mapping Nanoscale Protein‐Corona Kinetics of DoE‐Optimized Perfluorocarbon Encapsulated‐PLGA Nanoparticles by In Situ, Time‐Resolved Synchrotron SAXS

Joice Maria Joseph, Maria Rosa Gigliobianco, Cristina Minnelli, Paolo Moretti et autres

ABSTRACT Integrated frameworks that couple statistically guided formulation with nanoscale, time‐resolved mapping of the protein corona (PC) remain rare for perfluoro‐15‐crown‐5‐ether‐loaded poly(lactic ‐co‐ glycolic acid) nanoparticles (PFCE‐PLGA NPs). We apply a two‐phase workflow: a single‐factor screen of PLGA/surfactant molecular‐weight ( Mw ) …

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G-protein binding features and regulation of the RalGDS family member, RGL2

David Magrini, Thomas Fischer, Gilbert White, Lorenza Trabalzini et autres

RGL2 [RalGDS (Ral guanine nucleotide dissociation stimulator)-like 2] is a member of the RalGDS family that we have previously isolated and characterized as a potential effector for Ras and the Ras analogue Rap1b. The protein shares 89% sequence identity with its mouse …

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NMR Structure of Calmodulin Complexed to an N-Terminally Acetylated α-Synuclein Peptide

James M. Gruschus, Thai Leong Yap, Sara Pistolesi, Alexander S. Maltsev et autres

Calmodulin (CaM) is a calcium binding protein that plays numerous roles in Ca-dependent cellular processes, including uptake and release of neurotransmitters in neurons. α-Synuclein (α-syn), one of the most abundant proteins in central nervous system neurons, helps maintain presynaptic vesicles containing neurotransmitters …

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NMR structure of the complex of an N-terminally acetylated alpha-synuclein peptide with calmodulin

James M. Gruschus, Thai Leong Yap, Sara Pistolesi, Alexander S. Maltsev et autres

The defensin–lipid interaction: Insights on the binding states of the human antimicrobial peptide HNP-1 to model bacterial membranes

Alessio Bonucci, Enrico Balducci, Sara Pistolesi, Rebecca Pogni

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Temperature Dependence of Molecular Interactions Involved in Defining Stability of Glutamine Binding Protein and Its Complex with l -Glutamine

Sara Pistolesi, Nico Tjandra

The temperature dependence of dynamic parameters derived from nuclear magnetic resonance (NMR) relaxation data is related to conformational entropy of the system under study. This provides information such as macromolecules stability and thermodynamics of ligand binding. We studied the temperature dependence of …

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Solution NMR studies of periplasmic binding proteins and their interaction partners

Sara Pistolesi, Nico Tjandra, Guillermo A. Bermejo

Periplasmic binding proteins (PBPs) are a crucial part of ATP-binding cassette import systems in Gram-negative bacteria. Central to their function is the ability to undergo a large-scale conformational rearrangement from open-unliganded to closed-liganded, which signals the presence of substrate and starts its …

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Illuminazione naturale in ambienti confinati: monitoraggio sperimentale di un tubo di luce e validazione del modello simulato

Sara Pistolesi

SPECTROSCOPIC STUDIES ON AMPS INTERACTION WITH MODEL MEMBRANES

Alessio Bonucci, Sara Pistolesi, Enrico Balducci, Rebecca Pogni

Antimicrobial peptides (AMPs) are an essential part of innate immune defense against microbial infection. Naturally occurring AMPs are basic peptides composed of 12–50 amino acids that are ubiquitously distributed throughout all kingdoms of life. AMPs display extensive sequence heterogeneity; however they do …

Modeling the Fluorescence of Protein-Embedded Tryptophans with ab Initio Multiconfigurational Quantum Chemistry: The Limiting Cases of Parvalbumin and Monellin

Sara Pistolesi, Adalgisa Sinicropi, Rebecca Pogni, Riccardo Basosi et autres

We show that a quantum-mechanics/molecular-mechanics strategy based on ab initio (i.e., first principle) multiconfigurational perturbation theory can reproduce the spectral properties of a tryptophan residue embedded in the contrasting hydrophobic and hydrophilic environments of parvalbumin and monellin, respectively. We show that the …

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Humanin Structural Versatility and Interaction with Model Cerebral Cortex Membranes

Sara Pistolesi, Lara Rossini, Eloísa Amália Vieira Ferro, Riccardo Basosi et autres

Humanin (HN) is a recently identified neuroprotective peptide able to inhibit neurotoxicity induced by various insults which can be related to Alzheimer disease (AD) as well as to cell death induced by other stimuli. Previous CD and NMR studies demonstrated that HN …

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G-protein binding features and regulation of the RalGDS family member, RGL2

Eloísa Amália Vieira Ferro, David Magrini, Paolo Guazzi, Thomas Fischer et autres

RGL2 [RalGDS (Ral guanine nucleotide dissociation stimulator)-like 2] is a member of the RalGDS family that we have previously isolated and characterized as a potential effector for Ras and the Ras analogue Rap1b. The protein shares 89% sequence identity with its mouse …

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