Accès ouvert
2025
article
OpenAlex
Chloé Tesnière, Fadia Boudghene-Stambouli, Marc Severin, Mallorie Poët et autres
Intracellular pH (pHi) influences diverse cellular processes, including cell proliferation, metabolism, and migration, and is linked to metabolic diseases and cancer. Protonation alters protein charge and conformation, modulating different aspects of protein function. How pHi fluctuations are sensed by signaling proteins and …
ca, dk, fr
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Accès ouvert
2025
article
OpenAlex
Marc Severin, Rie Bager Hansen, Michala G. Rolver, Tove Hels et autres
Tumor acidosis alters cancer cell metabolism and favors aggressive disease progression. Cancer cells in acidic environments increase lipid droplet accumulation and oxidative phosphorylation, which are characteristics of aggressive cancers. Here, we used live imaging, shotgun lipidomics and immunofluorescence analyses of mammary and …
dk, de, il
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Michala G. Rolver, Marc Severin, Stine F. Pedersen
The expansion of cancer cell mass in solid tumors generates a harsh environment characterized by dynamically varying levels of acidosis, hypoxia, and nutrient deprivation. Because acidosis inhibits glycolytic metabolism and hypoxia inhibits oxidative phosphorylation, cancer cells that survive and grow in these …
dk
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Kathrine Lundø, Oksana Dmytriyeva, Louise Spøhr, Eliana Gonçalves‐Alves et autres
BACKGROUND: The lactate receptor GPR81 contributes to cancer development through unclear mechanisms. Here, we investigate the roles of GPR81 in three-dimensional (3D) and in vivo growth of breast cancer cells and study the molecular mechanisms involved. METHODS: GPR81 was stably knocked down …
dk
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Accès ouvert
2023
article
OpenAlex
Marc Severin, Emma Lind Pedersen, Magnus Thane Borre, Ida Axholm et autres
Finely tuned regulation of transport protein localization is vital for epithelial function. The Na+-HCO3- co-transporter NBCn1 (also known as SLC4A7) is a key contributor to epithelial pH homeostasis, yet the regulation of its subcellular localization is not understood. Here, we show that …
dk, de
(code pays fourni par la source)
Accès ouvert
2023
dataset
OpenAlex
Kathrine Lundø, Oksana Dmytriyeva, Louise Spøhr, Eliana Gonçalves‐Alves et autres
Additional file 1.
dk
(code pays fourni par la source)
Accès ouvert
2022
preprint
OpenAlex
Marc Severin, Emma Lind Pedersen, Magnus Thane Borre, Ida Axholm et autres
ABSTRACT Finely tuned regulation of transport protein localization is vital for epithelial function. Sodium-bicarbonate co-transporter NBCn1 (SLC4A7) is a key contributor to epithelial pH homeostasis, yet the regulation of its subcellular localization is not understood. Here, we show that a predicted N-terminal …
dk, de
(code pays fourni par la source)
Accès ouvert
2021
article
OpenAlex
Adélaïde Doray, Roxane Lemoine, Marc Severin, Stéphanie Chadet et autres
The SCN4B gene, coding for the NaVβ4 subunit of voltage-gated sodium channels, was recently found to be expressed in normal epithelial cells and down-regulated in several cancers. However, its function in normal epithelial cells has not been characterized. In this study, we …
fr, dk
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Accès ouvert
2021
article
OpenAlex
Lise Margrethe Sjøgaard-Frich, Andreas Prestel, Emilie S Pedersen, Marc Severin et autres
Calmodulin (CaM) engages in Ca 2+ -dependent interactions with numerous proteins, including a still incompletely understood physical and functional interaction with the human Na + /H + -exchanger NHE1. Using nuclear magnetic resonance (NMR) spectroscopy, isothermal titration calorimetry, and fibroblasts stably expressing …
dk
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Accès ouvert
2020
article
OpenAlex
Dominika Czaplińska, Line Ohrt Elingaard-Larsen, Michala G. Rolver, Marc Severin et autres
As a result of elevated metabolic rates and net acid extrusion in the rapidly proliferating cancer cells, solid tumours are characterized by a highly acidic microenvironment, while cancer cell intracellular pH is normal or even alkaline. Two-dimensional (2D) cell monocultures, which have …
dk
(code pays fourni par la source)
Accès ouvert
2020
article
OpenAlex
Mette Flinck, Sofie Hagelund, Andrej Gorbatenko, Marc Severin et autres
Increased metabolic acid production and upregulation of net acid extrusion render pH homeostasis profoundly dysregulated in many cancers. Plasma membrane activity of vacuolar H+ ATPases (V-ATPases) has been implicated in acid extrusion and invasiveness of some cancers, yet often on the basis …
dk, us, de
(code pays fourni par la source)
Accès ouvert
2020
peer-review
OpenAlex
Lise Margrethe Sjøgaard-Frich, Andreas Prestel, Emilie S Pedersen, Marc Severin et autres
NHE1-CaM complexes of multiple stoichiometries regulate cellular Ca2+-dependent NHE1 activity and can contribute to NHE1 dimerization, the latter shown by the NMR structure of CaM linking two NHE1 cytosolic tails.
dk
(code pays fourni par la source)