林福安

Hybrid data-based internal temperature prediction model for motorized spindles

Peng Li, 林福安, Lixiu Zhang, Zhaoyu Shi

Abstract As a core component of CNC machine tools, the motorized spindle experiences temperature rise during high-speed operation, which causes thermal deformation and seriously impairs machining accuracy. Accurate internal temperature prediction is the key to thermal error compensation. To address the poor …

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Serum uric acid in systemic lupus erythematosus with preserved renal function: a cross-sectional and longitudinal analysis

Xiaolu Huang, Hongpu Chen, Yulin Wang, 林福安

Objective: To evaluate the association of serum uric acid (SUA) with established lupus nephritis (LN) and to explore its prospective association with incident LN in systemic lupus erythematosus (SLE) patients presenting with preserved renal function. Methods: We enrolled 97 SLE patients with …

cn (code pays fourni par la source)

Detection of 5-Aza-2'-deoxycytidine induced p16 gene demethylation in the multiple myeloma cell line U266

付海英, 沈建箴, 叶宝国, 沈松菲 et autres

目的 探讨5-氮-2'-脱氧胞嘧啶(5-Aza-CdR)诱导骨髓瘤细胞系U266p16基因DNA5'CpG岛去甲基化作用及对U266细胞增生的影响.方法 采用巢式甲基特异性PCR法(n-MSP)、DNA序列分析、RT-PCR、细胞生长曲线、流式细胞仪DNA含量分析法检测5-Aza-CdR对U266细胞p16基因去甲基化作用及其对U266细胞的生长、增生及细胞周期的影响.结果 (1)5-Aza-CdR能够逆转U266细胞p16基因异常甲基化;(2)5-Aza-CdR能激活p16基因沉默的再转录;(3)5-Aza-CdR能下调U266细胞甲基转移酶DNMT1、DNMT3A、DNMT3B的表达并呈浓度依赖性;(4)5-Aza-CdR作用的U266细胞被阻滞于G0～G1期.结论 5-Aza-CdR可能通过抑制甲基转移酶直接对p16基因去甲基化,逆转U266细胞DNA异常甲基化,并有效地激活因高甲基化所致p16基因沉默的再转录。

5-氮-2′-脱氧胞嘧啶对U266细胞系p16基因去甲基化作用研究

付海英, 沈建箴, 叶宝国, 沈松菲 et autres