Michiyuki Matsuda

The slow-acting G-protein G z defines the duration of circadian rest time

Youichi Tanaka, Yuto Fujita, Tom Macpherson, Aoi Harui et autres

ABSTRACT Duration of nightly rest is a trait that varies between individuals, influenced by a complex interplay between multiple genetic and environmental factors. The central circadian clock that orchestrates daily rhythm in behavior/sleep resides in the suprachiasmatic nucleus (SCN). Yet, how the …

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Reevaluating the senolytic activity of a GLS1 inhibitor and an anti-PD-1 antibody: toward greater reproducibility and methodological rigor

Shimpei Kawamoto, Haruki Horiguchi, Daisuke Torigoe, Masahiro Wakita et autres

Abstract The discovery of the senescence-associated secretory phenotype (SASP) has reshaped our understanding of cellular senescence, shifting its role from a solely tumor-suppressive mechanism to a potential driver of chronic inflammation and age-related diseases. Accordingly, senolytic drugs, which selectively eliminate senescent cells, …

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Intensity modulation of trichromatic split fluorescent proteins for live cell mapping

Mamoru Ishii, Tomoaki Kinjo, Yohei Kondo, Kenta Terai et autres

Current fluorescent protein-based multiplexed cell labeling techniques suffer from limited discrimination power due to stochastic color selection and large gene sizes from tandem repeats of multiple fluorescent proteins. We developed Caterpie, a rationally designed system using engineered split fluorescent proteins that enables …

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In vivo two-photon imaging reveals stochastic and synchronized extracellular signal-regulated kinase dynamics in neurons of awake mice

Ceylin Zeybek, Yohei Kondo, Michiyuki Matsuda, Hiroko Yukinaga

The neocortex is essential for higher-order brain functions such as cognition, perception, language, and motor control. Although extracellular signal-regulated kinase (ERK) activity in neurons has been well studied for its role in memory formation, its activity patterns in the awake mice brain …

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Looking back on 50 years of cell biology in Japan

Michiyuki Matsuda

Cell Structure and Function (CSF), the official journal of the Japan Society for Cell Biology (JSCB), celebrates its 50th anniversary in 2025. This essay traces the scientific evolution of CSF from its founding in 1975 to the present, drawing on bibliometric data …

Urothelial collective-gliding response acts as a toll-like receptor 4-associated defense mechanism

Ning Zhang, Takeshi Sano, Katsuhiro Ito, Shinji Ito et autres

Collective cell migration (CCM) is characterized by the coordinated movement of cell groups while maintaining cell-to-cell cohesion. Despite extensive research on CCM, the collective migration of mature epithelial cells over the extracellular matrix in response to external stimuli has not been reported. …

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A Prospective Study on the Predictive Value of the Modified Glasgow Prognostic Score in Non-Small Cell Lung Cancer Treated with Radiation Therapy

Z. Chen, Kengo Kuriyama, M. Oguri, Masami Mutou et autres

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Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell migration

Yuya Jikko, Eriko Deguchi, Kimiya Matsuda, Naoya Hino et autres

Collective cell migration is coordinated by the front-to-rear intercellular propagation of EGFR-Ras-ERK pathway activation. However, the molecular mechanisms integrating front-to-rear information into this intercellular signaling cascade, particularly the determinants of cellular front-side specification, remain elusive. We visualized the activity of EGFR, Ras, …

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Intensity Modulation of Trichromatic Split Fluorescent Proteins for Live Cell Mapping

Mamoru Ishii, Tomoaki Kinjo, Yohei Kondo, Kenta Terai et autres

Abstract Multiplexed live cell-labeling technologies are essential tools for investigating complex biological systems, yet existing approaches face limitations in achieving reliable discrimination of multiple cell populations. Here, we present color and intensity modulation with split fluorescent proteins for cell labeling (Caterpie), a …

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E-cadherin mechanotransduction activates EGFR-ERK signaling in epithelial monolayers by inducing ADAM-mediated ligand shedding

Ronja M. Houtekamer, Mirjam C. van der Net, Marjolein J. Vliem, Tomas E. J. C. Noordzij et autres

The behavior of cells is governed by signals originating from their local environment, including mechanical forces exerted on the cells. Forces are transduced by mechanosensitive proteins, which can impinge on signaling cascades that are also activated by growth factors. We investigated the …

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E-cadherin mechanotransduction activates EGFR-ERK signaling in epithelial cells by inducing ADAM-mediated ligand shedding

Ronja M. Houtekamer, Mirjam C. van der Net, Marjolein J. Vliem, Tomas E. J. C. Noordzij et autres

Abstract The behavior of cells is governed by signals originating from their local environment, including mechanical forces that cells experience. Forces are transduced by mechanosensitive proteins, which can impinge on signaling cascades that are also activated by growth factor receptors upon ligand …

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Live imaging of paracrine signaling: Advances in visualization and tracking techniques

Eriko Deguchi, Michiyuki Matsuda, Kenta Terai

Live imaging techniques have revolutionized our understanding of paracrine signaling, a crucial form of cell-to-cell communication in biological processes. This review examines recent advances in visualizing and tracking paracrine factors through four key stages: secretion from producing cells, diffusion through extracellular space, …

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