Marylinda Famiglietti

An In-Depth Comparison of Latent HIV-1 Reactivation in Multiple Cell Model Systems and Resting CD4+ T Cells from Aviremic Patients

Vicente Planelles, Nancie M. Archin, Paula C. Soto, Akul Singhania et autres

The possibility of HIV-1 eradication has been limited by the existence of latently infected cellular reservoirs. Studies to examine control of HIV latency and potential reactivation have been hindered by the small numbers of latently infected cells found in vivo. Major conceptual …

HIV-1 Myristoylated Nef Treatment of Murine Microglial Cells Activates Inducible Nitric Oxide Synthase, NO2 Production and Neurotoxic Activity

Giorgio Mangino, Marylinda Famiglietti, Caterina Capone, Caterina Veroni et autres

BACKGROUND: The potential role of the human immunodeficiency virus-1 (HIV-1) accessory protein Nef in the pathogenesis of neuroAIDS is still poorly understood. Nef is a molecular adapter that influences several cellular signal transduction events and membrane trafficking. In human macrophages, Nef expression …

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Downmodulation of CCR7 by HIV-1 Vpu Results in Impaired Migration and Chemotactic Signaling within CD4+ T Cells

Peter W. Ramirez, Marylinda Famiglietti, Bharatwaj Sowrirajan, Ana Beatriz DePaula-Silva et autres

The chemokine receptor CCR7 plays a crucial role in the homing of central memory and naive T cells to peripheral lymphoid organs. Here, we show that the HIV-1 accessory protein Vpu downregulates CCR7 on the surface of CD4(+) T cells. Vpu and …

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An HIV-Encoded Antisense Long Noncoding RNA Epigenetically Regulates Viral Transcription

Sheena Saayman, Amanda Ackley, Anne‐Marie W. Turner, Marylinda Famiglietti et autres

The abundance of long noncoding RNAs (lncRNAs) and their wide range of functional roles in human cells are fast becoming realized. Importantly, lncRNAs have been identified as epigenetic modulators and consequently play a pivotal role in the regulation of gene expression. A …

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An In-Depth Comparison of Latent HIV-1 Reactivation in Multiple Cell Model Systems and Resting CD4+ T Cells from Aviremic Patients

Celsa A. Spina, Jenny L. Anderson, Nancie M. Archin, Alberto Bosque et autres

The possibility of HIV-1 eradication has been limited by the existence of latently infected cellular reservoirs. Studies to examine control of HIV latency and potential reactivation have been hindered by the small numbers of latently infected cells found in vivo. Major conceptual …

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HIV latency and integration site placement in five cell-based models

Scott Sherrill-Mix, Mary K. Lewinski, Marylinda Famiglietti, Alberto Bosque et autres

BACKGROUND: HIV infection can be treated effectively with antiretroviral agents, but the persistence of a latent reservoir of integrated proviruses prevents eradication of HIV from infected individuals. The chromosomal environment of integrated proviruses has been proposed to influence HIV latency, but the …

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Cyclin T1 and CDK9 T-Loop Phosphorylation Are Downregulated during Establishment of HIV-1 Latency in Primary Resting Memory CD4+T Cells

Sona Budhiraja, Marylinda Famiglietti, Alberto Bosque, Vicente Planelles et autres

P-TEFb, a cellular kinase composed of Cyclin T1 and CDK9, is essential for processive HIV-1 transcription. P-TEFb activity is dependent on phosphorylation of Thr186 in the CDK9 T loop. In resting CD4(+) T cells which are nonpermissive for HIV-1 replication, the levels …

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Homeostatic Proliferation Fails to Efficiently Reactivate HIV-1 Latently Infected Central Memory CD4+ T Cells

Alberto Bosque, Marylinda Famiglietti, Andrew S. Weyrich, Claudia Goulston et autres

Homeostatic proliferation ensures the longevity of central memory T-cells by inducing cell proliferation in the absence of cellular differentiation or activation. This process is governed mainly by IL-7. Central memory T-cells can also be stimulated via engagement of the T-cell receptor, leading …

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Recombinant Nef protein induces inflammatory response and upregulation of inducible nitric oxide synthase expression in the murine microglial cell line BV-2.

Giorgio Mangino, Marylinda Famiglietti, Gianna Fiorucci, Zulema Percario et autres