Accès ouvert
2026
preprint
OpenAlex
Carlos Piedrafita, A. D. Dickson, Daniel Richter, Caroline Weber et autres
Abstract Expanding the genetic code to enable the selective and specific incorporation of non-canonical monomers (ncMs), beyond α-L amino acids with variant sidechains, is a key outstanding challenge. Here we discover orthogonal aminoacyl-tRNA synthetases that selectively and specifically acylate their cognate orthogonal …
gb, de
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Tarun Iype, Maximilian Fottner, P. Böhm, Carlos Piedrafita et autres
Abstract The site-specific encoding of non-canonical amino acids (ncAAs) provides a powerful tool for expanding the functional repertoire of proteins 1–4 . Its widespread use for basic research and biotechnological applications is, however, hampered by the low efficiencies of current ncAA incorporation …
ch, de
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Tarun Iype, Maximilian Fottner, Carlos Piedrafita, Kathrin Lang
ABSTRACT The site-specific encoding of non-canonical amino acids (ncAAs) provides a powerful tool for expanding the functional repertoire of proteins. Its widespread use for basic research and biotechnological applications is, however, hampered by low efficiencies of current ncAA incorporation strategies. We uncover …
ch, de
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Daniel L. Dunkelmann, Carlos Piedrafita, A. D. Dickson, Kim C. Liu et autres
Abstract The genetic code of living cells has been reprogrammed to enable the site-specific incorporation of hundreds of non-canonical amino acids into proteins, and the encoded synthesis of non-canonical polymers and macrocyclic peptides and depsipeptides1–3. Current methods for engineering orthogonal aminoacyl-tRNA synthetases …
gb
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Gefei Chen, Axel Leppert, Helen Poska, Harriet E. Nilsson et autres
ATP-independent molecular chaperones are important for maintaining cellular fitness but the molecular determinants for preventing aggregation of partly unfolded protein substrates remain unclear, particularly regarding assembly state and basis for substrate recognition. The BRICHOS domain can perform small heat shock (sHSP)-like chaperone …
se, ee
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Martin Spinck, Carlos Piedrafita, Wesley E. Robertson, Thomas Elliott et autres
The direct genetically encoded cell-based synthesis of non-natural peptide and depsipeptide macrocycles is an outstanding challenge. Here we programme the encoded synthesis of 25 diverse non-natural macrocyclic peptides, each containing two non-canonical amino acids, in Syn61Δ3-derived cells; these cells contain a synthetic …
gb
(code pays fourni par la source)