Yuanhua Yu

pHEAA/pMA-DNA Hydrogel Sensing Platform for Detecting Drug-Resistant Gene Variants in Mycobacterium tuberculosis

Tingting Sun, Shuhang Li, Brij Mohan, Yuanhua Yu et autres

High Resolution Image Download MS PowerPoint Slide Mutations in the drug-resistant gene of Mycobacterium tuberculosis can make it challenging to use drugs in clinical practice. Traditional genetic testing for resistance requires cell culture and susceptibility testing, which take 1–2 weeks. In this …

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Research on the Combination of Isothermal Amplification and Immunochromatography Detection Method of Novel Coronavirus Nucleic Acid

Tengfei Liu, Ye Ji, Hongying Wang, Yuanhua Yu

Objective: To develop a rapid and sensitive detection method for SARS-CoV-2 by combining recombinase polymerase amplification (RPA) with fluorescence immunochromatography. Methods: Specific primers targeting the SARS-CoV-2 nucleocapsid (N) protein gene were designed and modified with fluorescein isothiocyanate (FITC) and biotin. RPA conditions, …

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Analytical Device and Prediction Method for Urine Component Concentrations

Zhe Wang, Jian Huang, Qimeng Chen, Yuanhua Yu et autres

To tackle the low-accuracy problem with analyzing urine component concentrations in real time, a fully automated dipstick analysis device of urine dry chemistry was designed, and a prediction method combining an image acquisition system with a whale optimization algorithm (WOA) for BP …

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Research on a device for fluorescence imaging detection of nucleic acid

Luyang Duanmu, Yuanhua Yu, Hongzhuang Guo

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Research on a Device for Fluorescence Imaging Detection of Nucleic

Luyang Duanmu, Yuanhua Yu

With the rapid development of nucleic acid molecule detection technology, nucleic acid detection devices have become a global research hotspot in recent years. This paper uses multidisciplinary fusion technology to propose a nucleic acid fluorescence detection design scheme. The device is mainly …

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A Cell Microscopy Image Recognition Method Based on Convolutional Neural Networks

Yuanhua Yu

This paper proposes a deep learning cell recognition algorithm based on an improved Inception-V3 model. The algorithm first preprocesses the images and creates a dataset. Then, it modifies the Inception-V3 model by adding and improving layers to retain more local detail information …

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Establishment of a Detection Methodology for CAR-T Cell Vector Copy Number Using Droplet Digital PCR

Xiao Xiao, Le Gao, Yuanhua Yu, Xinyu Chen

Objective: To explore the establishment of a detection method for the vectors copy number of Chimeric Antigen Receptor T cells (CAR-T) in patients undergoing CAR-T immunotherapy, based on droplet digital PCR (ddPCR) technology, and to evaluate the performance of this method. Methods: …

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Real-Time Fluorescent Quantitative PCR for Detection of Peripheral Blood T-Cell Lymphoma

Ren Xu, Han Zhang, Yuanhua Yu, Yibing Jia et autres

Objective: Peripheral T-cell lymphoma (PTCL) is a group of rare and highly heterogeneous malignant diseases of the lymphatic system derived from mature, post-thymic T cells. In this work, a qPCR assay was constructed for RHOA G17V and IDH2 R172 mutations by extracting …

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Development of Dual Fluorescence qPCR for the Detection of Pseudomonas Aeruginosa

Han Zhang, Hao Zhang, Yuanhua Yu

Objective: To establish a real-time fluorescence quantitative PCR method for the detection of Pseudomonas aeruginosa (PA), which provides an effective method for the rapid and accurate clinical detection of PA. Methods: Using the principle of fluorescence quantitative PCR technology, the relatively conserved …

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Study on the Detection Method of Rheumatoid Factor Based on Nano-Microspheres

Yuanhua Yu, Xiao Xiao

Rheumatoid factor (RF) plays an important role in clinical practice as an important indicator for detecting immunologic diseases. The aim of this study was to develop a system for the detection of rheumatoid factor (RF) using nanomicrosphere immunoturbidimetric assay (PETIA). This study …

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Screening and Application ofHEp-2 Cell Culture Medium without Ingredients of Animal Origin

Jin‐Hui Yang, Yuanhua Yu, Anqi Sheng

Indirect immunofluorescence is a commonly used method for preliminary diagnosis of autoimmune diseases, which has the characteristics of simplicity and high specificity. Human laryngeal cancer cells (HEp-2 cells) are the main raw material for preparing indirect immunofluorescence detection kits. Driven by the …

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Characterization of a Meso-Butanediol Dehydrogenase in Comamonas testosteroni ATCC11996

Xiangyu Shi, Hao Zhang, Yuanhua Yu, Sichen Zhang

Comamonas testosteroni is a steroid hormones degradation bacterium and it is also an opportunistic pathogen can cause bacteremia in human. In this work, a MBD gene was knocked out in C. testosteroni ATCC11996 which was up regulated induced with testosterone in transcriptome, …

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