Murat Artan

Proximity labeling of DAF-16 FOXO highlights aging regulatory proteins

Murat Artan, Hanna Schoen, Mario de Bono

Insulin/insulin-like growth factor signaling inhibits FOXO transcription factors to control development, homeostasis, and aging. Here, we use proximity labeling to identify proteins interacting with the C. elegans FOXO DAF-16. We show that in well-fed, unstressed animals harboring active insulin signaling, DAF-16 forms …

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Statistical analysis of lifespan data.

Sang‐Soon Park, Yoonji Jung, Seon Woo A. An, Heehwa G. Son et autres

Background: α-arrestins are a family of proteins that are implicated in multiple biological processes, including metabolism and receptor desensitization. Methods: Here, we sought to examine the roles of α-arrestins in the longevity of Caenorhabditis elegans through an RNA interference screen. Results: We …

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Mean lifespan and mortality rates of RNAi experiment results.

Sang‐Soon Park, Yoonji Jung, Seon Woo A. An, Heehwa G. Son et autres

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Kaplan-Meier estimator of RNAi lifespan experiments.

Sang‐Soon Park, Yoonji Jung, Seon Woo A. An, Heehwa G. Son et autres

Background: α-arrestins are a family of proteins that are implicated in multiple biological processes, including metabolism and receptor desensitization. Methods: Here, we sought to examine the roles of α-arrestins in the longevity of Caenorhabditis elegans through an RNA interference screen. Results: We …

kr (code pays fourni par la source)

Depletion of endogenously biotinylated carboxylases enhances the sensitivity of TurboID-mediated proximity labeling in Caenorhabditis elegans

Murat Artan, Markus Hartl, Weiqiang Chen, Mario de Bono

Proximity-dependent protein labeling provides a powerful in vivo strategy to characterize the interactomes of specific proteins. We previously optimized a proximity labeling protocol for Caenorhabditis elegans using the highly active biotin ligase TurboID. A significant constraint on the sensitivity of TurboID is …

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Correction: Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling

Murat Artan, Stephen McG Barratt, Sean M. Flynn, Farida Begum et autres

During the typesetting of the article above an error was introduced that resulted in 9 missing nucleotides on page 10. Please see the corrections, which are set in red in Figure below. Production apologizes for the error. Interactome analysis of Caenorhabditis elegans …

An improved TurboID pipeline in C. elegans by biochemical depletion of endogenously biotinylated carboxylases

Murat Artan, Markus Hartl, Weiqiang Chen, Mario de Bono

Abstract Proximity-dependent protein labeling provides a powerful in vivo strategy to characterize the interactomes of specific proteins. We previously optimized a proximity labeling protocol for C. elegans using the highly active biotin ligase TurboID. A significant constraint on the sensitivity of TurboID …

at (code pays fourni par la source)

MON-2, a Golgi protein, promotes longevity by upregulating autophagy through mediating inter-organelle communications

Murat Artan, Jooyeon Sohn, Cheolju Lee, Seung‐Yeol Park et autres

The Golgi apparatus regulates the process of modification and subcellular localization of macromolecules, including proteins and lipids. Aberrant protein sorting caused by defects in the Golgi leads to various diseases in mammals. However, the role of the Golgi apparatus in organismal longevity …

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Proteomic Analysis of C. Elegans Neurons Using TurboID-Based Proximity Labeling

Murat Artan, Mario de Bono

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MON-2, a Golgi protein, mediates autophagy-dependent longevity in Caenorhabditis elegans

Yoonji Jung, Murat Artan, Nari Kim, Jeonghun Yeom et autres

. Consistently, we showed that mammalian MON2 activated GABARAPL2 through physical interaction, which increased autophagic flux in mammalian cells. Thus, the evolutionarily conserved role of MON2 in trafficking between the Golgi and endosome is an integral part of autophagy-mediated longevity.

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Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling

Murat Artan, Stephen Barratt, Sean M. Flynn, Farida Begum et autres

Proximity labeling provides a powerful in vivo tool to characterize the proteome of subcellular structures and the interactome of specific proteins. The nematode Caenorhabditis elegans is one of the most intensely studied organisms in biology, offering many advantages for biochemistry. Using the …

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Interactome analysis of C. elegans synapses by TurboID-based proximity labeling

Murat Artan, Stephen Barratt, Sean M. Flynn, Farida Begum et autres

Abstract Proximity labelling provides a powerful in vivo tool to characterize the proteome of sub-cellular structures and the interactome of specific proteins. Using the highly active biotin ligase TurboID, we optimize a proximity labelling protocol for C. elegans . We use this …

at, gb (code pays fourni par la source)