Natalie D. Di Bartolo

Hybrid B- and T-Cell Immunity Associates With Protection Against Breakthrough Infection After Severe Acute Respiratory Syndrome Coronavirus 2 Vaccination in Avon Longitudinal Study of Parents and Children (ALSPAC) Participants

Holly E. Baum, Marianna Santopaolo, Ore Francis, Emily Milodowski et autres

BACKGROUND: Immunological memory to vaccination and viral infection involves the coordinated action of B and T cells; thus, integrated analysis of these 2 components is critical for understanding their respective contributions to protection against breakthrough infections (BIs) after vaccination. METHODS: We investigated …

gb, tw (code pays fourni par la source)

Hybrid adaptive immunity to SARS-CoV-2 protects against breakthrough infection after COVID-19 vaccination in ALSPAC participants

Holly E. Baum, Marianna Santopaolo, Ore Francis, Emily Milowdowski et autres

SUMMARY Immunological memory to vaccination and viral infection involves coordinated action of B and T-cells, thus integrated analysis of these two components is critical for understanding their contributions to protection against breakthrough infections (BI). We investigated cellular and humoral immune responses to …

gb (code pays fourni par la source)

Gut microorganism enzymes unlock universal blood

Timothy J. Satchwell, Natalie D. Di Bartolo, Ashley Mark Toye

gb (code pays fourni par la source)

Evaluation and deployment of isotype-specific salivary antibody assays for detecting previous SARS-CoV-2 infection in children and adults

Amy C Thomas, Elizabeth Oliver, Holly E. Baum, Kapil Gupta et autres

BACKGROUND: Saliva is easily obtainable non-invasively and potentially suitable for detecting both current and previous SARS-CoV-2 infection, but there is limited evidence on the utility of salivary antibody testing for community surveillance. METHODS: We established 6 ELISAs detecting IgA and IgG antibodies …

gb, pt (code pays fourni par la source)

Development and evaluation of low-volume tests to detect and characterize antibodies to SARS-CoV-2

Alice Halliday, Anna E. Long, Holly E. Baum, Amy C Thomas et autres

Low-volume antibody assays can be used to track SARS-CoV-2 infection rates in settings where active testing for virus is limited and remote sampling is optimal. We developed 12 ELISAs detecting total or antibody isotypes to SARS-CoV-2 nucleocapsid, spike protein or its receptor …

gb, it (code pays fourni par la source)

Development and evaluation of low-volume tests to detect and characterise antibodies to SARS-CoV-2

Alice Halliday, Anna E. Long, Holly E. Baum, Amy C Thomas et autres

Abstract Low-volume antibody assays can be used to track SARS-CoV-2 infection rates in settings where active testing for virus is limited and remote sampling is optimal. We developed 12 ELISAs detecting total or antibody isotypes to SARS-CoV-2 nucleocapsid, spike protein or its …

gb, it (code pays fourni par la source)

Evaluation of isotype specific salivary antibody assays for detecting previous SARS-CoV-2 infection in children and adults

Amy C Thomas, Elizabeth Oliver, Holly E. Baum, Kapil Gupta et autres

Abstract Saliva is easily obtainable non-invasively and potentially suitable for detecting both current and previous SARS-CoV-2 infection. We established 6 standardised enzyme linked immunosorbent assays (ELISA) capable of detecting IgA and IgG antibodies to whole SARS-CoV-2 spike protein, to its receptor binding …

gb, pt (code pays fourni par la source)

Young infants exhibit robust functional antibody responses and restrained IFN-γ production to SARS-CoV-2

Anu Goenka, Alice Halliday, Michaela Gregorová, Emily Milodowski et autres

Severe COVID-19 appears rare in children. This is unexpected, especially in young infants, who are vulnerable to severe disease caused by other respiratory viruses. We evaluate convalescent immune responses in 4 infants under 3 months old with confirmed COVID-19 who presented with …

gb, it (code pays fourni par la source)

Mechanisms of Self‐Assembly and Energy Harvesting in Tuneable Conjugates of Quantum Dots and Engineered Photovoltaic Proteins

Juntai Liu, Judith Mantell, Natalie D. Di Bartolo, Michael R. Jones

Photoreaction centers facilitate the solar energy transduction at the heart of photosynthesis and there is increasing interest in their incorporation into biohybrid devices for solar energy conversion, sensing, and other applications. In this work, the self-assembly of conjugates between engineered bacterial reaction …

gb (code pays fourni par la source)

Directed assembly of defined oligomeric photosynthetic reaction centres through adaptation with programmable extra-membrane coiled-coil interfaces

David J. K. Swainsbury, Robert L. Harniman, Natalie D. Di Bartolo, Juntai Liu et autres

A challenge associated with the utilisation of bioenergetic proteins in new, synthetic energy transducing systems is achieving efficient and predictable self-assembly of individual components, both natural and man-made, into a functioning macromolecular system. Despite progress with water-soluble proteins, the challenge of programming …

gb (code pays fourni par la source)

Complete Reversible Refolding of a G-Protein Coupled Receptor on a Solid Support

Natalie D. Di Bartolo, Emma L. R. Compton, Tony Warne, Patricia C. Edwards et autres

The factors defining the correct folding and stability of integral membrane proteins are poorly understood. Folding of only a few select membrane proteins has been scrutinised, leaving considerable deficiencies in knowledge for large protein families, such as G protein coupled receptors (GPCRs). …

gb, ch (code pays fourni par la source)

Stable folding core in the folding transition state of an α-helical integral membrane protein

Paul Curnow, Natalie D. Di Bartolo, Kathleen M. Moreton, Oluseye O. Ajoje et autres

Defining the structural features of a transition state is important in understanding a folding reaction. Here, we use Φ-value and double mutant analyses to probe the folding transition state of the membrane protein bacteriorhodopsin. We focus on the final C-terminal helix, helix …

gb (code pays fourni par la source)