Dylan Hong Zheng Koh

Cholesterol maintains the degradative capacity of lysosomes during clearance and recycling of dysfunctional mitochondria

Haoning Yang, Koji Matsuhisa, Teng Wei, Wataru Nishi et autres

Efficient clearance and recycling of dysfunctional mitochondria through the robust catabolic activity of lysosomes are essential for cellular health. However, how membrane lipids contribute to maintaining the degradative capacity of lysosomes remains poorly understood. Here, we show that cholesterol plays a critical …

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Intracellular lipid transport : methods and protocols

Guillaume Drin, Tomoki Naito, Minyoung Na, Dylan Hong Zheng Koh et autres

N.A.

Using GRAM Domain–Based Biosensors to Monitor Accessible Cholesterol Distribution in Live Cells

Tomoki Naito, Minyoung Na, Dylan Hong Zheng Koh, Yasunori Saheki

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Abstract 1338 Visualization of accessible cholesterol in cellular membranes using a GRAM domain-based biosensor

Dylan Hong Zheng Koh, Tomoki Naito, Minyoung Na, Yee Jie Yeap et autres

Cholesterol plays a significant role in maintaining membrane integrity and cell signaling. Dysregulation of the distribution of cellular cholesterol is associated with numerous diseases, including cardiovascular and neurological diseases. While regulated transport of a specific pool of cholesterol, known as “accessible cholesterol” …

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Elucidating the mechanisms of intracellular cholesterol homeostasis using a novel cholesterol biosensor

Dylan Hong Zheng Koh

Cholesterol plays a significant role in maintaining membrane integrity and cell signaling. Dysregulation of the distribution of cellular cholesterol is associated with numerous diseases, including cardiovascular and neurological diseases. While regulated transport of a specific pool of cholesterol, known as “accessible cholesterol” …

Visualization of accessible cholesterol using a GRAM domain-based biosensor

Dylan Hong Zheng Koh, Tomoki Naito, Minyoung Na, Yee Jie Yeap et autres

Cholesterol is important for membrane integrity and cell signaling, and dysregulation of the distribution of cellular cholesterol is associated with numerous diseases, including neurodegenerative disorders. While regulated transport of a specific pool of cholesterol, known as "accessible cholesterol", contributes to the maintenance …

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Regulation of cellular cholesterol distribution via non-vesicular lipid transport at ER-Golgi contact sites

Tomoki Naito, Haoning Yang, Dylan Hong Zheng Koh, Divyanshu Mahajan et autres

Abnormal distribution of cellular cholesterol is associated with numerous diseases, including cardiovascular and neurodegenerative diseases. Regulated transport of cholesterol is critical for maintaining its proper distribution in the cell, yet the underlying mechanisms remain unclear. Here, we show that lipid transfer proteins, …

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Molecular basis of accessible plasma membrane cholesterol recognition by the GRAM domain of GRAMD1b

Bilge Ercan, Tomoki Naito, Dylan Hong Zheng Koh, Dennis Dharmawan et autres

Cholesterol is essential for cell physiology. Transport of the "accessible" pool of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) by ER-localized GRAMD1 proteins (GRAMD1a/1b/1c) contributes to cholesterol homeostasis. However, how cells detect accessible cholesterol within the PM remains …

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Regulation of Plasma Membrane Sterol Homeostasis by Nonvesicular Lipid Transport

Dylan Hong Zheng Koh, Yasunori Saheki

Sterol contributes to the structural integrity of cellular membranes and plays an important role in the regulation of cell signaling in eukaryotes. It is either produced in the endoplasmic reticulum or taken up from the extracellular environment. In most eukaryotic cells, however, …

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Phosphatase POPX2 interferes with cell cycle by interacting with Chk1

Pu Rum Kim, Yen Ling Koon, Raphael Tze Chuen Lee, Farouq Azizan et autres

Protein-protein interaction network analysis plays critical roles in predicting the functions of target proteins. In this study, we used a combination of SILAC-MS proteomics and bioinformatic approaches to identify Checkpoint Kinase 1 (Chk1) as a possible POPX2 phosphatase interacting protein. POPX2 is …

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Movement of accessible plasma membrane cholesterol by the GRAMD1 lipid transfer protein complex

Tomoki Naito, Bilge Ercan, Logesvaran Krshnan, Alexander Triebl et autres

Cholesterol is a major structural component of the plasma membrane (PM). The majority of PM cholesterol forms complexes with other PM lipids, making it inaccessible for intracellular transport. Transition of PM cholesterol between accessible and inaccessible pools maintains cellular homeostasis, but how …

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Author response: Movement of accessible plasma membrane cholesterol by the GRAMD1 lipid transfer protein complex

Tomoki Naito, Bilge Ercan, Logesvaran Krshnan, Alexander Triebl et autres

The human body contains trillions of cells. At the outer edge of each cell is the plasma membrane, which protects the cell from the external environment. This membrane is mostly made of fatty molecules known as lipids and about half of these …

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