Jarosław Sędzicki

Structural basis for antibiotics murepavadin and thanatin targeting the lipopolysaccharide insertase LptD

Sebastian Hiller, Philippe A. Lehner, Laetitia Rožić, Seyed Majed Modaresi et autres

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Intracellular processing of DNA-lipid nanoparticles: A quantitative assessment by image segmentation

Alessandra Cavegn, Samuel Waldner, David C. Wang, Jarosław Sędzicki et autres

Carriers for efficient delivery of nucleic acids, such as lipid nanoparticles (LNPs), have gained much attention for gene therapy applications. Intracellular processing of such nanocarriers is a complex mechanism comprising cellular internalization by endocytosis pathways, endosomal release into the cytosol, lysosomal degradation, …

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Nano Plasma Membrane Vesicle‐Lipid Nanoparticle Hybrids for Enhanced Gene Delivery and Expression

Claudio Luca Alter, Claudia Lotter, Ramya Deepthi Puligilla, Jan Stephan Bolten et autres

Lipid nanoparticles (LNPs) have emerged as the leading nonviral nucleic acid (NA) delivery system, gaining widespread attention for their use in COVID-19 vaccines. They are recognized for their efficient NA encapsulation, modifiability, and scalable production. However, LNPs face efficacy and potency limitations …

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The putative type 4 secretion system effector BspD is involved in maintaining envelope integrity of the pathogen Brucella

Maren Ketterer, Petra Chiquet, Mara Esposito, Jarosław Sędzicki et autres

ABSTRACT Brucellosis is a debilitating disease caused by the Gram-negative, facultative intracellular zoonotic pathogen Brucella. En route to its intracellular replicative niche, Brucella encounters various stressful environments that may compromise envelope integrity. Here we show that the proposed type 4 secretion system …

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The putative Type 4 secretion system effector BspD is involved in maintaining envelope integrity of the pathogen Brucella

Maren Ketterer, Petra Chiquet, Jarosław Sędzicki, Maxime Québatte et autres

Abstract Brucellosis is a debilitating disease caused by the Gram-negative, facultative intracellular zoonotic pathogen Brucella . En route to its intracellular replicative niche, Brucella encounters various stressful environments that may compromise envelop integrity. Here we show, that the proposed Type 4 secretion …

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Structure-function analysis of the cyclic β-1,2-glucan synthase from Agrobacterium tumefaciens

Jarosław Sędzicki, Dongchun Ni, Frank Serge Lehmann, Henning Stahlberg et autres

The synthesis of complex sugars is a key aspect of microbial biology. Cyclic β-1,2-glucan (CβG) is a circular polysaccharide critical for host interactions of many bacteria, including major pathogens of humans (Brucella) and plants (Agrobacterium). CβG is produced by the cyclic glucan …

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Host cell egress of Brucella abortus requires BNIP3L‐mediated mitophagy

Jérémy Verbeke, Youri Fayt, Lisa Joy Martin, Oya Yılmaz et autres

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Structure-function analysis of the cyclic β-1,2-glucan synthase

Jarosław Sędzicki, Dongchun Ni, Frank Serge Lehmann, Henning Stahlberg et autres

Abstract The synthesis of complex sugars is a key aspect of microbial biology. Cyclic β-1,2-glucan (CβG) is a circular polysaccharide critical for host interactions of many bacteria, including major pathogens of humans ( Brucella ) and plants ( Agrobacterium ). CβG is …

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Guided-deconvolution for correlative light and electron microscopy

Fengjiao Ma, Rainer Marco Kaufmann, Jarosław Sędzicki, Zoltán Cseresnyés et autres

Correlative light and electron microscopy is a powerful tool to study the internal structure of cells. It combines the mutual benefit of correlating light (LM) and electron (EM) microscopy information. The EM images only contain contrast information. Therefore, some of the detailed …

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Mechanism of cyclic β-glucan export by ABC transporter Cgt of Brucella

Jarosław Sędzicki, Dongchun Ni, Frank Serge Lehmann, Na Wu et autres

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BNIP3L-mediated mitophagy triggered by Brucella in host cells is required for bacterial egress

Jérémy Verbeke, Youri Fayt, Lisa Joy Martin, Oya Yılmaz et autres

ABSTRACT The facultative intracellular pathogen Brucella abortus interacts with several organelles of the host cell to reach its replicative niche inside the endoplasmic reticulum. However, little is known about the interplay between the bacteria and the host cell mitochondria. Here, we showed …

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Guided-deconvolution for Correlative Light and Electron Microscopy

Fengjiao Ma, Rainer Marco Kaufmann, Jarosław Sędzicki, Zoltán Cseresnyés et autres

Correlative light and electron microscopy is a powerful tool to study the internal structure of cells. It combines the mutual benefit of correlating light (LM) and electron (EM) microscopy information. However, the classical approach of overlaying LM onto EM images to assign …