Stuart M. Brierley

A Neuroendocrine Circuit That Suppresses Excretion and Pelvic Pain During Activity

Lucas E. Cabrera Zapata, Archana Venkataraman, Andrea M. Harrington, Jitao Hu et autres

ABSTRACT Mate-seeking during peak sexual receptivity is tightly coupled with exploratory movement, a process made more efficient by transient suppression of excretion. Here, we define a functional hypothalamic-hindbrain circuit in mice that promotes locomotion while eliminating urination and defecation for hours. Chemogenetic …

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Bortezomib-induced gastrointestinal dysfunction, peripheral neuropathy and neuroimmune signalling are influenced by the gut microbiota in mice

Jacqui S. Scott, Sadia Munir, Miriam A. Lynn, Jvaughn A. Duggan et autres

BACKGROUND: Peripheral neuropathy and gastrointestinal dysfunction are frequent, debilitating side effects of the neurotoxic myeloma drug bortezomib that reduce quality of life and adherence to optimal therapy. Therapeutic strategies to manage these complications are limited. Here, we have developed a mouse model …

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Fundamentals of Neurogastroenterology: Basic Science

Beverley Greenwood–Van Meerveld, Gary M. Mawe, Arthur Beyder, Stuart M. Brierley et autres

This review highlights major advances in neurogastroenterology since Rome IV, offering a condensed summary of a comprehensive document to appear in the forthcoming Rome V book. Prepared by an international team of experts, it emphasizes pivotal studies that have deepened understanding of …

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Parasites trigger epithelial cell crosstalk to drive gut–brain signalling

Kouki K Touhara, Jinhao Xu, Joel Castro, Hong-Erh Liang et autres

Abstract Parasitic infections modulate both immune and sensory responses, but how these systems collaborate to elicit protective behaviours remains incompletely understood. The gut epithelium contains specialized sensory cells that detect pathogens and irritants. These include cholinergic tuft cells, which sense parasites and …

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Nature-derived products and peptides: emerging insights into the mechanisms of visceral pain

Mariana Brizuela, Stuart M. Brierley

Naturally derived products, including plant- and venom-derived peptides, have played an instrumental role in identifying the ion channels, receptors and signalling pathways involved in visceral pain. Although crude venoms are traditionally known for their harmful effects, research into their molecular composition has …

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Ex vivo nerve recordings in NaV1-8-Cre X CHR2 mice

Stuart M. Brierley, Joel Castro, David Julius

Ex vivo single-fiber recordings of colonic pelvic mucosal afferents using optogenetic stimulation at baseline and following isovalerate challenge.

Ex vivo nerve recordings in CHRM3 Tac1-Cre and PfTox Tac1-Cre mice

Stuart M. Brierley, Joel Castro, Mariana Brizuela

Ex vivo single-fiber recordings of colonic pelvic mucosal afferents using optogenetic stimulation at baseline and following isovalerate challenge.

Visceromotor responses (VMR) to colorectal distension in female and male mice with intracolonic isovalerate

Stuart M. Brierley, Gudrun Schober, Jessica Maddern, Joel Castro et autres

Visceromotor responses (VMR) to colorectal distension (CRD) measured by EMG recordings from electrodes implanted in the abdominal musculature.

Electrophysiological recordings of mouse colonic afferent responses to mechanical and pharmacological stimuli in ex vivo preparations

Stuart M. Brierley, Mariana Brizuela

Ex vivo colonic afferent firing was recorded in response to ramp distension and drug infusion.

Intravital calcium imaging of dorsal root ganglion sensory neurons in response to intracolonic isovalerate in mice

Stuart M. Brierley, Joel Castro

Activity of sensory neurons within intact L6 DRG ganglia were imaged in confocal microscope in anesthetized NaV1.8 GCaMP6s healthy naive mice. Time course of the effect of Isovalerate (with and without Alosetron) on sensory neurons activity was studied.

A cellular basis for heightened gut sensitivity in females

Archana Venkataraman, Eric E. Figueroa, Joel Castro, Fernanda Castro Navarro et autres

Visceral pain disorders, such as irritable bowel syndrome, exhibit a marked female prevalence. Enhanced signaling between enterochromaffin (EC) cells in the gut epithelium and mucosal sensory nerve fibers likely contributes to this sex bias. We identified an estrogen-responsive paracrine pathway in which …

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GABA B receptor‐mediated modulation of sensory neuron excitability: Roles of Ca V 2.2, G‐protein‐coupled inwardly rectifying potassium (GIRK) channels, and hyperpolarisation‐activated cyclic nucleotide‐gated (HCN) channels in human and mouse nociception

Mariana Brizuela, Anuja R. Bony, Sonia Garcia‐Caraballo, David J. Adams et autres

Abstract Chronic visceral pain is a key symptom of irritable bowel syndrome. Modulation of voltage‐gated calcium and potassium channels by G protein‐coupled receptors plays a key role in dampening nociceptive transmission. Both baclofen and the analgesic peptide α‐conotoxin Vc1.1 activate GABA B …

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