Rina Yogo

Temporal and Sex-Dependent N-Glycosylation Dynamics in Rat Serum

Hirokazu Yagi, Sachiko Kondo, Reiko Murakami, Rina Yogo et autres

We conducted systematic glycomic and glycoproteomic profiling to characterize the dynamic N-glycosylation landscape of rat serum, with particular focus on sex- and time-dependent variations. MALDI-TOF-MS analysis revealed that rat serum N-glycans are predominantly biantennary, disialylated complex-type structures with extensive O-acetylation of Neu5Ac …

jp (code pays fourni par la source)

A multi-kingdom genetic barcoding system for precise clone isolation

Soh Ishiguro, Kana Ishida, Rina C. Sakata, Minori Ichiraku et autres

Cell-tagging strategies with DNA barcodes have enabled the analysis of clone size dynamics and clone-restricted transcriptomic landscapes in heterogeneous populations. However, isolating a target clone that displays a specific phenotype from a complex population remains challenging. Here we present a multi-kingdom genetic …

ca, jp (code pays fourni par la source)

Quantitative Analysis of Therapeutic Antibody Interactions with Fcγ Receptors Using High-Speed Atomic Force Microscopy

Saeko Yanaka, Hiroki Watanabe, Rina Yogo, Mesayamas Kongsema et autres

This study employed high-speed atomic force microscopy to quantitatively analyze the interactions between therapeutic antibodies and Fcγ receptors (FcγRs). Antibodies are essential components of the immune system and are integral to biopharmaceuticals. The focus of this study was on immunoglobulin G molecules, …

jp, us, th (code pays fourni par la source)

Negative interference with antibody-dependent cellular cytotoxicity mediated by rituximab from its interactions with human serum proteins

Saeko Yanaka, Rina Yogo, Hirokazu Yagi, Masayoshi Onitsuka et autres

Although interactions of small molecular drugs with serum proteins have been widely studied from pharmacokinetic and pharmacodynamic perspectives, there have been few reports on the effects of serum components on therapeutic antibody functions. This study reports the effect of abundant serum proteins …

jp (code pays fourni par la source)

The Advantage of Small-angle Neutron Scattering Revealed through Analyzing the Overall Structure of a Fully Assembled Complex in Circadian Clock

Yasuhiro Yunoki, Atsushi Matsumoto, Ken Morishima, Anne Martel et autres

Cyanobacterial clock proteins; KaiA, KaiB and KaiC are periodically assembled into the large complex (ABC complex) in a 24-hour period in vitro. A cryo-EM study reported the structure of ABC complex. However, the locations of N-terminal domains of KaiA (AN domains) have …

jp, fr, us (code pays fourni par la source)

The Fab portion of immunoglobulin G has sites in the CL domain that interact with Fc gamma receptor IIIa

Yuki Yamaguchi, Natsumi Wakaizumi, Mine Irisa, Takahiro Maruno et autres

The interaction between IgG and Fc gamma receptor IIIa (FcγRIIIa) is essential for mediating immune responses. Recent studies have shown that the antigen binding fragment (Fab) and Fc are involved in IgG-FcγRIII interactions. Here, we conducted bio-layer interferometry (BLI) and isothermal titration …

jp (code pays fourni par la source)

Overall structure of fully assembled cyanobacterial KaiABC circadian clock complex by an integrated experimental-computational approach

Yasuhiro Yunoki, Atsushi Matsumoto, Ken Morishima, Anne Martel et autres

In the cyanobacterial circadian clock system, KaiA, KaiB and KaiC periodically assemble into a large complex. Here we determined the overall structure of their fully assembled complex by integrating experimental and computational approaches. Small-angle X-ray and inverse contrast matching small-angle neutron scatterings …

jp, fr, ca (code pays fourni par la source)

Quantitative Visualization of the Interaction between Complement Component C1 and Immunoglobulin G: The Effect of CH1 Domain Deletion

Saeko Yanaka, Shigetaka Nishiguchi, Rina Yogo, Hiroki Watanabe et autres

Immunoglobulin G (IgG) adopts a modular multidomain structure that mediates antigen recognition and effector functions, such as complement-dependent cytotoxicity. IgG molecules are self-assembled into a hexameric ring on antigen-containing membranes, recruiting the complement component C1q. In order to provide deeper insights into …

jp (code pays fourni par la source)

Glutamine-free mammalian expression of recombinant glycoproteins with uniform isotope labeling: an application for NMR analysis of pharmaceutically relevant Fc glycoforms of human immunoglobulin G1

Saeko Yanaka, Hirokazu Yagi, Rina Yogo, Masayoshi Onitsuka et autres

jp (code pays fourni par la source)

Quantitative Visualization of the Interaction between Complement Component C1 and Immunoglobulin G: The Effect of CH1 Domain Deletion

Saeko Yanaka, Shigetaka Nishiguchi, Rina Yogo, Hiroki Watanabe et autres

Immunoglobulin G (IgG) adopts a modular multidomain structure that mediates antigen recognition and effector functions, such as complement-dependent cytotoxicity. IgG molecules are self-assembled into a hexameric ring on antigen-containing membranes, recruiting the complement component, C1q. To provide deeper insights into the initial …

jp, ca (code pays fourni par la source)

Structural analysis of antibody complexes by inverse contrast-matching small-angle neutron scattering combined with size-exclusion chromatography (SEC-iCM-SANS)

Nobuhiro Sato, Rina Yogo, Saeko Yanaka, Anne Martel et autres

jp, fr (code pays fourni par la source)

Analysis of antibody interaction and function in serum

Rina Yogo