Kari D. Hagen

Breaking the annotation barrier: An initial subcellular localization atlas of Giardia ’s hypothetical and conserved hypothetical proteins provides a resource for functional discovery

Kari D. Hagen, Alexander Zerkle, Satvik Arani, Tiffany M. Chase et autres

Giardia intestinalis is a globally prevalent cause of waterborne diarrheal disease, yet about 40% of its proteome remains functionally uncharacterized due to the lack of conserved homologous proteins and limited experimental validation of protein function. To begin addressing this gap, we created …

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Giardia's domed ventral disc architecture is essential for attachment and contributes to epithelial barrier disruption

Kari D. Hagen, Christopher Nosala, Antonia Müller, Nicholas A. Hilton et autres

trophozoites attach extracellularly to the host gastrointestinal epithelium using a unique microtubule (MT) organelle, the ventral disk. The complex, dome-shaped disk is composed of microribbon-cross-bridge (MR-CB) protein complexes scaffolded onto a spiral MT array. Attachment is dynamic and reversible, facilitating parasite contact …

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Arginine metabolism has a pivotal function for the encystation of Giardia duodenalis

Christian Klotz, Ricarda Leisering, Kari D. Hagen, Hannah Starcevich et autres

Abstract Arginine metabolism plays a key role in the energy metabolism of the intestinal parasite Giardia duodenalis , an amitochondrial protozoan that infects humans and animals and causes significant morbidity. An arginine deiminase (ADI) has been implicated in virulence, but it is …

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Harnessing the power of new genetic tools to illuminate Giardia biology and pathogenesis

Kari D. Hagen, Christopher J. S. Hart, Shane G. McInally, Scott C. Dawson

Giardia is a prevalent single-celled microaerophilic intestinal parasite causing diarrheal disease and significantly impacting global health. Double diploid (essentially tetraploid) Giardia trophozoites have presented a formidable challenge to the development of molecular genetic tools to interrogate gene function. High sequence divergence and …

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Efficient CRISPR/Cas9-mediated gene disruption in the tetraploid protist Giardia intestinalis

Vendula Horáčková, Luboš Voleman, Kari D. Hagen, Markéta Petrů et autres

CRISPR/Cas9-mediated genome editing has become an extremely powerful technique used to modify gene expression in many organisms, including parasitic protists. Giardia intestinalis , a protist parasite that infects approximately 280 million people around the world each year, has been eluding the use …

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Author response for "Efficient CRISPR/Cas9-mediated gene disruption in the tetraploid protist Giardia intestinalis"

Vendula Horáčková, Luboš Voleman, Kari D. Hagen, Markéta Petrů et autres

Supplementary Table 1 from Efficient CRISPR/Cas9-mediated gene disruption in the tetraploid protist Giardia intestinalis

Vendula Horáčková, Luboš Voleman, Kari D. Hagen, Markéta Petrů et autres

Mass spectrometry datasets comparing protein levels in KO cells and in corresponding control cell lines. Each list stands for one knock-out strain. Proteins corresponding to knocked-out/knocked-down genes are highlighted in red in each list.

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Supplementary Table 2 from Efficient CRISPR/Cas9-mediated gene disruption in the tetraploid protist Giardia intestinalis

Vendula Horáčková, Luboš Voleman, Kari D. Hagen, Markéta Petrů et autres

List of primers used in the study.

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Disc-associated proteins mediate the unusual hyperstability of the ventral disc in Giardia lamblia

Christopher Nosala, Kari D. Hagen, Nicholas A. Hilton, Tiffany M. Chase et autres

, a widespread parasitic protozoan, attaches to the host gastrointestinal epithelium by using the ventral disc, a complex microtubule (MT) organelle. The 'cup-like' disc is formed by a spiral MT array that scaffolds numerous disc-associated proteins (DAPs) and higher-order protein complexes. In …

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Recent advances in functional research in Giardia intestinalis

Aaron R. Jex, Staffan G. Svärd, Kari D. Hagen, Hannah Starcevich et autres

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Microtubule organelles in Giardia

Kari D. Hagen, Shane G. McInally, Nicholas D. Hilton, Scott C. Dawson

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Robust and stable transcriptional repression in Giardia using CRISPRi

Shane G. McInally, Kari D. Hagen, Christopher Nosala, Joseph C. Williams et autres

Giardia lamblia is a binucleate protistan parasite causing significant diarrheal disease worldwide. An inability to target Cas9 to both nuclei, combined with the lack of nonhomologous end joining and markers for positive selection, has stalled the adaptation of CRISPR/Cas9-mediated genetic tools for …

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