Accès ouvert
2026
supplementary-materials
OpenAlex
Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud et autres
Supplemental Figure 3 - Glucose is important for bone marrow-derived macrophage polarization and function in vitro. A.) Relative KPC cell survival when co-cultured with M1- or M2-like macrophages in high vs low glucose, assessed via quantification of KPC cell luminescence (similar experiment …
Accès ouvert
2026
supplementary-materials
OpenAlex
Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud et autres
Supplemental Figure 10 - Effects of hyperglycemia and PLX3397 in an orthotopic model of hepatic metastases. A.) Median survival of C57Bl/6J mice with orthotopic hepatic metastases treated with PLX3397 with or without D30 water. Treatment was stopped after 60 days. An asterisk …
Accès ouvert
2026
other
OpenAlex
Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud et autres
Intentional hyperglycemia affects the TME. A, Peripheral glucose levels in tumor-bearing C57Bl/6J mice receiving standard water or D30 water for 2 weeks, measured using an Abbot glucometer. B, Relative intratumoral glucose levels from KPC orthotopic pancreatic tumors in C57Bl/6J mice consuming standard …
Accès ouvert
2026
other
OpenAlex
Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud et autres
Hyperglycemia affects the immune profile of mice receiving macrophage-modulating immunotherapies. GO analysis of tumors from mice receiving standard water or D30 water with or without PLX3397 (A–D). Representative flow cytometry plots and quantitative analyses showing the effects of vehicle control, D30, PLX3397, …
Accès ouvert
2026
other
OpenAlex
Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud et autres
Abstract Pancreatic ductal adenocarcinoma (PDAC) is characterized by an immunosuppressive tumor microenvironment (TME) that limits the efficacy of immunotherapies. Many tumor-suppressive immune cells, including inflammatory macrophage subsets, rely on glycolysis to sustain effector function; however, the pancreatic TME is relatively glucose-limited. In …
Accès ouvert
2026
supplementary-materials
OpenAlex
Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud et autres
Supplemental Figure 8 - Flow cytometric analysis of additional immune and stromal cell infiltration following single and combination treatments in pancreatic tumors. A.) Representative flow cytometry plots illustrating the effects of vehicle control, D30, PLX3397, PLX3397 + D30, PF-4136309, and PF-4136309 + …
Accès ouvert
2026
other
OpenAlex
Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud et autres
High glucose is needed to maintain the metabolic profile of M1-like macrophages in vitro. OCR (A) and ECAR (B) of M1- and M2-like macrophages at differing glucose levels after 48 hours of treatment, assessed via Seahorse extracellular flux analysis. C, OCR of …
Accès ouvert
2026
supplementary-materials
OpenAlex
Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud et autres
Supplemental Figure 11 - Durable response to immunotherapy in a patient with diabetes who developed metastatic disease after a pancreatectomy and progressed on chemotherapy. CA 19-9 (U/mL) values are depicted by the red line. Peripheral glucose levels (mg/dL) are depicted by the …
Accès ouvert
2026
supplementary-materials
OpenAlex
Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud et autres
Supplemental Figure 6 - Flow cytometry gating strategy for immune profiling of pancreatic tumor–bearing mice. Representative flow cytometry plots illustrating the sequential gating strategy used for the identification of lymphoid and myeloid populations in pancreatic tumor samples (indicated as PAN_HG). Briefly, total …
Accès ouvert
2026
supplementary-materials
OpenAlex
Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud et autres
Supplemental Figure 4 - Macrophage-targeting immunotherapies are associated with a protective effect for M1-like macrophage polarization in vitro without impacting macrophage viability. A.) Western blot of M0, M1-, and M2-like macrophages treated with vehicle or PLX3397 (100 nm) in high (25 mM) …
Accès ouvert
2026
supplementary-materials
OpenAlex
Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud et autres
Supplemental Figure 1 - Immune competence is required to restrain pancreatic cancer growth in vivo. A.) Overall survival of mice with different immune backgrounds bearing identical KPC orthotopic pancreatic tumors. NSG mice lack functional T, B, and NK cells and have severely …
Accès ouvert
2026
supplementary-materials
OpenAlex
Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud et autres
Supplemental Figure 12 - Hyperglycemia is associated with an altered immune profile. Representative mIHC and immunofluorescence images of metastatic PDAC tumors obtained from non-hyperglycemic (left) and hyperglycemic (right) patients treated at OHSU. Single stained IHC images (top) depict tumor cells (PanCK), antigen-presenting …