Stephanie Broome

Sustained microglial depletion with CSF1R inhibitor impairs parenchymal plaque development in an Alzheimer’s disease model

Elizabeth E. Spangenberg, Paul L. Severson, Lindsay A. Hohsfield, Joshua D. Crapser et autres

Many risk genes for the development of Alzheimer's disease (AD) are exclusively or highly expressed in myeloid cells. Microglia are dependent on colony-stimulating factor 1 receptor (CSF1R) signaling for their survival. We designed and synthesized a highly selective brain-penetrant CSF1R inhibitor (PLX5622) …

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Amyloid-β 11 C-PiB-PET imaging results from 2 randomized bapineuzumab phase 3 AD trials

Enchi Liu, Mark E. Schmidt, Richard A. Margolin, Reisa Anne Sperling et autres

OBJECTIVE: To evaluate the effects of bapineuzumab on brain β-amyloid (Aβ) burden using (11)C-Pittsburgh compound B ((11)C-PiB)-PET. METHODS: Two phase 3 clinical trials, 1 each in apolipoprotein APOE ε4 carriers and noncarriers, were conducted in patients with mild to moderate Alzheimer disease …

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Immunological screening method to detect specific translation products. 1978.

Stephanie Broome, W Gilbert

A bacterial clone synthesizing proinsulin. 1978.

Lydia Villa‐Komaroff, Argiris Efstratiadis, Stephanie Broome, Peter T. Lomedico et autres

Simplified northern blot hybridization using 5% sodium dodecyl sulfate.

G. Duke Virca, W Northemann, Brian R Shiels, Georg Widera et autres

We describe a simplified Northern blot hybridization procedure. This procedure eliminates the need for many reagents commonly used in RNA hybridization and replaces them with two buffers containing 5% sodium dodecyl sulfate for pre-hybridization, hybridization and all post-hybridization washes.

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The effect of epidermal growth factor, insulin and transferrin on the growth-promoting properties of serum depleted by repeated culture of postimplantation rat embryos

Margaret Kathryn Pratten, Adrian Brooke, Stephanie Broome, Felix Beck

Homologous serum, when repeatedly used for the culture of postimplantation rat embryos, rapidly loses its capacity to support growth and development. Replenishment of the 'exhausted' serum with glucose and vitamins (MEM vitamin concentrate--Flow Laboratories) together with gentle dialysis to remove small molecular …

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Rous sarcoma virus encodes a transcriptional activator

Stephanie Broome, Walter Gilbert

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Biological activity of rat proinsulin synthesized by Escherichia coli

Stephen P. Naber, Lydia Villa‐Komaroff, Stephanie Broome, Aldo A. Rossini et autres

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The synthesis of insulin in bacteria: a model for the production of medically useful proteins in prokaryotic cells.

Lydia Villa‐Komaroff, Stephanie Broome, Stephen P. Naber, Argiris Efstratiadis et autres

THE STRUCTURE AND TRANSCRIPTION OF RAT PREPROINSULIN GENES

Argiris Efstratiadis, Peter T. Lomedico, Nadia A Rosenthal, Richard David Kolodner et autres

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A bacterial clone synthesizing proinsulin.

Lydia Villa‐Komaroff, Argiris Efstratiadis, Stephanie Broome, Peter T. Lomedico et autres

We have cloned double-stranded cDNA copies of a rat preproinsulin messenger RNA in Escherichia coli chi1776, using the unique Pst endonuclease site of plasmid pBR322 that lies in the region encoding amino acids 181-182 of penicillinase. This site was reconstructed by inserting …

Immunological screening method to detect specific translation products.

Stephanie Broome, W Gilbert

We describe a very sensitive method to detect as antigens the presence of specific proteins within phage plaques or bacterial colonies. We coat plastic sheets with antibody molecules, expose the sheet to lysed bacteria so that a released antigen can bind, and …