Alberto Trovato

Quantitative measurement of antibiotic resistance in Mycobacterium tuberculosis reveals genetic determinants of resistance and susceptibility in a target gene approach

Ivan Barilar, Simone Battaglia, Emanuele Borroni, Angela Pires Brandão et autres

The World Health Organization has a goal of universal drug susceptibility testing for patients with tuberculosis. However, molecular diagnostics to date have focused largely on first-line drugs and predicting susceptibilities in a binary manner (classifying strains as either susceptible or resistant). Here, …

de, it, br, gb, us, Afrique du Sud, pe, cn, au, se, ca, in, vn, sg, Madagascar, ch, fr (code pays fourni par la source)

Bedaquiline and clofazimine resistance in Mycobacterium tuberculosis: an in-vitro and in-silico data analysis

Lindsay Sonnenkalb, Joshua James Carter, Andrea Spitaleri, Zamin Iqbal et autres

BACKGROUND: Bedaquiline is a core drug for the treatment of multidrug-resistant tuberculosis; however, the understanding of resistance mechanisms is poor, which is hampering rapid molecular diagnostics. Some bedaquiline-resistant mutants are also cross-resistant to clofazimine. To decipher bedaquiline and clofazimine resistance determinants, we …

de, gb, us, it, in (code pays fourni par la source)

The 2021 WHO catalogue of Mycobacterium tuberculosis complex mutations associated with drug resistance: a genotypic analysis

Paolo Miotto, Claudio U. Köser, Philip W. Fowler, Zamin Iqbal et autres

Background Molecular diagnostics are considered the most promising route to achievement of rapid, universal drug susceptibility testing for Mycobacterium tuberculosis complex (MTBC).We aimed to generate a WHO-endorsed catalogue of mutations to serve as a global standard for interpreting molecular information for drug …

gb, vn, it, us, es, Afrique du Sud, ch, br, in, cn, au, de (code pays fourni par la source)

Draft Genome Sequences of 77 Endemic Multidrug-Resistant Mycobacterium tuberculosis Strains of SIT41 (TUR) Spoligotype from Bulgaria

Stefan Panaiotov, Vladimir Tolchkov, Yordan Hodzhev, Borislava Tsafarova et autres

Sequences of multidrug-resistant (MDR) Mycobacterium tuberculosis strains are of particular interest to study the molecular mechanisms of drug resistance evolution. Here, we report the draft genome sequences of 77 endemic multidrug-resistant Mycobacterium tuberculosis strains of SIT41 (TUR) spoligotype from Bulgaria. SIT41 spoligotype …

bg, it (code pays fourni par la source)

Drug susceptibility testing of Mycobacterium tuberculosis using next generation sequencing and Mykrobe software

Vladimir Tolchkov, Yordan Hodzhev, Borislava Tsafarova, Elizabeta Bachiyska et autres

Introduction. Mycobacterium tuberculosis is the causative agent of tuberculosis. Drug susceptibility testing is performed by phenotypic and molecular tests. Commonly used for phenotypic drug susceptibility testing is the automated BACTEC system in a liquid culture medium. Drug susceptibility by line probe molecular …

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Detection of NDM-1/5 and OXA-48 co-producing extensively drug-resistant hypervirulent Klebsiella pneumoniae in Northern Italy

Giovanni Lorenzin, Floriana Gona, Simone Battaglia, Andrea Spitaleri et autres

Using a hybrid long-read sequencing approach, we aimed to fully characterise four extensively-drug resistant (XDR) hypervirulent Klebsiella pneumoniae isolates, one of which represented the first strain isolated in Italy co-expressing NDM-1/5 and OXA-48 carbapenemases. Whole-genome sequencing was performed using Illumina and Oxford …

it (code pays fourni par la source)

Anti-tuberculosis drug resistance in Slovakia, 2018–2019: The first whole-genome epidemiological study

Matúš Dohál, Věra Dvořáková, Miluše Šperková, Igor Porvazník et autres

OBJECTIVE: to antituberculosis drugs poses a major threat to global public health. Whole genome sequencing (WGS) is an increasingly preferred method in the diagnostics and monitoring of the transmission dynamics of resistant forms of tuberculosis (TB). The aim of the study was …

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In vivomicroevolution ofMycobacterium tuberculosisand transient emergence ofatpE_Ala63Pro mutation during treatment in a pre-XDR TB patient

Arash Ghodousi, Alamdar Hussain Rizvi, Faisal Masood Khanzada, Nasim Akhtar et autres

This letter describes microevolution of a pre-XDR MTB strain isolated from a pulmonary TB patient over an 18-month exposure to BDQ. MDR-TB therapies with BDQ require a functional background regimen to prevent emergence of additional resistance.https://bit.ly/3D05qT9

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Performance of the GenoType MTBDRsl V 2.0 for detecting second-line drugs resistance of Mycobacterium tuberculosis isolates in Tunisia

Imen Bouzouita, H. Draoui, Andrea Maurizio Cabibbe, Leila Essalah et autres

Tunisie, Maroc, it (code pays fourni par la source)

Epidemiological cutoff values for a 96-well broth microdilution plate for high-throughput research antibiotic susceptibility testing of M. tuberculosis

Philip W. Fowler, Ivan Barilar, Simone Battaglia, Emanuele Borroni et autres

Abstract Drug susceptibility testing of M. tuberculosis is rooted in a binary susceptible/resistant paradigm. Whilst there are considerable advantages in measuring the minimum inhibitory concentrations (MICs) of a panel of drugs for an isolate it is n ecessary to measure the epidemiological …

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Acquisition of Cross-Resistance to Bedaquiline and Clofazimine following Treatment for Tuberculosis in Pakistan

Arash Ghodousi, Alamdar Hussain Rizvi, Aiman Baloch, Abdul Ghafoor et autres

Aims and objectives: In the present study, we report on the first seven cases of acquired bedaquiline and clofazimine resistance identified in Pakistan. The study was conducted within the framework of a retrospective surveillance project to monitor the acquisition of resistance to …

Mycobacterium helveticum sp. nov., a novel slowly growing mycobacterial species associated with granulomatous lesions in adult swine

Giovanni Ghielmetti, Giuliana Rosato, Alberto Trovato, Ute Friedel et autres

The occurrence of nontuberculous mycobacteria in different hosts and their implication as obligate or opportunistic pathogens remain mainly unclear. Mycobacteriosis in pigs is usually associated with members of the Mycobacterium avium complex and, in particular, with ‘ Mycobacterium avium subsp. hominissuis ’. …

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