Paula Ordonez

Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells

Paula Ordonez, Kate N. Bishop, Jonathan P. Stoye, Harriet Cordelia Theed Groom

Sterile α-motif/histidine-aspartate domain-containing protein 1 (SAMHD1) inhibits replication of HIV-1 in quiescent myeloid cells. U937 cells are widely used as a convenient cell system for analyzing SAMHD1 activity due to a low level of SAMHD1 RNA expression, leading to undetectable endogenous protein …

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Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells

Paula Ordonez, Kate N. Bishop, Jonathan P. Stoye, Harriet Cordelia Theed Groom

Sterile α-motif/histidine-aspartate domain-containing protein 1 (SAMHD1) inhibits replication of HIV-1 in quiescent myeloid cells. U937 cells are widely used as a convenient cell system for analyzing SAMHD1 activity due to a low level of SAMHD1 RNA expression, leading to undetectable endogenous protein …

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Author Correction: Scalable and robust SARS-CoV-2 testing in an academic center

Jim Aitken, Karen V. Ambrose, Sam Barrell, Rupert C. L. Beale et autres

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Scalable and robust SARS-CoV-2 testing in an academic center

Jim Aitken, Karen V. Ambrose, Sam Barrell, Rupert C. L. Beale et autres

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SAMHD1 enhances nucleoside-analogue efficacy against HIV-1 in myeloid cells

Paula Ordonez, Simone Kunzelmann, Harriet C. T. Groom, Melvyn W. Yap et autres

SAMHD1 is an intracellular enzyme that specifically degrades deoxynucleoside triphosphates into component nucleoside and inorganic triphosphate. In myeloid-derived dendritic cells and macrophages as well as resting T-cells, SAMHD1 blocks HIV-1 infection through this dNTP triphosphohydrolase activity by reducing the cellular dNTP pool …

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Cytotoxic effects of NF-κB inhibitors in combination with anti-herpes agents on Epstein-Barr virus-positive gastric carcinoma in vitro

Paula Ordonez, Athira Nandakumar, Chihaya Koriyama, Megumi Yamomoto et autres

Epstein-Barr virus (EBV) infection in tumor cells is usually restricted to the latent form, indicating that the induction of viral lytic infection may present a novel approach for the treatment of EBV‑associated tumors. By contrast, EBV lytic replication is inhibited by high‑levels …

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Phospho-dependent Regulation of SAMHD1 Oligomerisation Couples Catalysis and Restriction

Laurence H. Arnold, Harriet C. T. Groom, Simone Kunzelmann, David Schwefel et autres

SAMHD1 restricts HIV-1 infection of myeloid-lineage and resting CD4+ T-cells. Most likely this occurs through deoxynucleoside triphosphate triphosphohydrolase activity that reduces cellular dNTP to a level where reverse transcriptase cannot function, although alternative mechanisms have been proposed recently. Here, we present combined …

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Integrin alphavbeta5 identifies human corneal epithelial stem cells

Nick Di Girolamo, Paula Ordonez, Sharron Chow

Abstract Purpose Stem cell (SC) therapy is the main treatment modality to restore vision in patients with limbal stem cell deficiency. If corneolimbal epithelial stem cells (LESC) can be readily identified, isolated and maintained ex vivo, better quality grafts can be generated …

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Human limbal epithelial progenitor cells express αvβ5-integrin and the interferon-inducible chemokine CXCL10/IP-10

Paula Ordonez, Sharron Chow, Denis Wakefield, Nick Di Girolamo

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Vitronectin and its Receptor αvβ5 in Limbal Epithelial Stem Cell Maintenance

Nick Di Girolamo, Paula Ordonez, Sharron Chow, Denis Wakefield

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Anti-Human Immunodeficiency Virus Type 1 Activity of Novel 6-Substituted 1-Benzyl-3-(3,5-Dimethylbenzyl)Uracil Derivatives

Paula Ordonez, Takayuki Hamasaki, Yohei Isono, Norikazu Sakakibara et autres

Nonnucleoside reverse transcriptase (RT) inhibitors (NNRTIs) are important components of current combination therapies for human immunodeficiency virus type 1 (HIV-1) infection. In screening of chemical libraries, we found 6-azido-1-benzyl-3-(3,5-dimethylbenzyl)uracil (AzBBU) and 6-amino-1-benzyl-3-(3,5-dimethylbenzyl)uracil (AmBBU) to be highly active and selective inhibitors of HIV-1 …

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Limbal Epithelial Stem Cells: Role of the Niche Microenvironment

Paula Ordonez, Nick Di Girolamo

The cornea contains a reservoir of self-regenerating epithelial cells that are essential for maintaining its transparency and good vision. The study of stem cells in this functionally important organ has grown over the past four decades, partly due to the ease with …

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