Mangaiarkarasi Asokan

FcγR binding differentially contributes to protection by two human monoclonal antibodies targeting Plasmodium falciparum circumsporozoite protein

Neville K. Kisalu, Lais Da Silva Pereira, Jonathan D. Herman, Mangaiarkarasi Asokan et autres

Antibodies mediate protection against a wide range of pathogens through binding and neutralizing the pathogen or through Fc-mediated effector functions. Human monoclonal antibodies (mAbs) CIS43LS and L9LS show high-affinity binding targeting distinct regions on the Plasmodium falciparum circumsporozoite protein (PfCSP) and are …

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Editorial Expression of Concern: Exceptional molecular and coreceptor-requirement properties of molecular clones isolated from an human immunodeficiency virus Type-1 subtype C infection

Nagadenahalli B. Siddappa, Mangaiarkarasi Asokan, Aruna V. Mahendarkar, Krishnamurthy Kumar Anand et autres

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Administration of anti-HIV-1 broadly neutralizing monoclonal antibodies with increased affinity to Fcγ receptors during acute SHIVAD8-EO infection

Joana Dias, Giulia Fabozzi, Slim Fourati, Xuejun Chen et autres

Abstract Anti-HIV-1 broadly neutralizing antibodies (bNAbs) have the dual potential of mediating virus neutralization and antiviral effector functions through their Fab and Fc domains, respectively. So far, bNAbs with enhanced Fc effector functions in vitro have only been tested in NHPs during …

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Immunogenicity of SARS-CoV-2 vaccines BBV152 (COVAXIN®) and ChAdOx1 nCoV-19 (COVISHIELD™) in seronegative and seropositive individuals in India: a multicentre, nonrandomised observational study

Mangaiarkarasi Asokan, Roshni Florina Joan, Sudhir Babji, Girish Dayma et autres

Background: There are limited global data on head-to-head comparisons of vaccine platforms assessing both humoral and cellular immune responses, stratified by pre-vaccination serostatus. The COVID-19 vaccination drive for the Indian population in the age group 18-45 years began in April 2021 when …

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Polyfunctional CD4 T-cells correlating with neutralising antibody is a hallmark of COVISHIELDTM and COVAXIN® induced immunity in COVID-19 exposed Indians

Srabanti Rakshit, Sudhir Babji, Chaitra Parthiban, Ramya Madhavan et autres

(CS) and COVAXIN® (CO) in a pre-exposed population is only recently being discovered. We addressed this issue in subjects who received their primary series of vaccination between November 2021 and January 2022. Both vaccines are capable of strongly boosting Wuhan Spike-specific neutralising …

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Improved pharmacokinetics of HIV-neutralizing VRC01-class antibodies achieved by reduction of net positive charge on variable domain

Young Do Kwon, Amarendra Pegu, Eun Sung Yang, Baoshan Zhang et autres

The amino-acid composition of the immunoglobulin variable region has been observed to impact antibody pharmacokinetics (PK). Here, we sought to improve the PK of the broad HIV−1-neutralizing VRC01-class antibodies, VRC07-523LS and N6LS, by reducing the net positive charge in their variable domains. …

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Bispecific antibody CAP256.J3LS targets V2-apex and CD4-binding sites with high breadth and potency

Baoshan Zhang, Jason Gorman, Young Do Kwon, Amarendra Pegu et autres

Antibody CAP256-VRC26.25 targets the second hypervariable region (V2) at the apex of the HIV envelope (Env) trimer with extraordinary neutralization potency, although less than optimal breadth. To improve breadth, we linked the light chain of CAP256V2LS, an optimized version of CAP256-VRC26.25 currently …

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Interim results from comparison of immune responses elicited by an inactivated and a vectored SARS-CoV-2 vaccine in seronegative and seropositive participants in India

Mangaiarkarasi Asokan, Roshni Florina Joan, Sudhir Babji, Girish Dayma et autres

Abstract Background There are limited global data on head-to-head comparisons of vaccine platforms assessing both humoral and cellular immune responses, stratified by pre-vaccination serostatus. The COVID-19 vaccination drive for the Indian population in the 18 to 45-year age-group began in April 2021 …

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Engineering of HIV-1 neutralizing antibody CAP256V2LS for manufacturability and improved half life

Baoshan Zhang, Deepika Gollapudi, Jason Gorman, Sijy O’Dell et autres

The broadly neutralizing antibody (bNAb) CAP256-VRC26.25 has exceptional potency against HIV-1 and has been considered for clinical use. During the characterization and production of this bNAb, we observed several unusual features. First, the antibody appeared to adhere to pipette tips, requiring tips …

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Engineering of HIV-1 neutralizing antibody CAP256V2LS for manufacturability and improved half life

Baoshan Zhang, Deepika Gollapudi, Jason Gorman, Sijy O’Dell et autres

Abstract The broadly neutralizing antibody (bNAb) CAP256-VRC26.25 has exceptional potency against HIV-1 and has been considered for clinical use. During the characterization and production of this bNAb, we observed several unusual features. First, the antibody appeared to adhere to pipette tips, requiring …

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Potent anti-viral activity of a trispecific HIV neutralizing antibody in SHIV-infected monkeys

Amarendra Pegu, Ling Xu, Megan Demouth, Giulia Fabozzi et autres

Broadly neutralizing antibodies (bNAbs) represent an alternative to drug therapy for the treatment of HIV-1 infection. Immunotherapy with single bNAbs often leads to emergence of escape variants, suggesting a potential benefit of combination bNAb therapy. Here, a trispecific bNAb reduces viremia 100- …

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Concordance of immunological events between intrarectal and intravenous SHIVAD8-EO infection when assessed by Fiebig-equivalent staging

Joana Dias, Giulia Fabozzi, Kylie March, Mangaiarkarasi Asokan et autres

Primary HIV-1 infection can be classified into six Fiebig stages based on virological and serological laboratory testing, whereas simian-HIV (SHIV) infection in nonhuman primates (NHPs) is defined in time post-infection, making it difficult to extrapolate NHP experiments to the clinics. We identified …

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