A. Mazzaglia

Development and validation of a species-specific qPCR assay for the detection of Diaporthe amygdali in woody plant tissues

Antonella Cardacino, Federico Brugneti, Mattia Onofri, A. Mazzaglia et autres

Diaporthe amygdali, a major causal agent of twig canker in Prunus species, poses a significant threat to fruit production, highlighting the need for rapid and reliable detection tools. In this study, a SYBR Green-based qPCR assay targeting the β-tubulin gene was developed …

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Bárbara Soledad Defea, A. Mazzaglia, Susana L. Paradell, G. Lucero et autres

This study aimed to identify potential vectors of the bacterium Xylella fastidiosa in olive orchards in La Rioja Province, Argentina. Hoppers (Hemiptera: Auchenorrhyncha) were collected during the spring of 2019 from localities with a high incidence of this pathogen in olive trees …

Olive Anthracnose: Current State of the Art, Global Distribution and Associated Colletotrichum Species

Federico Brugneti, Luca Rossini, Cristian Silvestri, A. Mazzaglia et autres

ABSTRACT The olive tree ( Olea europaea ) is one of the most important perennial crops worldwide for both the nutritional value of its products and for the economic revenue. Its continuous expansion into new geographic regions is also broadening the range …

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Clearing the Noise: Seasonal Dynamics of Endophytic Bacteria in Fagus sylvatica Leaves Revealed by Application of PNA Clamps

Irene Giubilei, Silvia Turco, Antonella Cardacino, Lovely Mahawar et autres

The characterization of the seasonal dynamics of endophytic bacteria in beech leaves can be hindered by co-amplification of chloroplast and mitochondrial plant DNA. This study applies established peptide nucleic acid (PNA) clamps to suppress host-derived amplification while resolving bacterial succession across the …

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Primer registro de potenciales vectores (Hemiptera: Auchenorrhyncha) de Xylella fastidiosa en olivares de Argentina

Bárbara Soledad Defea, A. Mazzaglia, Susana L. Paradell, G. Lucero et autres

This study aimed to identify potential vectors of the bacterium Xylella fastidiosa in olive orchards in La Rioja Province, Argentina. Hoppers (Hemiptera: Auchenorrhyncha) were collected during the spring of 2019 from localities with a high incidence of this pathogen in olive trees …

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In Vitro Antagonistic Activity of Trichoderma gamsii Isolated From Hazelnut Twigs Against Colletotrichum spp.

Federico Brugneti, Antonella Cardacino, Giuseppina Fanelli, Sara Rinalducci et autres

ABSTRACT Trichoderma species are widely recognised for their antagonistic activity against phytopathogenic fungi and for their ability to promote plant growth, hence representing promising candidates for sustainable crop protection. In this study, a Trichoderma gamsii isolate was recovered from the phyllosphere of …

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Harnessing Genomics of Diaporthe amygdali for Improved Control of Peach Twig Canker and Shoot Blight (TCSB)

Silvia Turco, Federico Brugneti, Antonella Cardacino, A. Mazzaglia

In recent years, symptoms of Twig Cankers and Shoot Blight (TCSB) have re-emerged in several Italian peach orchards, particularly within key production areas of the Emilia-Romagna region. The fungal pathogen Diaporthe amygdali is recognized as the primary causal agent of TCSB, leading …

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Peach Buds’ Microbiome Profiling Reveals Cultivar-Specific Signatures Associated with TCSB Susceptibility

Antonella Cardacino, Taner Tastekin, Federico Brugneti, Marco Cirilli et autres

The plant microbiome plays a pivotal role in host development and resilience against biotic and abiotic stresses. In perennial crops like peach, microbial communities inhabiting dormant buds—critical yet vulnerable organs—may influence disease outcomes and plant fitness. This study characterized the bacterial and …

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“The Last of Them”: Entomopathogenic Effect of Akanthomyces muscarius on the Scale Insect Pest Toumeyella parvicornis Under Laboratory Conditions, a Potential Biological Control Candidate

Nicolò Di Sora, Silvia Turco, Federico Brugneti, Flavia Isoli et autres

Entomopathogenic fungi are valuable alternatives to traditional agrochemicals, providing more sustainable crop protection. Akanthomyces spp. is acknowledged in biocontrol practices, especially in controlling aphids, thrips, whiteflies, and mites; however, its efficacy on soft scale insects is still poorly known. This study investigates …

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Occurrence of Neofusicoccum parvum Associated With Dieback of Ailanthus altissima in Italy

Irene Giubilei, Mounira Inas Drais, Danilo Cos, A. Mazzaglia

ABSTRACT The invasive deciduous tree Ailanthus altissima, native to China, has rapidly spread worldwide and is now considered one of the most ecologically disruptive species. During a survey carried out in 2024 in Rome province, trees exhibiting severe dieback symptoms were documented, …

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Elucidation of “Twig Canker and Shoot Blight” ( TCSB ) in Peach Caused by Diaporthe amygdali in the North of Italy in Emilia‐Romagna

Federico Brugneti, Luca Rossini, Marco Cirilli, Alessia Nardecchia et autres

Prunus persica (peach) is a major fruit species in global agriculture, with Spain and Italy being the leading producers in the European Union. As agriculture is subject to increasing pressure to reduce agrochemicals, effective management of diseases such as twig canker and …

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Survival of plant pathogens during composting of bio-waste: a case study for oomycetes and fungi

Leonardo Guidoni, Mounira Inas Drais, Silvia Turco, A. Mazzaglia et autres

European countries have implemented national strategies to reduce the use of peat in horticulture due to its environmental impact. Studies demonstrated the possibility of reducing peat consumption by using compost as a substitute without affecting the growth and development of potted horticulture …

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