Jan Parker‐Thornburg

Colony Management (Genetics)

Belén Pintado, Jan Parker‐Thornburg, James Bussell

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TT2022 meeting report on the 17th Transgenic Technology meeting in Helsinki, Finland

Jan Parker‐Thornburg, Fernando J. Benavides, William Shawlot, Elena McBeath

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Mammary-specific expression of Trim24 establishes a mouse model of human metaplastic breast cancer

Vrutant V. Shah, Aundrietta D. Duncan, Shiming Jiang, Sabrina A. Stratton et autres

Abstract Conditional overexpression of histone readerTripartitemotif containing protein 24 (TRIM24) in mouse mammary epithelia (Trim24COE) drives spontaneous development of mammary carcinosarcoma tumors, lacking ER, PR and HER2. Human carcinosarcomas or metaplastic breast cancers (MpBC) are a rare, chemorefractory subclass of triple-negative breast …

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Response to correspondence on “Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation”

Channabasavaiah B. Gurumurthy, Aidan R. O’Brien, Rolen M. Quadros, John Henry Adams et autres

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The Long Noncoding RNA CCAT2 Induces Chromosomal Instability Through BOP1-AURKB Signaling

Baoqing Chen, Mihnea Paul Dragomir, Linda Fabris, Recep Bayraktar et autres

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Rapid Evaluation of CRISPR Guides and Donors for Engineering Mice

Elena McBeath, Jan Parker‐Thornburg, Yuka Fujii, Neeraj K. Aryal et autres

Although the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/ CRISPR associated protein 9 (Cas9) technique has dramatically lowered the cost and increased the speed of generating genetically engineered mice, success depends on using guide RNAs and donor DNAs which direct efficient knock-out …

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Genotyping Genetically Modified (GM) Mice

Neeraj K. Aryal, Jan Parker‐Thornburg

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Breeding Strategies for Genetically Modified Mice

Jan Parker‐Thornburg

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Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

Channabasavaiah B. Gurumurthy, Aidan R. O’Brien, Rolen M. Quadros, John Henry Adams et autres

BACKGROUND: CRISPR-Cas9 gene-editing technology has facilitated the generation of knockout mice, providing an alternative to cumbersome and time-consuming traditional embryonic stem cell-based methods. An earlier study reported up to 16% efficiency in generating conditional knockout (cKO or floxed) alleles by microinjection of …

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Essential Roles of Tbr1 in the Formation and Maintenance of the Orientation-Selective J-RGCs and a Group of OFF-Sustained RGCs in Mouse

Takae Kiyama, Ye Long, Ching-Kang Chen, Christopher M. Whitaker et autres

In the mouse retina, more than 30 retinal ganglion cell (RGC) subtypes have been classified based on a combined metric of morphological and functional characteristics. RGCs arise from a common pool of retinal progenitor cells during embryonic stages and differentiate into mature …

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Different Munc18 proteins mediate baseline and stimulated airway mucin secretion

Ana Maria Jaramillo, Lucia Piccotti, Walter V. Velasco, Anna Sofia Huerta Delgado et autres

Airway mucin secretion is necessary for ciliary clearance of inhaled particles and pathogens but can be detrimental in pathologies such as asthma and cystic fibrosis. Exocytosis in mammals requires a Munc18 scaffolding protein, and airway secretory cells express all 3 Munc18 isoforms. …

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Additional file 5: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

Channabasavaiah B. Gurumurthy, Aidan O’Brien, Rolen M. Quadros, John Henry Adams et autres

Table S5. The details and the results of the 330 unique loci attempted for ssODN knock-in projects (i.e. point mutation knock-in or short-tag insertion projects). (XLSX 14 kb)