Eleanor C. Ory

Hydrogen Peroxide Induces α-Tubulin Detyrosination and Acetylation and Impacts Breast Cancer Metastatic Phenotypes

Megan B. Stemberger, Julia A. Ju, Keyata N. Thompson, Trevor J. Mathias et autres

Levels of hydrogen peroxide are highly elevated in the breast tumor microenvironment compared to normal tissue. Production of hydrogen peroxide is implicated in the mechanism of action of many anticancer therapies. Several lines of evidence suggest hydrogen peroxide mediates breast carcinogenesis and …

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Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor Cells

Katarina T. Chang, Keyata N. Thompson, Stephen J. P. Pratt, Julia A. Ju et autres

Cytoskeletal remodeling in circulating tumor cells (CTCs) facilitates metastatic spread. Previous oncology studies examine sustained aberrant calcium (Ca2+) signaling and cytoskeletal remodeling scrutinizing long-term phenotypes such as tumorigenesis and metastasis. The significance of acute Ca2+ signaling in tumor cells that occur within …

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Tubulin Carboxypeptidase Activity Promotes Focal Gelatin Degradation in Breast Tumor Cells and Induces Apoptosis in Breast Epithelial Cells That Is Overcome by Oncogenic Signaling

Trevor J. Mathias, Julia A. Ju, Rachel Lee, Keyata N. Thompson et autres

Post-translational modifications (PTMs) of the microtubule network impart differential functions across normal cell types and their cancerous counterparts. The removal of the C-terminal tyrosine of α-tubulin (deTyr-Tub) as performed by the tubulin carboxypeptidase (TCP) is of particular interest in breast epithelial and …

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Microtubule disruption reduces metastasis more effectively than primary tumor growth

Keyata N. Thompson, Julia A. Ju, Eleanor C. Ory, Stephen J. P. Pratt et autres

Clinical cancer imaging focuses on tumor growth rather than metastatic phenotypes. The microtubule-depolymerizing drug, Vinorelbine, reduced the metastatic phenotypes of microtentacles, reattachment and tumor cell clustering more than tumor cell viability. Treating mice with Vinorelbine for only 24 h had no significant …

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Microtentacle Formation in Ovarian Carcinoma

Jocelyn C. Reader, Cong Fan, Eleanor C. Ory, Julia A. Ju et autres

BACKGROUND: The development of chemoresistance to paclitaxel and carboplatin represents a major therapeutic challenge in ovarian cancer, a disease frequently characterized by malignant ascites and extrapelvic metastasis. Microtentacles (McTNs) are tubulin-based projections observed in detached breast cancer cells. In this study, we …

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Lipid tethering of breast tumor cells reduces cell aggregation during mammosphere formation

Lekhana Bhandary, Patrick C. Bailey, Katarina T. Chang, Karen F. Underwood et autres

Mammosphere assays are widely used in vitro to identify prospective cancer-initiating stem cells that can propagate clonally to form spheres in free-floating conditions. However, the traditional mammosphere assay inevitably introduces cell aggregation that interferes with the measurement of true mammosphere forming efficiency. …

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Microtubule Disruption Reduces Metastasis More Effectively Than Primary Tumor Growth

Keyata N. Thompson, Julia A. Ju, Eleanor C. Ory, Stephen J. P. Pratt et autres

Abstract Background: Clinical breast cancer imaging inevitably focuses on tumor growth rather than the metastatic dissemination that is a greater challenge for patient survival. Emerging preclinical evidence indicates that chemotherapy can elevate levels of circulating tumor cells (CTCs) and increase metastatic recurrence. …

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Mechanoactivation of NOX2-generated ROS elicits persistent TRPM8 Ca 2+ signals that are inhibited by oncogenic KRas

Stephen J. P. Pratt, Rachel Lee, Katarina T. Chang, Erick O. Hernández‐Ochoa et autres

Changes in the mechanical microenvironment and mechanical signals are observed during tumor progression, malignant transformation, and metastasis. In this context, understanding the molecular details of mechanotransduction signaling may provide unique therapeutic targets. Here, we report that normal breast epithelial cells are mechanically …

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Organizing A K 12 Ai Curriculum Using Philosophy Of The Mind

Nalini Bhushan, Glenn W. Ellis, Eleanor C. Ory

High school teams competing in the FIRST Robotics Competition and students of all ages building Lego robots have become increasingly common in K-12 education.Although robotics can be an excellent means to introduce technology and engineering design into the classroom, the scope of …

Abstract 2575: Mechanoactivation of NOX2-generated ROS elicits persistent TRPM8 Ca2+ signals that are inhibited by oncogenic KRas

Stephen J. P. Pratt, Rachel Lee, Erick O. Hernández‐Ochoa, Eleanor C. Ory et autres

Abstract Changes in the mechanical microenvironment and mechanical signals are observed during tumor progression, malignant transformation, and metastasis. In this context, understanding the molecular details of mechanotransduction signaling may provide unique therapeutic targets. Here we report that normal breast epithelial cells are …

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Abstract B68: Characterization of microtentacle phenotype and function in ovarian carcinomas

Cong Fan, Sulan Wu, Jocelyn C. Reader, Eleanor C. Ory et autres

Abstract Ovarian carcinomas are categorized into five major histotypes, each characterized by distinct differences in grade at diagnosis, presentation with metastatic disease, and clinical responses to treatments. Microtentacles (McTNs), microtubule-based extensions of the plasma membrane, were initially described on cells of nongynecologic …

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Partial thermal imidization of polyelectrolyte multilayer cell tethering surfaces (TetherChip) enables efficient cell capture and microtentacle fixation for circulating tumor cell analysis

Julia A. Ju, Cornell J. Lee, Keyata N. Thompson, Eleanor C. Ory et autres

The technical challenges of imaging non-adherent tumor cells pose a critical barrier to understanding tumor cell responses to the non-adherent microenvironments of metastasis, like the bloodstream or lymphatics. In this study, we optimized a microfluidic device (TetherChip) engineered to prevent cell adhesion …

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