Fuping Nie

Biomass-derived carbon nanodots with pH-operated LAMP switching for ultrasensitive DNA detection

Keyi Long, Wenxi Hu, Fuping Nie, Mei Yang et autres

th, cn (code pays fourni par la source)

A novel quantitative real-time PCR with the GAPDH reference gene for peste des petits ruminants

Y. E. Shi, Diangang Han, J. Li, L Ye et autres

Peste des petits ruminants (PPR) is a serious acute, highly contagious disease caused by the peste des petits ruminants virus (PPRV). This study aims to establish a qRT-PCR assay with an internal amplification control for the rapid and accurate detection of PPRV. …

cn (code pays fourni par la source)

Label-Free and DNAzyme-Mediated Biosensor with a High Signal-to-Noise Ratio for a Lumpy Skin Disease Virus Assay

Gaihua Cao, Nannan Yang, Jun Yang, Jiali Li et autres

Lumpy skin disease virus (LSDV) is a severe and highly contagious form of cowpox. As LSDV continues to mutate and there is no vaccine and treatment in nonendemic countries, early detection of LSDV becomes an important basis for epidemic prevention and control, …

cn (code pays fourni par la source)

Dual-mode biosensors for ultrasensitive detection of Legionella pneumophila using CRISPR/Cas12a integrated recombinase polymerase amplification

Yue Qiu, Gaihua Cao, Meimei Shi, Shiying Zhou et autres

cn (code pays fourni par la source)

Completely Free from PAM Limitations: Asymmetric RPA with CRISPR/Cas12a for Nucleic Acid Assays

Gaihua Cao, Nannan Yang, Yifan Xiong, Meimei Shi et autres

Experimentally, Cas12a can recognize multiple protospacer adjacent motif (PAM) sequences and is not restricted to the “TTTN”. However, the application of the CRISPR/Cas12a system is still limited by the PAM for double-stranded DNA (dsDNA). Here, we developed asymmetric RPA (Asy-RPA) to completely …

cn (code pays fourni par la source)

TaqMan real-time RT‒PCR assay with an internal amplification control for rapid detection of Bluetongue virus

CHAOLIN CHEN, Diangang Han, Jing Li, L. Ye et autres

Bluetongue (BT) is an arbovirus disease caused by Bluetongue virus (BTV), which has spread all over the world. In this study, a qRT-PCR assay with an internal amplification control was established for preventing false-negative results in Bluetongue virus (BTV) detection. The primers …

cn (code pays fourni par la source)

Field-friendly and ultra-fast detection platform without nucleic acid extraction for virus detection

Gaihua Cao, Yifan Xiong, Yue Qiu, Jun Yang et autres

cn, th (code pays fourni par la source)

Development of the isothermal recombinase polymerase amplification assays for rapid detection of the genus Capripoxvirus

Libing Liu, Jinfeng Wang, Fuping Nie, Ruiwen Li et autres

cn (code pays fourni par la source)

The End of the Gray Zone: One-Tube Nested Recombinase Polymerase Amplification with Ultrahigh Signal-to-Noise Ratio for Precisely Detecting and Surveilling Viruses

Gaihua Cao, Yifan Xiong, Meimei Shi, Yue Qiu et autres

The samples were difficult to accurately determine positive or negative between 35 and 40 cycles by real-time quantitative PCR (qPCR) as the standard method. Here, we developed one-tube nested recombinase polymerase amplification (ONRPA) technology with CRISPR/Cas12a to overcome this difficulty. ONRPA broke …

cn (code pays fourni par la source)

Current Knowledge on Epizootic Haemorrhagic Disease in China

Jige Xin, Jun Dong, Jing Li, Lingling Ye et autres

Epizootic haemorrhagic disease (EHD) is an infectious, non-contagious viral disease of ruminants caused by epizootic haemorrhagic disease virus (EHDV) and is transmitted by insects of the genus Culicoides. In 2008, EHD was listed on the World Organization for Animal Health (WOAH) list …

cn (code pays fourni par la source)

Multiple accurate and sensitive arrays for Capripoxvirus (CaPV) differentiation

Gaihua Cao, Yifan Xiong, Meimei Shi, Yue Qiu et autres

cn, th (code pays fourni par la source)

Reverse Transcriptase Enzyme-assisted recombinant isothermal amplification with CRISPR/Cpf1 for RNA virus assay

Yifan Xiong, Gaihua Cao, Fuping Nie, Meimei Shi et autres

cn (code pays fourni par la source)