Gary W. Moy

Multiple Modes of Adaptation: Regulatory and Structural Evolution in a Small Heat Shock Protein Gene

Sumaetee Tangwancharoen, Gary W. Moy, Ronald S. Burton

Thermal tolerance is a key determinant of species distribution. Despite much study, the genetic basis of adaptive evolution of thermal tolerance, including the relative contributions of transcriptional regulation versus protein evolution, remains unclear. Populations of the intertidal copepod Tigriopus californicus are adapted …

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Functional diversification of sea urchin ABCC1 (MRP1) by alternative splicing

Tufan Gökirmak, Joseph P. Campanale, Adam M. Reitzel, Lauren E. Shipp et autres

The multidrug resistance protein (MRP) family encodes a diverse repertoire of ATP-binding cassette (ABC) transporters with multiple roles in development, disease, and homeostasis. Understanding MRP evolution is central to unraveling their roles in these diverse processes. Sea urchins occupy an important phylogenetic …

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ABCC5 is required for cAMP-mediated hindgut invagination in sea urchin embryos

Lauren E. Shipp, Rose Z. Hill, Gary W. Moy, Tufan Gökirmak et autres

ATP-binding cassette (ABC) transporters are evolutionarily conserved proteins that pump diverse substrates across membranes. Many are known to efflux signaling molecules and are extensively expressed during development. However, the role of transporters in moving extracellular signals that regulate embryogenesis is largely unexplored. …

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Understanding mechanisms of multidrug resistance with guidance from evolution (994.2)

Tufan Gökirmak, Joseph P. Campanale, Lauren E. Shipp, Gary W. Moy et autres

Cancer accounts annually for over half a million deaths in the U.S. and 7.6 million deaths worldwide. Although effective chemotherapeutics are available for different types of cancers, multidrug resistance (MDR), mediated by ATP‐binding cassette (ABC) transporters, is responsible for the failure of …

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CYTASTERS FROM SEA URCHIN EGGS PARTHENOGENETICALLY ACTIVATED BY PROCAINE

Gary W. Moy, Brigitte Brandriff

We recently demonstrated that unfertilized sea urchin eggs initiate cycles of DNA synthesis and chromosome condensation in response to the local anesthetic procaine hydrochloride (20). Such eggs also develop large cytasters in the central portion of which lie the condensed set of …

Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR) Efflux Transporters

Tufan Gökirmak, Joseph P. Campanale, Lauren E. Shipp, Gary W. Moy et autres

In this study, we cloned, expressed and functionally characterized Stronglycentrotus purpuratus (Sp) ATP-binding cassette (ABC) transporters. This screen identified three multidrug resistance (MDR) transporters with functional homology to the major types of MDR transporters found in humans. When overexpressed in embryos, the …

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Investigating the molecular basis of local adaptation to thermal stress: population differences in gene expression across the transcriptome of the copepod Tigriopus californicus

Sean D. Schoville, Felipe S. Barreto, Gary W. Moy, Anastasia Wolff et autres

BACKGROUND: Geographic variation in the thermal environment impacts a broad range of biochemical and physiological processes and can be a major selective force leading to local population adaptation. In the intertidal copepod Tigriopus californicus, populations along the coast of California show differences …

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A gene-based SNP resource and linkage map for the copepod Tigriopus californicus

Brad R. Foley, Colin G. Rose, Daniel E Rundle, Wai Yie Leong et autres

BACKGROUND: As yet, few genomic resources have been developed in crustaceans. This lack is particularly evident in Copepoda, given the extraordinary numerical abundance, and taxonomic and ecological diversity of this group. Tigriopus californicus is ideally suited to serve as a genetic model …

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Interpopulation patterns of divergence and selection across the transcriptome of the copepod Tigriopus californicus

Felipe S. Barreto, Gary W. Moy, Ronald S. Burton

The accumulation of genetic incompatibilities between isolated populations is thought to lead to the evolution of intrinsic postzygotic isolation. The molecular basis for these mechanisms, however, remains poorly understood. The intertidal copepod Tigriopus californicus provides unique opportunities for addressing mechanistic questions regarding …

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Bindin genes of the Pacific oyster Crassostrea gigas

Gary W. Moy, Victor D. Vacquier

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Extraordinary intraspecific diversity in oyster sperm bindin

Gary W. Moy, Stevan A. Springer, Serean L. Adams, Willie J. Swanson et autres

In free-spawning invertebrates sperm-egg incompatibility is a barrier to mating between species, and divergence of gamete recognition proteins (GRPs) can result in reproductive isolation. Of interest are processes that create reproductive protein diversity within species, because intraspecific variants are potentially involved in …

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Oyster sperm bindin is a combinatorial fucose lectin with remarkable intra-species diversity

Stevan A. Springer, Gary W. Moy, Daniel S. Friend, Willie J. Swanson et autres

Sperm of the oyster, Crassostrea gigas, have ring-shaped acrosomes that, after exocytosis, bind the sperm to the egg vitelline layer. Isolated acrosomal rings contain proteins of various sizes: 35-, 48-, 63-, 75- and 88-kDa. These proteins, called bindins, have identical 24-residue signal …

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