A gene-based SNP resource and linkage map for the copepod Tigriopus californicus
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Le résumé fourni par la source
BACKGROUND: As yet, few genomic resources have been developed in crustaceans. This lack is particularly evident in Copepoda, given the extraordinary numerical abundance, and taxonomic and ecological diversity of this group. Tigriopus californicus is ideally suited to serve as a genetic model copepod and has been the subject of extensive work in environmental stress and reproductive isolation. Accordingly, we set out to develop a broadly-useful panel of genetic markers and to construct a linkage map dense enough for quantitative trait locus detection in an interval mapping framework for T. californicus--a first for copepods. RESULTS: One hundred and ninety Single Nucleotide Polymorphisms (SNPs) were used to genotype our mapping population of 250 F2 larvae. We were able to construct a linkage map with an average intermarker distance of 1.8 cM, and a maximum intermarker distance of 10.3 cM. All markers were assembled into linkage groups, and the 12 linkage groups corresponded to the 12 known chromosomes of T. californicus. We estimate a total genome size of 401.0 cM, and a total coverage of 73.7%. Seventy five percent of the mapped markers were detected in 9 additional populations of T. californicus. Of available model arthropod genomes, we were able to show more colocalized pairs of homologues between T. californicus and the honeybee Apis mellifera, than expected by chance, suggesting preserved macrosynteny between Hymenoptera and Copepoda. CONCLUSIONS: Our study provides an abundance of linked markers spanning all chromosomes. Many of these markers are also found in multiple populations of T. californicus, and in two other species in the genus. The genomic resource we have developed will enable mapping throughout the geographical range of this species and in closely related species. This linkage map will facilitate genome sequencing, mapping and assembly in an ecologically and taxonomically interesting group for which genomic resources are currently under development.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A gene-based SNP resource and linkage map for the copepod Tigriopus californicus
- Date Crossref
- 21/11/2011
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Southern California Department of Biological Sciences pays non établi dans la noticeUniversité ou école supérieure
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Windward Community College pays non établi dans la noticeUniversité ou école supérieure
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Harvard University Department of Organismic and Evolutionary Biology pays non établi dans la noticeUniversité ou école supérieure
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Evolutionary Genomics (United States) pays non établi dans la noticeEntreprise
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Scripps Institution of Oceanography pays non établi dans la noticeStructure de recherche
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University of California San Diego Scripps Institution of Oceanography pays non établi dans la noticeUniversité ou école supérieure
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Windward School pays non établi dans la noticeInstitution
Department of Biological Sciences — University of Southern California, Windward Community College et Department of Organismic and Evolutionary Biology — Harvard University, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.