Jason L. Cantera

A one-pot biplex RPA-Cas assay for sensitive detection of Mycobacterium tuberculosis from tongue swabs

Jason L. Cantera, Mohammad Dehghan Banadaki, Gayathri Nithiananthan, Khushboo Khimani et autres

The global management of tuberculosis (TB) is limited by the infrastructure requirements of current molecular diagnostics and the logistical burdens of sputum-based testing. While isothermal nucleic acid amplification provides a decentralized alternative, its clinical utility is often limited by amplicon carryover contamination …

us (code pays fourni par la source)

Correction: Room temperature CRISPR diagnostics for low-resource settings

Eric A. Nalefski, Selma Sinan, Jason L. Cantera, Remy M. Kooistra et autres

The original version of this Article contained an error in the name of the author Ilya J. Finkelstein, which was incorrectly given as Ilya J. Finklestein.

us (code pays fourni par la source)

Robotic optimization of diagnostic chemistries – Project overview

Caitlin E. Anderson, Jason L. Cantera, Luis Alonzo

Overview of project work to develop application of a robotic liquid handling platform to support diagnostic Research and Development efforts.

us (code pays fourni par la source)

Room temperature CRISPR diagnostics for low-resource settings

Eric A. Nalefski, Selma Sinan, Jason L. Cantera, Remy M. Kooistra et autres

Maintaining elevated reaction temperatures and multi-step sample preparations increases the costs and complexity of diagnostics, impeding their deployment in low-resource settings. Here, we develop a one-pot, room temperature recombinase polymerase amplification (RPA)-CRISPR reaction that removes these critical challenges. We show that RPA …

us (code pays fourni par la source)

Urine lipoarabinomannan concentrations among HIV-negative adults with pulmonary or extrapulmonary tuberculosis disease in Vietnam

Hoa Binh Nguyen, Mark Fajans, Hùng Nguyễn Văn, Bao Vu Ngoc et autres

Lipoarabinomannan (LAM) is a promising target biomarker for diagnosing subclinical and clinical tuberculosis (TB). Urine LAM (uLAM) testing using rapid diagnostic tests (RDTs) has been approved for people living with HIV (PLWH), however there is limited data regarding uLAM levels in HIV-negative …

vn, us, ch (code pays fourni par la source)

Real-world diagnostic accuracy of lipoarabinomannan in three non-sputum biospecimens for pulmonary tuberculosis disease

Xin Niu, Adrienne E. Shapiro, Zanele P. Magcaba, Zinhle Ngcobo et autres

Background Development of a non-sputum test using readily-obtainable biospecimens remains a global priority for tuberculosis (TB) control. We quantified lipoarabinomannan (LAM) concentrations, a pathogen biomarker for Mycobacterium tuberculosis , in urine, plasma and serum for real-world diagnostic accuracy of pulmonary TB among …

us, Afrique du Sud (code pays fourni par la source)

Correction: Performance of novel antibodies for lipoarabinomannan to develop diagnostic tests for Mycobacterium tuberculosis

Jason L. Cantera, Lorraine M. Lillis, Roger B. Peck, Emmanuel Moreau et autres

[This corrects the article DOI: 10.1371/journal.pone.0274415.].

ca, tw (code pays fourni par la source)

Urine lipoarabinomannan concentrations among HIV-uninfected adults with pulmonary or extrapulmonary tuberculosis disease in Vietnam

Mark Fajans, Hùng Nguyễn Văn, Bao Vu Ngoc, Hòa Nguyễn Thị et autres

Abstract Lipoarabinomannan (LAM) is a Mycobacterial cell wall glycolipid excreted in urine, and a target biomarker of rapid diagnostic tests (RDTs) for tuberculosis (TB) disease. Urine LAM (uLAM) testing by RDT has been approved for people living with HIV, but there is …

vn, us, ch (code pays fourni par la source)

Rapid Antigen and Antibody Microfluidic Immunofluorescence Assays Compared to Culture, PCR, and Laboratory Reference Tests: Performance in a Longitudinal Cohort

Ronit R. Dalmat, Linhui Hao, Roshni Prabhu, Elena Rechkina et autres

We evaluated the performance of rapid antigen (RAg) and antibody (RAb) microfluidic diagnostics with serial sampling of 71 participants at 6 visits over 2 months following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Rapid tests showed strong agreement with laboratory references …

us (code pays fourni par la source)

A Reagent and Virus Benchmarking Panel for a Uniform Analytical Performance Assessment of N Antigen-Based Diagnostic Tests for COVID-19

Allison Golden, Jason L. Cantera, Lorraine Lillis, Thanh T. Phan et autres

This study reports the results for severe acute respiratory syndrome coronavirus-2 (SARS-COV-2) nucleocapsid (N) and spike (S) antigen quantification assays and their performance against clinical reverse transcription (RT)-PCR results, thus describing an open-access quantification method for two important SARS-CoV-2 protein analytes. Characterized …

us (code pays fourni par la source)

Duration of viral infectiousness and correlation with symptoms and diagnostic testing in non-hospitalized adults during acute SARS-CoV-2 infection: A longitudinal cohort study

Paul K. Drain, Ronit R. Dalmat, Linhui Hao, Meagan J. Bemer et autres

BACKGROUND: Guidelines for SARS-CoV-2 have relied on limited data on duration of viral infectiousness and correlation with COVID-19 symptoms and diagnostic testing. METHODS: We enrolled ambulatory adults with acute SARS-CoV-2 infection and performed serial measurements of COVID-19 symptoms, nasal swab viral RNA, …

us (code pays fourni par la source)

Isolation and purification of lipoarabinomannan from urine of adults with active TB

Jason L. Cantera, Andrew Rashid, Lorraine M. Lillis, Roger B. Peck et autres

us, Afrique du Sud, jp, ch (code pays fourni par la source)