2023
conference-abstract
OpenAlex
Jeremy Michael Kinder, Christie Ciarlo, Chanel Athena Estrada, Neal Van Hoeven et autres
Abstract Background: We have developed a novel gene therapy platform based on fusogen biology that allows targeted delivery of CAR transgenes to “resting” T cells through systemic administration of a CD8-targeted paramyxovirus-based viral vector (VV). Therapies utilizing autologous CAR T cells have …
us
(code pays fourni par la source)
2021
conference-abstract
OpenAlex
Christie Ciarlo, Zach Frye, Andre DeGroot, Walter Flores et autres
Abstract Introduction: Chimeric antigen receptor T cell therapy (CAR T) is a successful treatment for B cell malignancies; however, the time, complexity and cost of manufacturing autologous CAR T products limits the availability of these therapies to patients. Furthermore, ex vivo manipulation …
us
(code pays fourni par la source)
Accès ouvert
2020
article
OpenAlex
Zibo Chen, Ryan Daniel Kibler, Andrew C. Hunt, Florian Büsch et autres
Designer gates Signaling in cells can occur through protein-protein interactions. Chen et al. describe the design of logic gates that can regulate protein association. The gates were built from small, designed proteins that all have a similar structure but where one module …
us
(code pays fourni par la source)
2020
article
OpenAlex
Matthew S. Wilken, Christie Ciarlo, Jocelynn R. Pearl, Jordan E. Bloom et autres
Non-invasive epigenome editing is a promising strategy for engineering gene expression programs, yet potency, specificity, and persistence remain challenging. Here we show that effective epigenome editing is gated at single-base precision via 'keyhole' sites in endogenous regulatory DNA. Synthetic repressors targeting promoter …
us
(code pays fourni par la source)
Accès ouvert
2020
preprint
OpenAlex
Matthew S. Wilken, Christie Ciarlo, Jocelynn R. Pearl, Elaine Schanzer et autres
Abstract Human genes are regulated quantitatively, yet the ability to specify the expression level of a native gene accurately and specifically using a defined reagent has remained elusive. Here we show that precise targeting of KRAB repressive domain within regulatory DNA unlocks …
us
(code pays fourni par la source)
Accès ouvert
2018
article
OpenAlex
Jonathan D. Mortison, Monica Schenone, Jacob A. Myers, Ziyang Zhang et autres
us, fr
(code pays fourni par la source)
Accès ouvert
2018
preprint
OpenAlex
Jonathan D. Mortison, Monica Schenone, Jacob A. Myers, Ziyang Zhang et autres
SUMMARY Apart from their antimicrobial properties, tetracyclines demonstrate clinically validated effects in the amelioration of pathological inflammation and human cancer. Delineation of the target(s) and mechanism(s) responsible for these effects, however, has remained elusive. Here, employing quantitative mass spectrometry-based proteomics, we identified …
us, fr
(code pays fourni par la source)
Accès ouvert
2018
preprint
OpenAlex
Jonathan D. Mortison, Monica Schenone, Jacob A. Myers, Ziyang Zhang et autres
us, fr
(code pays fourni par la source)
Accès ouvert
2017
article
OpenAlex
Christie Ciarlo, Charles K. Kaufman, Beste Kınıkoğlu, Michael Jonathan et autres
The neural crest is a dynamic progenitor cell population that arises at the border of neural and non-neural ectoderm. The inductive roles of FGF, Wnt, and BMP at the neural plate border are well established, but the signals required for subsequent neural …
us, nl
(code pays fourni par la source)
Accès ouvert
2017
peer-review
OpenAlex
Christie Ciarlo, Charles K. Kaufman, Beste Kınıkoğlu, Michael Jonathan et autres
us, nl
(code pays fourni par la source)
2017
dissertation
OpenAlex
Christie Ciarlo
The neural crest provides an excellent model for the study of cellular behavior during development. Arising at the border of neural and non-neural ectoderm, this dynamic cell population migrates throughout the embryo and differentiates into a variety of tissue types, including pigment …
2016
conference-abstract
OpenAlex
Charles K. Kaufman, Christian Mosimann, Andrew J. Thomas, Zi Peng Fan et autres
Abstract Within a group of cancer-prone cells that harbor a shared oncogenic mutation, only rare clones transition to a malignant state. These rare and transient events occurring during cancer initiation remain incompletely understood, and we thus sought to visualize and molecularly characterize …
us, ch, sg, de
(code pays fourni par la source)