Lucie Bonnet‐Madin

Unintegrated HIV-1 DNA recruits cGAS via its histone-binding domain to escape innate immunity

Cyprien Jahan, Lucie Bonnet‐Madin, Shinichi Machida, Bijan Sobhian et autres

To ensure optimal replication and spread, viruses have evolved countermeasures to evade type 1 IFN-mediated antiviral activity. During the early viral replication cycle steps until uncoating, the HIV-1 core protects viral pathogen associated molecular patterns (viral RNA and reverse transcription products) from …

fr, jp, us (code pays fourni par la source)

POLE3 is a repressor of unintegrated HIV-1 DNA required for efficient virus integration and escape from innate immune sensing

Suzie Thenin-Houssier, Shinichi Machida, Cyprien Jahan, Lucie Bonnet‐Madin et autres

Unintegrated retroviral DNA is transcriptionally silenced by host chromatin silencing factors. Here, we used the proteomics of isolated chromatin segments method to reveal viral and host factors associated with unintegrated HIV-1DNA involved in its silencing. By gene silencing using siRNAs, 46 factors …

fr, jp (code pays fourni par la source)

BK virus genotypes and humoral response in kidney transplant recipients with BKV associated nephropathy

Julien Gras, Marie Laure Néré, M.N. Peraldi, Lucie Bonnet‐Madin et autres

BACKGROUND: Among kidney transplant recipients (KTR) with BK virus associated nephropathy (BKVN), BKV genotypes' evolution and anti-BKV humoral response are not well established. We aim to analyze BKV replication and genetic evolution following transplantation, and characterize concomitant anti-BKV-VP1 humoral response. METHODS: We …

fr, us (code pays fourni par la source)

POLE3 is a transcriptional repressor of unintegrated linear HIV-1 DNA required for efficient virus integration and escape from innate immune sensing.

Monsef Benkirane, Suzie Thenin-Houssier, Cyprien Jahan, Shinichi Machida et autres

Abstract Unintegrated retroviral DNA are transcriptionally silent by mechanisms involving host chromatin silencing factors1–3. Here, we applied the proteomics of isolated chromatin segments approach (PICh)4 to reveal the proteomic of the linear unintegrated HIV-1 DNA (uniHIV-1 DNA) with the aim of identifying …

jp, fr (code pays fourni par la source)

Characterization and functional interrogation of the SARS-CoV-2 RNA interactome

Athéna Labeau, Luc Fery-Simonian, Alain Lefèvre‐Utile, Marie Pourcelot et autres

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the COVID-19 pandemic, which has led to a devastating global health crisis. The emergence of variants that escape neutralizing responses emphasizes the urgent need to deepen our understanding of SARS-CoV-2 …

fr (code pays fourni par la source)

RACK1 Associates with RNA-Binding Proteins Vigilin and SERBP1 to Facilitate Dengue Virus Replication

Alexis Brugier, Mohamed Lamine Hafirrassou, Marie Pourcelot, Morgane Baldaccini et autres

We recently identified the scaffolding RACK1 protein as an important host-dependency factor for dengue virus (DENV), a positive-stranded RNA virus responsible for the most prevalent mosquito-borne viral disease worldwide. Here, we have performed the first RACK1 interactome in human cells and identified …

fr, de (code pays fourni par la source)

RACK1 associates with RNA-binding proteins Vigilin and SERBP1 to control dengue virus replication

Alexis Brugier, Mohamed-Lamine Hafirrassou, Marie Pourcelot, Morgane Baldaccini et autres

Abstract Dengue virus (DENV), a re-emerging virus transmitted by Aedes mosquitoes, causes severe pathogenesis in humans. No effective treatment is available against this virus. We recently identified the scaffold protein RACK1 as a component of the DENV replication complex, a macromolecular complex …

fr, de (code pays fourni par la source)

Origine de la réactivation virale chez les patients transplantés rénaux avec une néphropathie à BK virus

Julien Gras, Marie Laure Néré, Lucie Bonnet‐Madin, Maud Salmona et autres

Introduction Après transplantation rénale, une néphropathie à BK virus (BKVN) survient dans 1 à 10 % des cas et représente une cause majeure de dysfonction du greffon. Plusieurs études suggèrent que le greffon constituerait la principale source de transmission du virus. Méthodes …

fr (code pays fourni par la source)

Origine de la réactivation virale chez les patients transplantés rénaux avec une néphropathie à BK virus

Julien Gras, Marie Laure Néré, Lucie Bonnet‐Madin, Maud Salmona et autres

fr (code pays fourni par la source)

FHL1 is a key player of chikungunya virus tropism and pathogenesis

Laurent Meertens, Mohamed Lamine Hafirassou, Thérèse Couderc, Lucie Bonnet‐Madin et autres

Chikungunya is an infectious disease caused by the chikungunya virus (CHIKV), an alphavirus transmitted to humans by Aedes mosquitoes, and for which there is no licensed vaccine nor antiviral treatments. By using a loss-of-function genetic screen, we have recently identified the FHL1 …

fr, de (code pays fourni par la source)

Characterization and functional interrogation of SARS-CoV-2 RNA interactome

Athéna Labeau, Alain Lefèvre‐Utile, Lucie Bonnet‐Madin, Luc Fery-Simonian et autres

SUMMARY Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19 pandemic, which has caused a devastating global health crisis. The emergence of highly transmissible novel viral strains that escape neutralizing responses emphasizes the urgent need to deepen our …

fr (code pays fourni par la source)

SARS-CoV-2 induces human plasmacytoid predendritic cell diversification via UNC93B and IRAK4

Fanny Onodi, Lucie Bonnet‐Madin, Laurent Meertens, Léa Karpf et autres

Several studies have analyzed antiviral immune pathways in late-stage severe COVID-19. However, the initial steps of SARS-CoV-2 antiviral immunity are poorly understood. Here we have isolated primary SARS-CoV-2 viral strains and studied their interaction with human plasmacytoid predendritic cells (pDCs), a key …

fr, us (code pays fourni par la source)