Erica A. Green

Development of an HEK293 Suspension Cell Culture Medium, Transient Transfection Optimization Workflow, and Analytics for Batch rAAV Manufacturing

Erica A. Green, Qiang Fu, Nelson Ndhairo, Thomas M Leibiger et autres

Recombinant adeno associated virus (rAAV) vectors have become popular delivery vehicles for in vivo gene therapies, but demand for rAAVs continues to outpace supply. Platform processes for rAAV production are being developed by many manufacturers, and transient chemical transfection of human embryonic …

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Biolayer interferometry for adeno-associated virus capsid titer measurement and applications to upstream and downstream process development

Thomas M Leibiger, Luke A. Remmler, Erica A. Green, Kelvin H. Lee

Faster and more accurate analytical methods are needed to support the advancement of recombinant adeno-associated virus (rAAV) production systems. Recently, biolayer interferometry (BLI) has been developed for high-throughput AAV capsid titer measurement by functionalizing the AAVX ligand onto biosensor probes (AAVX-BLI). In …

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Design space determination to optimize DNA complexation and full capsid formation in transient rAAV manufacturing

Qiang Fu, Yong Suk Lee, Erica A. Green, Yongdan Wang et autres

Recombinant adeno-associated virus (rAAV) vectors are a promising platform for in vivo gene therapies. However, cost-effective, well-characterized processes necessary to manufacture rAAV therapeutics are challenging to develop without an understanding of how process parameters (PPs) affect rAAV product quality attributes (PQAs). In …

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Analytical methods to characterize recombinant adeno-associated virus vectors and the benefit of standardization and reference materials

Erica A. Green, Kelvin H. Lee

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