Li Fan

Surface Engineering of Glutamic Acid Residues to Enhance the Thermostability of GH10 Xylanases

Jingyi Tian, Xueting Qu, Wenjing Huang, Ailing Sun et autres

Thermostability is critical for the industrial applications of xylanase, including paper production, animal feed, and lignocellulosic biomass conversion. Here, we report that the rational introduction of surface-exposed glutamic acid (Glu) residues significantly enhances the thermostability of GH10 xylanases. Engineered variants of XT6, …

cn, sg, Malawi (code pays fourni par la source)

A Cold- and Salt-Tolerant GH43-1 β-Xylosidase from Cellulomonas bogoriensis 69B4T: Biochemical Characterization and Subfamily Functional Diversity

Xueting Qu, S K Wang, Shanshan Gao, Yuchi Zhou et autres

cn, kn, bd (code pays fourni par la source)

Preparation of RGD-modified liposomes encapsulated with shikonin and its targeted anti-melanoma effects

Hao Zhang, Ying Zhao, Tingting Chen, Xinliang Mao et autres

Melanoma is the most aggressive skin tumor, and conventional treatment is ineffective. Studies have shown that shikonin, derived from the traditional Chinese medicine Lithospermum erythrorhizon , has various anticancer activities. In this work, RGD- modified liposomes encapsulated with shikonin (RGD-Lip-SHK) were prepared …

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RAD51 expression and prognostic impact in patients with stomach adenocarcinoma

Baiyu Jian, Hao Zhang, Li Fan, Yang Li et autres

Background: Stomach adenocarcinoma (STAD) is the most common gastrointestinal cancer. A clear diagnosis and molecular targeted therapy have important implications for prolonging survival of patients. RAD51 is the central catalyst of homologous recombination that plays important role in maintaining genomic integrity. However, …

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Structural Analysis and Antioxidant Activity of Alkaline-Extracted Glucans from Hericium erinaceus

Zhonghui Qiao, Xiushi Jia, Yuanning Wang, Yuan Wang et autres

An alkali-soluble β-glucan (AHEP-A-b, 20 kDa) purified from Hericium erinaceus fruiting bodies, was structurally characterized and examined for antioxidant activity. Methylation analysis and NMR spectroscopy show that the backbone of AHEP-A-b is composed of (1→6)-linked-D-β-glucopyran residues, branched at O-3 of glucopyranose (Glcp) …

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Preparation, in vitro anti-tumour activity and in vivo pharmacokinetics of RGD-decorated liposomes loaded with shikonin

Jiping Li, Hao Zhang, Xinliang Mao, Huilin Deng et autres

Shikonin (SHK) has been evidenced to possess effects against various cancer cells. However, poor aqueous solubility and high toxicity restrict its application. In the study, RGD-decorated liposomes loaded with SHK (RGD-Lipo-SHK) were prepared via thin-film hydration method. Characterization and cellular uptake of …

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Effects of Medium Additives on the Mycelial Growth and Polysaccharide Biosynthesis in Submerged Culture of Bjerkandera fumosa

Li Fan, Huizhen Fan, Qianwen Sun, Di Yao et autres

Medium additives have been shown to affect the synthesis of active products in fungi. This study investigated the effects of corn stalk, poplar sawdust, Tween-80, and oleic acid on mycelial biomass and physicochemical properties, as well as the bioactivity of polysaccharides, including …

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Characterization of the complete mitochondrial genome sequence of Tanichthys albiventris and phylogenetic analysis

Tiezhu Yang, Chenxi Tan, Wenhao Sun, Dongpu Li et autres

In this study, we sequenced, annotated, and characterized the mitogenome of Tanichthys albiventris for the first time. The complete mitogenome is 16,544 bp in length, consisting of 13 protein-coding genes (PCGs), 22 transfer RNA genes, and two ribosomal RNA genes. We found …

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Novel Isoindolinone-Based Analogs of the Natural Cyclic Peptide Fenestin A: Synthesis and Antitumor Activity

Huanli Zhang, Jingwan Wu, Jingchun Wang, Shimei Xiao et autres

Small- and medium-sized cyclopeptides have been found to have extensive bioactivities and have drawn much attention from medicinal chemists. In the work described in this paper, various cyclic peptide analogs of Fenestin A were synthesized by intramolecular photoinduced electron-transfer cyclization reactions to …

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Jolkinolide B induces cell cycle arrest and apoptosis in MKN45 gastric cancer cells and inhibits xenograft tumor growth in vivo

Hao Zhang, Jiayi Qian, Ming Hao Jin, Li Fan et autres

Gastric cancer is one of the most common digestive carcinomas throughout the world and represents high mortality. There is an urgent quest for seeking a novel and efficient antigastric cancer drug. Euphorbia fischeriana Steud had long been used as a traditional Chinese …

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Genipin protects against mitochondrial damage of the retinal pigment epithelium under hyperglycemia through the AKT pathway mediated by the miR-4429/JAK2 signaling axis

Wenshuang Xu, Qingyou Chen, Xiaofeng Zhang, Yue Zhao et autres

Background: To investigate the protective effect and mechanism of genipin (GE) on mitochondrial damage in retinal pigment epithelial (RPE) cells induced by high glucose. Methods: Differential genes of GE in the treatment of diabetic retinopathy (DR) were screened by the Gene Expression …

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The Pyocin Regulator PrtR Regulates Virulence Expression of Pseudomonas aeruginosa by Modulation of Gac/Rsm System and c-di-GMP Signaling Pathway

Hongying Jiao, Li Fan, Tietao Wang, Joey Kuok Hoong Yam et autres

In Pseudomonas aeruginosa , the second messenger cyclic-di-GMP and Gac/Rsm signaling pathways are associated with the transition from acute to chronic infection. Therefore, identification of the molecular mechanisms that govern lifestyle choice in bacteria is very important.

cn, sg (code pays fourni par la source)