Kateřina Plachká

Comprehensive investigation of matrix effect evaluation approaches for reliable quantification in bioanalysis using UHPLC-MS/MS

Hana Vlčková, Kateřina Plachká, Frantisek Švec, Lucie Nováková

BACKGROUND: Matrix effect (ME) evaluation is an indispensable part of the method development and validation when using ultra-high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS). The rotational and translational matrix effects are often observed. The rotational matrix effect depends on the …

cz (code pays fourni par la source)

Data set for Comprehensive Investigation of Matrix Effect Evaluation Approaches for Reliable Quantification in Bioanalysis using UHPLC-MS/MS

Hana Vlčková, Kateřina Plachká, František Švec, Lucie Nováková

The main objective of this study was to systematically evaluate two quantitative approaches for matrix effect evaluation, i.e., the post-extraction addition and slope-based approach, using serum as a complex matrix and a non-selective protein precipitation procedure. Based on the observed limitations of …

cz (code pays fourni par la source)

Data set for Comprehensive Investigation of Matrix Effect Evaluation Approaches for Reliable Quantification in Bioanalysis using UHPLC-MS/MS

Hana Vlčková, Kateřina Plachká, František Švec, Lucie Nováková

The main objective of this study was to systematically evaluate two quantitative approaches for matrix effect evaluation, i.e., the post-extraction addition and slope-based approach, using serum as a complex matrix and a non-selective protein precipitation procedure. Based on the observed limitations of …

cz (code pays fourni par la source)

Supercritical fluid chromatography hyphenated with dielectric barrier discharge ionization mass spectrometry: A universal approach to analysis of complex plant extracts

Kateřina Plachká, Karel Vraj, Veronika Pilařová, Lucie Nováková

cz (code pays fourni par la source)

Mobile phase modifiers and additives in supercritical fluid chromatography

Veronika Pilařová, Kateřina Plachká, Lucie Nováková

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List of contributors

Fardine Ameli, M. Ashraf-Khorassani, Takeshi Bamba, Rodell C. Barrientos et autres

Ultra high-performance supercritical fluid chromatography-mass spectrometry emerging as a complementary approach for targeted steroid analysis

Taťána Gazárková, Kateřina Plachká, Hana Vlčková, Lucie Nováková

cz (code pays fourni par la source)

AI-powered insights into the UniSpray ionization in supercritical fluid chromatography-mass spectrometry

Veronika Pilařová, Kateřina Plachká, Taťána Gazárková, František Švec et autres

cz, fr (code pays fourni par la source)

Artificial Neural Networks: An Innovative Approach Used for Elucidation of Ionization Processes in Supercritical Fluid Chromatography-Mass Spectrometry

Kateřina Plachká, Veronika Pilařová, Taťána Gazárková, Jean‐Christophe Garrigues et autres

High Resolution Image Download MS PowerPoint Slide Understanding and predicting mass spectrometry responses in supercritical fluid chromatography-mass spectrometry (SFC-MS) is critical for optimizing detection across diverse analytes and solvent compositions. We present a novel approach using artificial neural networks (ANN) to explore …

cz, fr (code pays fourni par la source)

Microflow LC-MS Bottom-Up Proteomics Using 1.5 mm Internal Diameter Columns

Siddharth Jadeja, Denis K. Naplekov, Mykyta R. Starovoit, Kateřina Plachká et autres

Microbore columns with a 1.0 mm inner diameter (i.d.) have gained popularity in microflow liquid chromatography-mass spectrometry (LC-MS) workflows for exploratory proteomics applications due to their high throughput, robustness, and reproducibility. However, obtaining highly efficient separation using these columns remains unachievable, primarily …

cz, us (code pays fourni par la source)

AI-Enhanced Understanding of Retention Interactions in Supercritical Fluid Chromatography: Neural Network Insights into Retention on Selected Non-Polar Stationary Phases

Kateřina Plachká, Veronika Pilařová, Taťána Gazárková, František Švec et autres

High Resolution Image Download MS PowerPoint Slide The retention behavior in supercritical fluid chromatography (SFC) remains a complex and poorly understood phenomenon despite the development of various models to explain retention mechanisms. This study aims to deepen the understanding of retention by …

cz, fr (code pays fourni par la source)

Comprehensive targeted profiling of multiple steroid classes in rodent plasma using liquid chromatography-mass spectrometry

Taťána Gazárková, Hana Vlčková, Kateřina Plachká, Karla Vagnerová et autres

cz (code pays fourni par la source)