David Lonsway

Identification and Characterization of Vancomycin-Resistant Staphylococcus aureus CC45/USA600, North Carolina, USA, 2021

Jennifer MacFarquhar, Anumita Bajpai, Teresa A. Fisher, Alyssa G. Kent et autres

Vancomycin-resistant Staphylococcus aureus (VRSA) is a rare but serious public health concern. We describe a VRSA case in North Carolina, USA. The isolate from the case belonged to the USA600 lineage and clonal complex 45. No transmission was identified. Confirmed VRSA cases …

2747. Aztreonam-Avibactam Susceptibility Testing Program for Metallo-β-Lactamase-Producing Enterobacterales collected through the Antimicrobial Resistance Laboratory Network, March 2019 to April 2023

Amelia Bhatnagar, Porscha Bumpus-White, Sarah Sabour, Janine Bodnar et autres

Abstract Background Metallo-β-lactamase-producing Enterobacterales (MBL-E) are a major public health concern as they cause infections with few effective treatment options. Aztreonam-avibactam (AZA) is a β-lactam/β-lactamase inhibitor combination agent in the drug development pipeline that is active against MBL-E. Though clinical studies are …

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Antimicrobial Resistance Laboratory Network’s multisite evaluation of the ThermoFisher Sensititre GN7F broth microdilution panel for antimicrobial susceptibility testing

Amelia Bhatnagar, María-José Machado, Logan Patterson, Karen Anderson et autres

ABSTRACT In 2017, the Centers for Disease Control and Prevention (CDC) established the Antimicrobial Resistance Laboratory Network to improve domestic detection of multidrug-resistant organisms. CDC and four laboratories evaluated a commercial broth microdilution panel. Antimicrobial susceptibility testing using the Sensititre GN7F (ThermoFisher …

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Preparation of Routine Media and Reagents Used in Antimicrobial Susceptibility Testing

David Lonsway

Abstract McFarland turbidity standards are used to standardize the approximate number of the book bacteria in a liquid suspension by visually comparing the turbidity of a test suspension with the turbidity of a McFarland standard. Aliquots of antimicrobial stock solutions are incorporated …

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Preparation of Broth Microdilution MIC Trays

David Lonsway

Abstract Broth microdilution MIC trays may be prepared in‐house for performance of quantitative antimicrobial susceptibility studies. The medium preparation would be similar to what is used for conventional MICs, but there are a few differences in the MIC plate preparation. These include …

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Development of a Broth Microdilution Method To Characterize Chlorhexidine MICs among Bacteria Collected from 2005 to 2019 at Three U.S. Sites

Joseph D. Lutgring, Julian E. Grass, David Lonsway, Byong Kwon Yoo et autres

Chlorhexidine bathing to prevent transmission of multidrug-resistant organisms and reduce health care-associated infections has been adopted by many hospitals. There is concern about the possible unintended consequences of using this agent widely. One possible unintended consequence is decreased susceptibility to chlorhexidine, but …

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Welder’s Anthrax: A Tale of 2 Cases

Katherine Hendricks, Roosecelis B. Martines, Hannah Bielamowicz, Anne E. Boyer et autres

Bacillus anthracis has traditionally been considered the etiologic agent of anthrax. However, anthrax-like illness has been documented in welders and other metal workers infected with Bacillus cereus group spp. harboring pXO1 virulence genes that produce anthrax toxins. We present 2 recent cases …

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Evaluation of MALDI Biotyper Mycobacteria Library for Identification of Nontuberculous Mycobacteria

Nadege C. Toney, Wenming Zhu, Bette Jensen, Jarrett Gartin et autres

The Bruker Biotyper matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) platform was assessed on its ability to accurately identify 314 nontuberculous mycobacteria (NTM) representing 73 species. All NTM isolates, representing 183 rapidly growing and 131 slowly growing organisms, were previously identified …

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Molecular Characterization of Carbapenem-Resistant Enterobacterales Collected in the United States

Maria Karlsson, Joseph D. Lutgring, Uzma Ansari, Adrian Lawsin et autres

Carbapenem-resistant Enterobacterales (CRE) are a growing public health concern due to resistance to multiple antibiotics and potential to cause health care-associated infections with high mortality. Carbapenemase-producing CRE are of particular concern given that carbapenemase-encoding genes often are located on mobile genetic elements …

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Aztreonam-Avibactam Susceptibility Testing Program for Metallo-Beta-Lactamase-Producing Enterobacterales in the Antibiotic Resistance Laboratory Network, March 2019 to December 2020

Amelia Bhatnagar, Sandra Boyd, Sarah Sabour, Janine Bodnar et autres

Aztreonam-avibactam is a drug combination pending phase 3 clinical trials and is suggested for treatment of severe infections caused by metallo-beta-lactamase (MBL)-producing Enterobacterales by combining ceftazidime-avibactam and aztreonam. Beginning in 2019, four Antibiotic Resistance Laboratory Network regional laboratories offered aztreonam-avibactam susceptibility testing …

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Acquisition of Ciprofloxacin Resistance Among an Expanding Clade of β-Lactamase–Positive, Serogroup Y Neisseria meningitidis in the United States

Caelin Cubeñas-Potts, Adam C. Retchless, Lucy A. McNamara, Daya Marasini et autres

BACKGROUND: Penicillin and ciprofloxacin are important for invasive meningococcal disease (IMD) management and prevention. IMD cases caused by penicillin- and ciprofloxacin-resistant Neisseria meningitidis containing a ROB-1 β-lactamase gene (blaROB-1) and a mutated DNA gyrase gene (gyrA) have been recently reported in the …

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A Comparison of Less Invasive Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Diagnostic Specimens in Nursing Home Residents—Arkansas, June–August 2020

Paige Gable, J. Huang, Sarah Gilbert, Susan Bollinger et autres

BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing remains essential for early identification and clinical management of cases. We compared the diagnostic performance of 3 specimen types for characterizing SARS-CoV-2 in infected nursing home residents. METHODS: A convenience sample of 17 …

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