Preparation of Routine Media and Reagents Used in Antimicrobial Susceptibility Testing

Le résumé fourni par la source

Abstract McFarland turbidity standards are used to standardize the approximate number of the book bacteria in a liquid suspension by visually comparing the turbidity of a test suspension with the turbidity of a McFarland standard. Aliquots of antimicrobial stock solutions are incorporated into broth or agar medium for antimicrobial susceptibility tests. Stock solutions are prepared from laboratory reference standard‐grade antimicrobial powders. These are assayed for the amount of active agent present, and this is expressed as potency or activity, which is usually defined in terms of micrograms of active drug per milligram. Specific agar and broth media used for routine disk diffusion, agar dilution, and broth dilution susceptibility tests are prepared from commercially available dehydrated components. Although these media have shown fairly good reproducibility through many years of testing, they are undefined media, and there may be subtle performance differences among batches and lots.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.