Accès ouvert
2026
article
OpenAlex
Ramón Korn, Florian Weiß, Markus C. Berg, Benedikt Haslinger et autres
Plasmid DNA is a critical raw material for genetic medicines, including viral vectors and mRNA vaccines, yet its primary recovery remains a significant downstream bottleneck. Overcoming these current limitations is essential to meet the rapidly growing demand for genetic medicines while increasing …
at, si
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Accès ouvert
2026
preprint
OpenAlex
Benedikt Haslinger, Barbara Reischl, Franziska Steger, Maximilian Krippl et autres
Abstract Methanogenic archaea, such as Methanothermobacter marburgensis, represent a powerful biological platform for carbon capture and valorization, directly converting carbon dioxide (CO2) and molecular hydrogen (H2) into proteinogenic amino acids (AAs). In this study, we present a controlled and scalable strategy for …
at
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Accès ouvert
2025
article
OpenAlex
Evan Claes, Tommy Heck, Roger Dalmau‐Diaz, Mark Duerkop et autres
The intensification of cell & gene therapy manufacturing processes is crucial for their industrial translation and for addressing large patient populations at a sustainable price, as corroborated by recent communications by the FDA. Achieving this requires innovative process development strategies to reduce …
be, at
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Accès ouvert
2023
article
OpenAlex
Verena Nold, Lisa Junghans, Benjamin Bayer, Lorenzo Bisgen et autres
Introduction: For the implementation of robust bioprocesses, understanding of temporal cell behavior with respect to relevant inputs is crucial. Intensified Design of Experiments (iDoE) is an efficient tool to assess the joint influence of input parameters by including intra-experimental changes. Methods: We …
de
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Accès ouvert
2022
article
OpenAlex
Benjamin Bayer, Mark Duerkop, Ralf Pörtner, Johannes Möller
Despite the advantages of mathematical bioprocess modeling, successful model implementation already starts with experimental planning and accordingly can fail at this early stage. For this study, two different modeling approaches (mechanistic and hybrid) based on a four-dimensional antibody-producing CHO fed-batch process are …
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Accès ouvert
2022
article
OpenAlex
Thomas Perkmann, Thomas Köller, Nicole Perkmann‐Nagele, Mária Ozsvár-Kozma et autres
BACKGROUND: Serological tests are widely used in various medical disciplines for diagnostic and monitoring purposes. Unfortunately, the sensitivity and specificity of test systems are often poor, leaving room for false-positive and false-negative results. However, conventional methods were used to increase specificity and …
at, gb
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Accès ouvert
2022
article
OpenAlex
Michele Atzl, Axel Muendlein, Thomas L. Winder, Peter Fraunberger et autres
Objectives Austria, and particularly its westernmost federal state Vorarlberg, developed an extremely high incidence rate during the COVID-19 pandemic. Healthcare workers (HCWs) worldwide are known to have an increased risk of contracting the disease within the working environment and, therefore, the seroprevalence …
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Accès ouvert
2021
article
OpenAlex
Benjamin Bayer, Mark Duerkop, Gerald Striedner, Bernhard Sissolak
Reliable process development is accompanied by intense experimental effort. The utilization of an intensified design of experiments (iDoE) (intra-experimental critical process parameter (CPP) shifts combined) with hybrid modeling potentially reduces process development burden. The iDoE can provide more process response information in …
at
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Accès ouvert
2021
article
OpenAlex
Benjamin Bayer, Andreas Maccani, Johanna A. Jähn, Mark Duerkop et autres
OBJECTIVES: The applicability of proton-transfer-reaction mass spectrometry (PTR-MS) as a versatile online monitoring tool to increase consistency and robustness for recombinant adeno-associated virus (rAAV) producing HEK 293 bioprocesses was evaluated. We present a structured workflow to extract process relevant information from PTR-MS …
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Accès ouvert
2021
article
OpenAlex
Maximilian Krippl, Tobias Kargl, Mark Duerkop, Astrid Dürauer
Single-pass tangential flow filtration (SPTFF) is a promising and increasingly important tool for continuous biomanufacturing and intensified bioprocessing. SPTFF operates at high product concentrations often limiting the number of process development experiments and therefore process understanding. We developed hybrid model structures to …
at
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Accès ouvert
2021
article
OpenAlex
Benjamin Bayer, Roger Dalmau Diaz, Michael Melcher, Gerald Striedner et autres
The fast exploration of a design space and identification of the best process conditions facilitating the highest space-time yield are of great interest for manufacturers. To obtain this information, depending on the design space, a large number of practical experiments must be …
at
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Accès ouvert
2021
article
OpenAlex
Thomas Perkmann, Thomas Köller, Nicole Perkmann‐Nagele, Miriam Klausberger et autres
SARS-CoV
at
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