Stacey LaFrentz

An Optimized Method for Efficient Conjugal Transfer in Flavobacterium covae

Stacey LaFrentz, Hannah Smith, Benjamin R. LaFrentz, Jake Tatum et autres

ABSTRACT The fish pathogen Flavobacterium covae causes significant mortality and there is a need to better understand virulence factors that contribute to its pathogenesis. Few highly virulent F. covae strains may be genetically manipulated with existing conjugal transfer methods, therefore the aim …

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Probiotics enhance resistance to Streptococcus iniae in Nile tilapia (Oreochromis niloticus) reared in biofloc systems

Uthpala Padeniya, D. Allen Davis, Mark R. Liles, Stacey LaFrentz et autres

Abstract Biofloc technology is a rearing technique that maintains desired water quality by manipulating carbon and nitrogen and their inherent mixture of organic matter and microbes. Beneficial microorganisms in biofloc systems produce bioactive metabolites that may deter the growth of pathogenic microbes. …

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Corynebacterium conjunctivae: A New Corynebacterium Species Isolated from the Ocular Surface of Healthy Horses

José Francisco Fernández‐Garayzábal, Stacey LaFrentz, A. Casamayor, Eva Abarca et autres

Twenty-two unidentified Gram-positive, rod-shaped organisms were recovered from the conjunctival surface of apparently healthy horses and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Corynebacterium, although they did not …

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Draft Genome Sequences of Cetobacterium somerae 2G Large and Two Novel Cetobacterium Isolates from Intestines of Channel Catfish ( Ictalurus punctatus )

Benjamin R. LaFrentz, Stacey LaFrentz, Benjamin H. Beck, Covadonga R. Arias

We report the draft genome sequences of Cetobacterium somerae 2G Large and two Cetobacterium isolates, 2A and 8H, which may represent novel species. The isolates were recovered from the intestines of catfish, and the genomes will assist in research to understand their …

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Characterization of the normal equine conjunctival bacterial community using culture‐independent methods

Stacey LaFrentz, Eva Abarca, Haitham H. Mohammed, Rosemary Cuming et autres

BACKGROUND: The equine conjunctival microbiota has often been reported to be dominated by Gram-positive species such as Staphylococcus sp., Bacillus sp., and Corynebacterium sp. However, traditional culture-based methods can only recover a fraction of the bacterial species present in the sample. OBJECTIVES: …

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Covadonga (Cova) Rodrigues Arias, 1970–2019

Stacey LaFrentz, Benjamin R. LaFrentz

Journal of Aquatic Animal HealthVolume 31, Issue 3 p. 299-299 In Memoriam Covadonga (Cova) Rodrigues Arias, 1970–2019 Stacey A. LaFrentz, Stacey A. LaFrentz Aquatic Microbiology Laboratory, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, Alabama, 36849 USASearch for more papers …

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Description and characterization of the digestive gland microbiome in the freshwater mussel Villosa nebulosa (Bivalvia: Unionidae)

Alison K Aceves, Paul D. Johnson, Stephen A. Bullard, Stacey LaFrentz et autres

Here we characterize the digestive gland microbiome from wild and cultured (hatchery-reared) Alabama rainbows (Villosa nebulosa) using 16 S rRNA gene pyrosequencing in order to understand the effects of propagation on microbial community structure in freshwater mussels. Digestive glands from nine Alabama …

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Morphological and molecular confirmation of Myxobolus cerebralis myxospores infecting wild‑caught and cultured trout in North Carolina (SE USA)

Carlos F. Ruiz, Jacob M. Rash, Cova R. Arias, Doug A. Besler et autres

We used microscopy and molecular biology to provide the first documentation of infections of Myxobolus cerebralis (Myxozoa: Myxobolidae), the etiological agent of whirling disease, in trout (Salmonidae) from North Carolina (USA) river basins. A total of 1085 rainbow trout Oncorhynchus mykiss, 696 …

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New attenuated vaccine against columnaris disease in fish: Choosing the right parental strain is critical for vaccine efficacy

Haitham H. Mohammed, Óscar Olivares-Fuster, Stacey LaFrentz, Covadonga R. Arias

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Adaptive response to starvation in the fish pathogen Flavobacterium columnare: cell viability and ultrastructural changes

Covadonga R. Arias, Stacey LaFrentz, Wenlong Cai, Óscar Olivares-Fuster

BACKGROUND: The ecology of columnaris disease, caused by Flavobacterium columnare, is poorly understood despite the economic losses that this disease inflicts on aquaculture farms worldwide. Currently, the natural reservoir for this pathogen is unknown but limited data have shown its ability to …

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Additional file 1

Covadonga R. Arias, Stacey LaFrentz, Wenlong Cai, Óscar Olivares-Fuster

Impact of Compounding Error on Strategies for Subtyping Pathogenic Bacteria

Douglas R. Call, Lisa H. Orfe, Margaret A. Davis, Stacey LaFrentz et autres

Comparative-omics will identify a multitude of markers that can be used for intraspecific discrimination between strains of bacteria. It seems intuitive that with this plethora of markers we can construct higher resolution subtyping assays using discrete markers to define strain "barcodes." Unfortunately, …

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